Aspergillus awamori strain and application thereof in field of preventing and controlling of agricultural pests

A technology of Aspergillus awamori and Aspergillus awamori, which is applied to Aspergillus awamori strains and its application in the field of agricultural pest control and biological pesticides. It can solve the problems of destroying ecological balance, endangering the ecological environment of grasslands, polluting grasslands, etc., and achieves a strong lethal effect and is widely used foreground effect

Active Publication Date: 2017-08-18
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemical pesticides have disadvantages such as high cost, large dosage, and poor safety, and large-scale use can easily cause human and animal poisoning, pollute grasslands, and kill natural enemies of locusts, thereby endangering the ecological environment of grasslands and destroying ecological balance.

Method used

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  • Aspergillus awamori strain and application thereof in field of preventing and controlling of agricultural pests
  • Aspergillus awamori strain and application thereof in field of preventing and controlling of agricultural pests
  • Aspergillus awamori strain and application thereof in field of preventing and controlling of agricultural pests

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Isolation, screening and identification of Aspergillus awamori

[0040] 1. Medium

[0041] Potato dextrose agar medium (PDA): 200 g of potatoes, 20 g of anhydrous glucose, 20 g of agar powder, and 1000 ml of distilled water.

[0042] 2. Screening and purification of bacterial strains

[0043] (1) Source of bacteria

[0044] The source of bacteria was collected from 26 soil samples collected at a distance of 3 to 6 cm from the surface in Ganzi Prefecture, Sichuan Province and Dabei Mountain in Urumqi.

[0045] (2) Isolation and purification of bacterial strains

[0046] Accurately weigh 10 g of the soil sample, add it to a conical flask filled with 90 ml of sterile water and glass beads, and shake for about 20 minutes to fully mix the soil sample with the water and disperse the cells. Prepare a series of soil dilutions with concentration gradients, and then absorb 1ml each and spread the bacterial solution evenly on the plate with a sterile glass smear stic...

Embodiment 2

[0052] Embodiment 2 Preparation of Aspergillus awamori fungal fermentation broth and Aspergillus awamori metabolites

[0053] The isolated fungus was inoculated into 500 ml of sterile liquid PDA medium containing 250 ml, 165 r / min, 26 ° C shaking culture for 7 to 14 days to obtain 80 L of fungal fermentation broth.

[0054] Use filter paper to filter and centrifuge at 10,000 rpm at 4°C to obtain the supernatant of the fermentation broth. Add 95% ethanol according to the ratio of fermented liquid and solvent 1:3 (W / V), cold soak at room temperature for 24 hours, stir and extract in a water bath at 50°C for 6 hours, and then filter with suction to obtain a dark brown ethanol extract, and extract in this way for 3 times, The combined ethanol extracts were concentrated by a rotary evaporator to prepare a dark brown pasty ethanol crude extract, which is the metabolite of Aspergillus awamori.

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Abstract

The invention relates to an aspergillus awamori strain and application thereof in the field of preventing and controlling of agricultural pests, and belongs to the technical field of biological pesticide, which aims at providing the aspergillus awamori strain which is capable of being applied into biological locust elimination. The aspergillus awamori strain is preserved into China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.13363. The aspergillus awamori strain and a metabolite thereof have the advantages that the locust can be killed, the stronger killing function on oriental migratory locust, and the broad application prospect is realized in the field of biological pesticide.

Description

technical field [0001] The invention relates to Aspergillus awamori strain and its application in the field of agricultural pest control, and belongs to the technical field of biological pesticides. Background technique [0002] Locusts, commonly known as "grasshoppers", belong to the order Orthoptera, including Tetrigoidea, Eumastacoidea, and Locustoidea. There are more than 10,000 species in the world, and more than 1,000 species in my country. Distributed in tropical and temperate grasslands and deserts all over the world. Locusts mainly include migratory locusts and soil locusts. In my country, there are three species of migratory locusts: Locusta migratoria manilensis (Meyen), Locusta migratoria migratoria (Linnaeus) and Locusta migratoria tibitensis Chen, among which the distribution of locusts in my country is the widest. , the most serious damage, is the most important species of migratory locusts causing locust disasters in my country. [0003] Grassland locusts ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/665
CPCA01N63/30C12N1/14C12N1/145C12R2001/665
Inventor 侯太平查艳梅陶科金洪岳莹刘英
Owner SICHUAN UNIV
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