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Preparation method of alfalfa rhizobia immobilized embedded granule formation and application thereof

A technology of alfalfa and rhizobia, which is applied in the field of agricultural microorganism application, can solve the problems of inconvenient addition and use, difficulty in maintaining the vitality of alfalfa rhizobia protective agents, etc., and achieve the effect of easy use

Inactive Publication Date: 2017-05-31
DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It has broken through the main technical bottlenecks and difficulties of the high-tolerance and high-vigority Rhizobia alfalfa seed coating technology, and solved the technical problems of difficulty in maintaining the vitality of the Rhizobia alfalfa protective agent and inconvenient addition and use

Method used

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  • Preparation method of alfalfa rhizobia immobilized embedded granule formation and application thereof
  • Preparation method of alfalfa rhizobia immobilized embedded granule formation and application thereof
  • Preparation method of alfalfa rhizobia immobilized embedded granule formation and application thereof

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specific Embodiment approach 1

[0022] Specific embodiment one: the preparation method of the rhizobium alfalfa immobilized embedding granule dosage form in the present embodiment is carried out according to the following steps:

[0023] Step 1, cultivating Rhizobium alfalfa: Utilize YMA liquid medium to cultivate Rhizobium alfalfa until the number of live bacteria reaches 1×10 9 More than one / ml, made into alfalfa rhizobium bacteria liquid;

[0024] Step 2, centrifugal separation of Rhizobium alfalfa bacteria liquid: divide the Rhizobium alfalfa bacteria liquid prepared in step 1 into 10ml of bacteria liquid, and centrifuge. The rotating speed of the centrifuge is 10000 rpm, and the centrifugal time is 5min. After separation, remove the separation supernatant, add fresh YMA liquid medium to the separated rhizobium meliloti liquid medium to 10ml, mix well, and centrifuge again, add fresh YMA medium to the separated rhizobia meliloti bottom liquid YMA liquid medium to 10ml, mix well, centrifuge again, add fr...

specific Embodiment approach 2

[0030] Specific embodiment two: in the present embodiment, the preparation method of the rhizobia meliloti immobilized embedding granule dosage form is carried out according to the following steps:

[0031] Step 1, cultivating Rhizobium alfalfa: Utilize YMA solid medium to cultivate Rhizobium alfalfa until the number of viable bacteria reaches 1×109 More than one / ml, made into alfalfa rhizobium bacteria liquid;

[0032] Step 2, centrifugal separation of Rhizobium alfalfa bacteria liquid: divide the Rhizobium alfalfa bacteria liquid prepared in step 1 into 10ml of bacteria liquid, and centrifuge. The rotating speed of the centrifuge is 10000 rpm, and the centrifugal time is 5min. After separation, remove the separation supernatant, add fresh YMA liquid medium to the separated rhizobium meliloti liquid medium to 10ml, mix well, and centrifuge again, add fresh YMA medium to the separated rhizobia meliloti bottom liquid YMA liquid medium to 10ml, mix well, centrifuge again, add fr...

specific Embodiment approach 3

[0038] Specific embodiment three: in the present embodiment, the preparation method of the rhizobia medatifa immobilized embedding granule dosage form is carried out according to the following steps:

[0039] Step 1, cultivating Rhizobium alfalfa: Utilize YMA liquid medium to cultivate Rhizobium alfalfa until the number of live bacteria reaches 1×10 9 More than one / ml, made into alfalfa rhizobium bacteria liquid;

[0040] Step 2, centrifugal separation of Rhizobium alfalfa bacteria liquid: the Rhizobium alfalfa bacteria liquid prepared in step 1 was divided into 10ml of bacteria liquid, and centrifuged. The rotating speed of the centrifuge was 5000 rpm, and the centrifugal time was 10min. After separation, remove the separation supernatant, add fresh YMA liquid medium to the separated rhizobium meliloti liquid medium to 10ml, mix well, and centrifuge again, add fresh YMA medium to the separated rhizobia meliloti bottom liquid YMA liquid medium to 10ml, mix well, centrifuge ag...

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Abstract

The invention discloses preparation and application of a novel alfalfa rhizobia immobilized embedded granule formation, and belongs to the field of agricultural microbe application. An immobilized cell technology is a method for immobilizing microbial cells in some special materials, preparing granules to form a limited space, enabling the thallus to keep biological activity in a certain time and realizing proliferation. At present, the alfalfa rhizobia is widely applied to the fields such as medicines, foods, fermentation industry, wastewater treatment and the like, preliminary study in a rhizobium japonicum embedding aspect is realized, while the preparation and application of the alfalfa rhizobia embedded immobilized granules is still a blank at present. According to the novel alfalfa rhizobia immobilized embedded granules developed in the invention, the alfalfa rhizobia is conveniently added by alfalfa cultivation enterprises, and the activity of fungicides is not lost in the compounding, processing, granulating and transportation and storage processes. Therefore, alfalfa rhizobia bacterial manure can be easily used by farmer households, and the main technical bottleneck difficulty of a high-tolerance high-activity alfalfa rhizobia seed embedding technique is broken.

Description

technical field [0001] The invention belongs to the application field of agricultural microorganisms, and in particular relates to the preparation and application of a new type of rhizobium meliloti embedment and immobilization granule dosage form. Background technique [0002] The symbiotic system of legumes and rhizobia is one of the most important biological nitrogen fixation systems in nature, and it is also the most widely used and the best economic and social benefits in agriculture. There are many types of rhizobia inoculants. Commonly used are common peat adsorption dosage form, liquid dosage form, agar slant culture dosage form, liquid mineral oil dosage form, freeze-dried dosage form and granule dosage form. Among them, the most commonly used one is the peat-absorbing bacterial agent, which is made by using peat to absorb the fermentation liquid. Because it contains a large amount of organic matter, microorganisms can proliferate at a suitable temperature, but th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/10C12N11/14C05G1/00C05F11/08C12R1/41
Inventor 高宇石杰孙宇峰刘淑霞王云云关向军徐浩然程皓苏丹
Owner DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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