76results about How to "No loss of activity" patented technology

The invention provides an orientated nano fiber bionic nerve conduit and a manufacturing method thereof. A nano fiber membrane with orientation degree larger than or equal to 75% is prepared by means of coaxial electrostatic spinning, and then is curved to form the nerve conduit, NGF (nerve growth factor) is added into fibers so that the bionic nerve conduit is combined with various factors promoting nerve regeneration, such as 'nano bionics', 'contact guiding', 'drug release' and the like, and the bionic nerve conduit can effective promote nerve regeneration as proved by animal experiments.

A protected active metal electrode and lithium-metal electrode and a device with the electrodes are provided. The protected active metal electrode includes an active metal substrate and a protection layer on a surface of the active metal substrate. The protection layer at least includes a metal thin film covering the surface of the active metal substrate and an electrically-conductive thin film covering a surface of the metal thin film. A material of the metal thin film is Ti, V, Cr, Zr, Nb, Mo, Hf, Ta, or W. A material of the electrically-conductive thin film is selected from nitride of a metal in the metal thin film, carbide of a metal in the metal thin film, a diamond-like carbon (DLC), and a combination thereof.

The invention discloses a preparation process of standard coprophilous fungi freeze-dried powder in transplantation therapy of coprophilous fungi. The preparation process comprises the following steps: (1) preparing standard coprophilous fungi liquid; (2) preparing a freeze-dried protecting agent; and (3) carrying out three-step-method freezing and vacuum drying, wherein the freeze-dried protecting agent is prepared by the steps of respectively adding 10%-20% of mycose, 5%-10% of glycerin and 0.02%-0.05% of vitamin C into sterile normal saline, uniformly mixing, filtering, and removing bacteria. According to the special freeze-dried protecting agent, the activity loss of the coprophilous fungi can be prevented, and the survival rate of the coprophilous fungi is increased; and by virtue ofa special freeze-drying procedure, the coprophilous fungi can rapidly enters a dormant state, so that the activity loss is avoided, and the stability of effective components is effectively protected.Experiments show that by utilizing the freeze-dried powder prepared by virtue of the preparation process, the activity of the coprophilous fungi in excrements is effectively maintained, and the survival rate of the coprophilous fungi is greatly increased.

The invention provides a sub-totipotent stem cell of a human placenta. The sub-totipotent stem cell of the human placenta is derived from stripped human placenta amnion, aiming at the characteristics of cells contained by an epithelial layer and a mesenchyme layer of the amnion, a step-by-step separation method is adopted, pollution of the sub-totipotent stem cell of the placenta by the mesenchyme layer of the amnion is reduced to the greatest extent, and the stemness of the sub-totipotent stem cell of the human placenta is improved effectively. The method has the characteristics of low cost, broad application prospect and capacity of supplying a large quantity of stem cell resources to clinic and research. The invention further provides a method for preparing the sub-totipotent stem cell of the human placenta from the human placenta amnion and constructing a stem cell bank.

The invention provides an ampelopsis grossedentata tea health-care compound beverage and preparation method thereof. The method comprises the following steps: by taking ampelopsis grossedentata tea, radix glycyrrhizae, flos lonicerae, radix ophiopogonis, bulbus lilii, herba lophatheri, folium mori, fructus lycii, fructus momordicae and rhizoma imperatae as raw materials, crushing the raw materials, leaching the crushed raw materials by adopting water extraction, alcohol extraction or combination of alcohol extraction with water extraction, and filtering to obtain leaching solution, and collecting filtrate to obtain ampelopsis grossedentata tea health-care compound beverage. The active efficacy of active ingredients in the prepared ampelopsis grossedentata tea health-care compound beverage can be effective guaranteed not to lose, the beverage has multiple health-care efficacies through the synergistic compatibility of multiple raw materials, the overall attribute of the beverage is mild or warm, so that the beverage is more applicable to drinking of the majority of consumers, the contents of each active ingredient in the beverage is within the daily maximum usage amount range, so that the safety of the beverage can be further guaranteed, the side effect due to excessive drinking can be avoided, and the beverage is healthier.

The invention discloses a culture solution for an in vitro efficient amplification of animal cells and an application of the culture solution. The culture solution consists of basal culture mediums, namely DMEM / F12, bFGF, EGF, sodium pyruvate, glutamine, sodium hydrogen carbonate and fetal calf serum. The culture solution disclosed by the invention has comparatively low content of animal serum, and is comparatively high in safety, simple and definite in formula components; the culture solution can avoid a possibility of different properties of the cultured cells due to a difference between culture medium batches, and is applicable to industrial production of cell products. Animal adherent cells cultured by the culture solution disclosed by the invention are comparatively strong in in-vitro amplification capacity and well maintain biological characteristics; and the culture solution is not only applicable to culture of adherent mesenchymal stem cells, but also suitable for culture of other fibroblast lines and other cell lines.

The invention discloses an in-situ preparation method of an electrode of an electrochemical capacitor. The method comprises the following steps: nickel foam is taken as a conductive substrate, and thesurface of the nickel foam is nanocrystallized through electrochemical cyclic voltammetry firstly; then the nickel foam is immersed into a mixed precursor solution containing nickel salt, cobalt salt, glucose and dicyandiamide, dried and pyrolyzed at high temperature, and hollow nanocarbon tubes (NiCoN-C / nano-G-Ni) supported on the nickel foam and doped with nickel-cobalt-nitrogen are obtained; NiCoN-C / nano-G-Ni is immersed into a manganese acetate solution at 40 DEG C and a potassium permanganate solution at 80 DEG C sequentially, dried and subjected to heat treatment at 250 DEG C, manganesedioxide is firmly inlaid in the surfaces of the hollow carbon tubes, and a manganese dioxide / nickel cobalt nitrogen-hollow carbon tube compound MnO2 / NiCoN-C / nano-G-Ni with the nickel foam as the substrate is obtained and can serve directly as an electrode material of an electrochemical supercapacitor. Troublesome common preparation steps for the electrode material are reduced, the problems of performance degradation of an active material and the like are solved, and the material has great practical application significance.

This invention provides a method for producing a composition comprising colloidal nanoparticles of metals including silver, gold, zinc, mercury, copper, palladium, platinum, or bismuth, by contacting a metal or metal compound with bacteria. An embodiment of the method comprises a step of incubating probiotic bacteria with an aqueous solution comprising at least 4 mM of a silver or gold salt. A resulting nanosilver-containing composition is useful as a highly efficient antimicrobial agent, for instance when impregnated onto a carrier, or an algicide agent or a herbicide agent.

The invention relates to the technical field of automobile exhaust gas purification, and concretely relates to an automobile exhaust gas treatment catalyst and a preparation method thereof. The catalyst comprises a carrier, and the carrier is sequentially coated with a first catalyst coating, a second catalyst coating and a third catalyst coating; the first catalyst coating is a lanthanum-modifiedoxygen storage material coating, and is formed by loading lanthanum on an oxygen storage material; the second catalyst coating is a barium-modified alumina supported active component coating, and iscomposed of a barium-modified gamma-Al2O3 supported active component; and the third catalyst coating is a coating material of meso-porous AcF micro-powder supported with and wrapping a precious metal.The catalyst has the advantages of high catalytic activity, great reduction of nitrogen oxides, carbon monoxide, hydrocarbon carbon and other series of pollutants in discharged automobile exhaust gas, good heat resistance, good stability, and improvement of the utilization rate of the precious metal.

The invention provides a mesenchymal stem cell cryopreservation liquid, a cryopreservation method, a preservation kit and a thawing method, and relates to the technical field of cell biology. The mesenchymal stem cell cryopreservation liquid comprises the following components with the following working concentrations: 25-35 ml / 100ml of a mesenchymal stem cell culture supernatant; 4-6 ml / 100ml of glycerol, 4-6 ml / 100ml of polyvinylpyrrolidone, 0.5-1.5 g / 100ml of polyvinyl alcohol, 0.5-2.5 g / 100mL of trehalose, 1-2.5 g / 100mL of hydroxyapatite nanoparticles, 0.5-1.5 mg / 100mL of all-trans retinoicacid and 8-12 ml / 100ml of a serum substitute; the mesenchymal stem cell culture supernatant is prepared by the following method: culturing mesenchymal stem cells at the convergence degree of 78-82% for 22-26 hours, filtering, and retaining a filtrate, thereby obtaining the mesenchymal stem cell culture supernatant. The cryopreservation liquid can effectively reduce damage to cells in cryopreservation and resuscitation processes and improve the activity of the resuscitated cells.

The invention provides a nanoparticle photocatalysis board, its preparation method and application thereof. The nanoparticle photocatalysis board comprises a substrate and a photosensitive nanoparticle coating that is cured on the substrate. The preparation method consists of: subjecting the substrate to ultrasonic cleaning and drying it in the air, and applying a light curing adhesive uniformly in a darkroom environment, then conducting drying treatment so as to obtain a light curing rubber board; spraying the photosensitive nanoparticles on the light curing rubber board uniformly, then placing it in an ultraviolet light environment for curing, thus obtaining the nanoparticle photocatalysis board, which can be applied in removing residual washing agents in washed clothes. The photocatalysis board provided in the invention has the advantages of low preparation cost, low particle desorption rate, current scour resistance, and no loss of the activity of the nanoparticles. The method of the invention is simple and operated at normal temperature, and it reduces the cost as well as improves production efficiency.

The invention provides an orientated nano fiber bionic nerve conduit and a manufacturing method thereof. A nano fiber membrane with orientation degree larger than or equal to 75% is prepared by means of coaxial electrostatic spinning, and then is curved to form the nerve conduit, NGF (nerve growth factor) is added into fibers so that the bionic nerve conduit is combined with various factors promoting nerve regeneration, such as 'nano bionics', 'contact guiding', 'drug release' and the like, and the bionic nerve conduit can effective promote nerve regeneration as proved by animal experiments.

The invention provides a preparation method of shinyleaf yellowhorn Fu tea which has the effects of lowering lipid, regulating blood pressure and lowering blood sugar. The method comprises the following steps of picking, shinyleaf yellowhorn flower pretreatment, shinyleaf yellowhorn tree leaf pretreatment, eurotium cristatum spore suspension preparation, blending of shinyleaf yellowhorn tree leaves, Fu tea and accessories, tribulus terrestris fruit juicing, fruit juice and fruit residue taking, adding of eurotium cristatum spore suspension and tribulus terrestris fruit juice in the process of stir-frying shinyleaf yellowhorn and Fu tea mixed materials and tribulus terrestris fruit residues for inoculation, tea pressing, fermenting, drying, inspecting and packing. The preparation method of the shinyleaf yellowhorn Fu tea has the advantages that the shinyleaf yellowhorn and Fu tea serve as the raw materials, and the effects of lowering the lipid, regulating the blood pressure and lowering the blood sugar are achieved; a unique fermentation process is adopted, and eurotium cristatum can grow out on the surface and in the interior of the Fu tea; in the whole technological process, it is guaranteed that the original activity of the shinyleaf yellowhorn tree leaves is not lost, and the whole fermentation period is shortened; the prepared Fu tea is good in color and luster, mellow in taste and capable of being stored for a long time.

The invention discloses a preparation method of a calcium peroxide microcapsule. The method comprises the following steps: 1, dissolving a polymer in dichloromethane to obtain a polymer solution, wherein the polymer is ethylcellulose or a polylactic acid and ethylcellulose mixture; 2, adding calcium peroxide to the polymer solution obtained in step 1, and stirring for dispersion; and 3, adding petroleum ether to a reaction system obtained in step 2 in a dropwise manner, and stirring for dispersion to obtain the calcium peroxide microcapsule. The calcium peroxide microcapsule prepared through the preparation method has the advantages of controllable dimension, regular profile, and controllable drug release rate, and a pulvis is convenient for oral local application; and the preparation method has the advantages of simplicity, feasibility, high preparation efficiency, and convenience for large scale application.

The invention relates to a preparation and verification method of a Chlorella protein molecule, which comprises the steps of: suspending Chlorella mud in pH 7.0 0.001mol / L phosphate buffer solution (PBS), and repeatedly freezing and thawing three times; ultrasonically crushing in an ice bath for 9min; centrifuging at the temperature of 4 DEG C and 12000r / min for 30min, collecting supernatant and adding ammonium sulfate with saturation degree of 30%; standing at the temperature of 4 DEG C for 24h, and centrifuging at the temperature of 4 DEG C and 12000r / min for 30min; and concentrating the supernatant with an ultrafiltration cup to 200mL, dissolving precipitate with little PBS, placing in a dialysis membrane, dialyzing in pH 7.0 0.001mol / L PBS under stirring for 2d, and embedding with polyethylene glycol-6000 to 60mL. By the adoption of the method to separate the purified protein molecule of Chlorella, the protein is released from the tissue or cell, the original native state is kept, and the activity is not lost, so that the specific chemical structure of the protein molecule can be accurately determined.

The invention relates to a Pichia pastoris engineering strain GS-TA-LGH expressing a thermoascus aurantiacus var. levisporus thermostable lipase gene lgh. According to the invention, the lipase gene lgh is obtained from thermoascus aurantiacus var. levisporus through RT-PCR (reverse transcription-polymerase chain reaction), RACE (rapid-amplification of cDNA (complementary deoxyribonucleic acid) ends) and other methods, an expression vector pPIC9K / lgh is constructed and introduced into Pichia pastoris GS115, and then the Pichia pastoris engineering strain GS-TA-LGH expressing the lipase is screened out from the Pichia pastoris GS115. The enzyme activity of the lipase of the engineering strain can achieve 19.92U / mg, when heat insulation is performed on the enzyme at the temperature of 50 DEG C for 60 minutes, the loss of the activity can be avoided; and when the heat insulation is performed at the temperature of 60 DEG C for 60 minutes, the enzyme activity can still achieve 66%. The Pichia pastoris engineering strain GS-TA-LGH has higher thermal stability and has economical value and social value as the strain for producing the thermostable lipase.

The invention discloses a preparation method of a human blood coagulation factor VIII and a human blood coagulation factor VIII product and relates to the field of blood products. The preparation method of the human blood coagulation factor VIII comprises the following steps: dissolving a cryoprecipitate with a 0.015-0.025mol / L tromethamine solution in a mass ratio of (3-5): 1 by reasonably processing the cryoprecipitate; and carrying out separation and purification by means of a polyethylene glycol precipitating method combined with ion exchange chromatography, wherein the recovery ratio of the human blood coagulation factor VIII and the specific activity of a final product can be improved effectively, the yield reaches 180-240IU / L plasma, and the specific activity is not lower than 100IU / mg proteins. The prepared human blood coagulation factor VIII product is rich in vWF factors and the proportion of the vWF factors and the human blood coagulation factor VIII is close to 1: 1. Besides treating hemophiliac A, the human blood coagulation factor VIII can be also used for treating patients with angiohemophilia, and the human blood coagulation factor VIII has good stability and heat resistance.

The invention relates to an electrophoretic separation device and a technology used for laboratorial and large-scale separated and purified biological samples. The invention mainly relates to the electrophoretic separation device which is a device capable of being used for performing the non-denaturing electrophoretic preparation technology, and a method required for completing the electrophoretic preparation technology by the device. The device mainly comprises a cooling chamber which is provided with a good heat exchange structure, a vertical electrophoretic plate with non-denaturing polyacrylamide gel arranged in the cooling chamber, a concentrating and collecting chamber which is used for sample collection, and an electrophoretic cell. The technology mainly comprises screening of collection time of purified proteins and a method for collecting the purified proteins. The device and the technology have the characteristics of large processing capacity, high resolution, convenient operation and so on, can be used for preparing laboratorial and large-scale biological products and biological medicines, and have considerable economic benefit and social benefit.

The invention relates to a pyridine type chiral Cu(II)-Salen ligand metal organic framework crystal material as well as a preparation method and application thereof. The material has the following chemical formula: {[Zn2(L)(BPDC)2].DMF.5H2O}n, wherein L is (R,R)-N,N'-bis(5-(4-pyridyl) diyl-2-hydroxyacetophenone)-1,2-diphenylethylenediamine copper (II), BPDC is a 4,4'-biphenyl dicarboxylate divalent anion, and n is the degree of polymerization. The metal organic framework crystal material provided by the invention adopts a solvothermal synthesis method, and is simple to operate, low in cost, high in yield and easy for large-scale industrial production. The prepared metal organic framework crystal material has a relatively high specific surface area (BET specific surface area is 752 m<2> / g),and the adsorption capacities of CO2 and N2 under 1atm and 273 K are 3.47 mmol / g and 0.57 mmol / g respectively. TEMPO is used as an additive, selective oxidation of benzyl alcohol is catalyzed in a water phase to generate benzaldehyde, the yield reaches 99%, the catalyst is recycled for five times with almost no activity loss, and the catalyst is a good heterogeneous catalyst.

The invention provides a method and device for spraying thermal fog carried biological pesticide, so that biological pesticide liquor is thermally fogged to rise up, spread and suspend over the prevention space while not losing the activity of the biological pesticide. The method for spraying biological pesticide is as follows: adding the oil-based suspension of the biological pesticide into the following solvent to prepare hot fogging concentrate, and then spraying the hot fogging concentrate into the spraying tube of a fog machine to be fogged and sprayed out. The temperature of the fogged hot fogging concentrate at the nozzle is 300 DEG C + / - 500 DEG C; and, in the solvent, the ratio of glycerol to water by volume is 55-75:25-45. The device for spraying biological pesticide includes a fog machine, the fog machine has a spraying tube, a nozzle is arranged on the spraying tube, the nozzle is communicated with a pesticide tank, and the pesticide tank is communicated with a gas pressure tube; and the pesticide tank is filled with thermal fogging concentrate mixed from oil-base suspension of the biological pesticide, glycerol and water.

The present invention relates to a method for preparing sericin from tussah silk degumming industrial wastewater. According to the present invention, tussah silk industrial wastewater is adopted as a raw material, the total content of the protein in the solution is detected by using a Folin-phenol colorimetry method, the color and the presence of the precipitate of the tussah silk industrial wastewater are observed, the pH value is measured, the sericin in the solution is subjected to crude extraction by using a salting out method, a large amount of salt ions in the protein solution are removed through dialysis operation, and the sericin in the solution is further extracted through an organic precipitation method to improve the protein purity in the solution so as to achieve the sericin preparation from the tussah silk production industrial wastewater; and with the method, the environmental pollution can be prevented, the sewage treatment problem is solved, and the extracted sericin can be used in the fields of cosmetics, food, fiber and textiles, medicine, biological materials and the like, and has important significance in the research of the modern new industry.

The invention discloses a colon-targeted oral probiotic microcapsule and a preparation method and application thereof, and belongs to the field of biomedical engineering. A core of the microcapsule is a probiotics-containing calcium alginate gel core, and a shell of the microcapsule is coated with multiple layers of chitosan-starch. The microcapsule has a compact coating structure, can prevent probiotics from being eroded by gastric acid and cholate and can be triggered by trypsin, so that a carrier is efficiently disintegrated in a fixed point at the colon, and a targeting property is excellent; a chitosan coating on the periphery of the carrier endows the probiotics with relatively high intestinal mucosa adhesion; and oligosaccharides generated after starch on the periphery of the carrier is digested can serve as prebiotics substances to promote growth of the probiotics after fixed-point adhesion. Therefore, the oral probiotic micro-capsule provided by the invention is an oral probiotic micro-capsule that is resistant to both the gastric acid and the cholate, has a clear release site and high release degree, improves thallus adhesion and promotes the growth of the probiotics.

The invention discloses a fertilizer reduction synergist combining an amino acid polymer and biological bacteria. The fertilizer reduction synergist is prepared from the following raw materials in parts by weight: 30-50 g of an amino acid polymer, 50-70 g of bacillus licheniformis, 50-70 g of bacillus subtilis and 4000-5000 g of a base material. The fertilizer reduction synergist has the followingadvantages: 1, the use amount of chemical fertilizers per mu is reduced by 35-50%, so that the use amount of the chemical fertilizers is effectively reduced, cost is saved, saline-alkali soil hardening caused by excessive use of chemical fertilizers is relieved, and influence of excessive chemical fertilizers on soil quality is avoided; 2, the fertilizer reduction synergist has the advantages that soil is loosened, slow-release synergism of fertilizers is achieved, phosphorus is activated, water retention and drought resistance are achieved, straw can be degraded, crop diseases are reduced, the use amount of pesticides is reduced, quality of agricultural products is improved, and condition guarantees are provided for pollution-free products; and 3, activity and performance of the amino acid polymer and the two biological bacteria can be prevented from being lost, and the two biological bacteria can be promoted to generate more amino acid polymers through cross-linking, so that the action of the amino acid polymers is increased, and the action time of the amino acid polymers in soil is prolonged.

The invention relates to the field of compound synthesis, in particular to a method for preparing chiral organoboron compound through catalysis of functionalized chitosan support copper, and further relates to application of the method for preparing the chiral organoboron compound through catalysis of functionalized chitosan support copper in synthesizing a delta-hydroxyl compound and diabetes treatment drug molecules. The functionalized chitosan support copper is adopted as a catalyst, bisdiboron is adopted as a reaction reagent, water is adopted as a solvent, sodium perborate tetrahydrate isadopted as an oxidant, boron addition reactions are selectively performed for substrates with different substituent groups respectively, and furthermore, the delta-hydroxyl compound is prepared through a continuous oxidation reaction.

The invention relates to a method for preparing sulfuric acid by catalytically oxidizing low-concentration sulfur dioxide in baking flue gas. The method comprises the following steps of (1) introducing baking flue gas into a spray absorption tower, and spraying water onto the tower bottom of the spray absorption tower for dedusting and cooling to obtain a cooled spray liquid; (2) filling cooled and dedusted baking flue gas into a wet electron exchange resin layer loaded on the spray absorbing tower, oxidizing an electron exchange resin into an oxidization type resin with oxygen in the baking flue gas, reducing the oxidation type resin into a reduction type resin by using sulfur dioxide in the baking flue gas, oxidizing the sulfur dioxide into sulfur trioxide, and spraying with the spraying absorbing tower to obtain a dilute sulfuric acid; and (3) finally exhausting the baking flue gas out of an upper layer demister layer of the spray absorbing tower. The method has the beneficial effects that scaling and blocking are avoided; the desulfurizing efficiency is high; the investment is small; and nearly zero-cost running is realized, and great social benefit and economic benefit can be created.

The invention discloses a method for preparing quinazolone and derivatives thereof by using a chitosan-loaded copper catalyst, which comprises the following steps of replacing residual gas in a reaction container with inert gas, adding a catalytic amount of copper ion-loaded chitosan catalyst, substituted 2-halogenated benzoic acid, substituted amidine hydrochloride, inorganic alkali and a mixed solvent into the reaction container, and heating for reaction, after the reaction time is 2-18 hours, extracting the product by using ethyl acetate, filtering and recovering the copper ion-loaded chitosan catalyst, concentrating the filtrate under reduced pressure, and purifying the product by column chromatography. The method has the advantages of low catalyst dosage, recoverability, easy separation after reaction, no metal residue, simple post-treatment, and suitableness for large-scale production.

The invention relates to a high-polymerization Salen cobalt catalyst as well as a preparation method and application thereof. The catalyst has a structural general formula shown in the specification. The catalyst is stable and has the advantages of being small in dosage, high in resolution efficiency, easy to recycle, simple in synthesis method, low in price of raw materials used for synthesis, low in production cost and convenient for large-scale industrial application in the application of hydrolysis kinetic resolution of various different terminal epoxy compounds .

The invention provides a method of extracting protein from potato starch processing waste liquid. The method includes the following steps: 1) centrifugally filtering the potato starch processing waste liquid to obtain primary protein liquid; 2) adding ethanol, saturation degree being 95-98%, to the protein liquid, and stirring and soaking the protein liquid to prepare a precipitate; 3) adding deionized water to the precipitate and purifying the precipitate with macroporous resin, wherein the liquid is eluted by 90-94% ethanol and then is eluted by sterilized clear water, thereby preparing an eluent; and 4) dialyzing and drying the eluent to obtain a protein extract. The method is convenient, wherein the activity of the extracted protein is not lost, and functions of proteins are improved. The purity of the extracted proteins is high.