76results about How to "No loss of activity" patented technology

The invention discloses a preparation process of standard coprophilous fungi freeze-dried powder in transplantation therapy of coprophilous fungi. The preparation process comprises the following steps: (1) preparing standard coprophilous fungi liquid; (2) preparing a freeze-dried protecting agent; and (3) carrying out three-step-method freezing and vacuum drying, wherein the freeze-dried protecting agent is prepared by the steps of respectively adding 10%-20% of mycose, 5%-10% of glycerin and 0.02%-0.05% of vitamin C into sterile normal saline, uniformly mixing, filtering, and removing bacteria. According to the special freeze-dried protecting agent, the activity loss of the coprophilous fungi can be prevented, and the survival rate of the coprophilous fungi is increased; and by virtue ofa special freeze-drying procedure, the coprophilous fungi can rapidly enters a dormant state, so that the activity loss is avoided, and the stability of effective components is effectively protected.Experiments show that by utilizing the freeze-dried powder prepared by virtue of the preparation process, the activity of the coprophilous fungi in excrements is effectively maintained, and the survival rate of the coprophilous fungi is greatly increased.

The invention provides an ampelopsis grossedentata tea health-care compound beverage and preparation method thereof. The method comprises the following steps: by taking ampelopsis grossedentata tea, radix glycyrrhizae, flos lonicerae, radix ophiopogonis, bulbus lilii, herba lophatheri, folium mori, fructus lycii, fructus momordicae and rhizoma imperatae as raw materials, crushing the raw materials, leaching the crushed raw materials by adopting water extraction, alcohol extraction or combination of alcohol extraction with water extraction, and filtering to obtain leaching solution, and collecting filtrate to obtain ampelopsis grossedentata tea health-care compound beverage. The active efficacy of active ingredients in the prepared ampelopsis grossedentata tea health-care compound beverage can be effective guaranteed not to lose, the beverage has multiple health-care efficacies through the synergistic compatibility of multiple raw materials, the overall attribute of the beverage is mild or warm, so that the beverage is more applicable to drinking of the majority of consumers, the contents of each active ingredient in the beverage is within the daily maximum usage amount range, so that the safety of the beverage can be further guaranteed, the side effect due to excessive drinking can be avoided, and the beverage is healthier.

The invention discloses a culture solution for an in vitro efficient amplification of animal cells and an application of the culture solution. The culture solution consists of basal culture mediums, namely DMEM/F12, bFGF, EGF, sodium pyruvate, glutamine, sodium hydrogen carbonate and fetal calf serum. The culture solution disclosed by the invention has comparatively low content of animal serum, and is comparatively high in safety, simple and definite in formula components; the culture solution can avoid a possibility of different properties of the cultured cells due to a difference between culture medium batches, and is applicable to industrial production of cell products. Animal adherent cells cultured by the culture solution disclosed by the invention are comparatively strong in in-vitro amplification capacity and well maintain biological characteristics; and the culture solution is not only applicable to culture of adherent mesenchymal stem cells, but also suitable for culture of other fibroblast lines and other cell lines.

The invention discloses an in-situ preparation method of an electrode of an electrochemical capacitor. The method comprises the following steps: nickel foam is taken as a conductive substrate, and thesurface of the nickel foam is nanocrystallized through electrochemical cyclic voltammetry firstly; then the nickel foam is immersed into a mixed precursor solution containing nickel salt, cobalt salt, glucose and dicyandiamide, dried and pyrolyzed at high temperature, and hollow nanocarbon tubes (NiCoN-C/nano-G-Ni) supported on the nickel foam and doped with nickel-cobalt-nitrogen are obtained; NiCoN-C/nano-G-Ni is immersed into a manganese acetate solution at 40 DEG C and a potassium permanganate solution at 80 DEG C sequentially, dried and subjected to heat treatment at 250 DEG C, manganesedioxide is firmly inlaid in the surfaces of the hollow carbon tubes, and a manganese dioxide/nickel cobalt nitrogen-hollow carbon tube compound MnO2/NiCoN-C/nano-G-Ni with the nickel foam as the substrate is obtained and can serve directly as an electrode material of an electrochemical supercapacitor. Troublesome common preparation steps for the electrode material are reduced, the problems of performance degradation of an active material and the like are solved, and the material has great practical application significance.

This invention provides a method for producing a composition comprising colloidal nanoparticles of metals including silver, gold, zinc, mercury, copper, palladium, platinum, or bismuth, by contacting a metal or metal compound with bacteria. An embodiment of the method comprises a step of incubating probiotic bacteria with an aqueous solution comprising at least 4 mM of a silver or gold salt. A resulting nanosilver-containing composition is useful as a highly efficient antimicrobial agent, for instance when impregnated onto a carrier, or an algicide agent or a herbicide agent.

The invention provides a preparation method of shinyleaf yellowhorn Fu tea which has the effects of lowering lipid, regulating blood pressure and lowering blood sugar. The method comprises the following steps of picking, shinyleaf yellowhorn flower pretreatment, shinyleaf yellowhorn tree leaf pretreatment, eurotium cristatum spore suspension preparation, blending of shinyleaf yellowhorn tree leaves, Fu tea and accessories, tribulus terrestris fruit juicing, fruit juice and fruit residue taking, adding of eurotium cristatum spore suspension and tribulus terrestris fruit juice in the process of stir-frying shinyleaf yellowhorn and Fu tea mixed materials and tribulus terrestris fruit residues for inoculation, tea pressing, fermenting, drying, inspecting and packing. The preparation method of the shinyleaf yellowhorn Fu tea has the advantages that the shinyleaf yellowhorn and Fu tea serve as the raw materials, and the effects of lowering the lipid, regulating the blood pressure and lowering the blood sugar are achieved; a unique fermentation process is adopted, and eurotium cristatum can grow out on the surface and in the interior of the Fu tea; in the whole technological process, it is guaranteed that the original activity of the shinyleaf yellowhorn tree leaves is not lost, and the whole fermentation period is shortened; the prepared Fu tea is good in color and luster, mellow in taste and capable of being stored for a long time.

The invention relates to a Pichia pastoris engineering strain GS-TA-LGH expressing a thermoascus aurantiacus var. levisporus thermostable lipase gene lgh. According to the invention, the lipase gene lgh is obtained from thermoascus aurantiacus var. levisporus through RT-PCR (reverse transcription-polymerase chain reaction), RACE (rapid-amplification of cDNA (complementary deoxyribonucleic acid) ends) and other methods, an expression vector pPIC9K/lgh is constructed and introduced into Pichia pastoris GS115, and then the Pichia pastoris engineering strain GS-TA-LGH expressing the lipase is screened out from the Pichia pastoris GS115. The enzyme activity of the lipase of the engineering strain can achieve 19.92U/mg, when heat insulation is performed on the enzyme at the temperature of 50 DEG C for 60 minutes, the loss of the activity can be avoided; and when the heat insulation is performed at the temperature of 60 DEG C for 60 minutes, the enzyme activity can still achieve 66%. The Pichia pastoris engineering strain GS-TA-LGH has higher thermal stability and has economical value and social value as the strain for producing the thermostable lipase.

The invention discloses a preparation method of a human blood coagulation factor VIII and a human blood coagulation factor VIII product and relates to the field of blood products. The preparation method of the human blood coagulation factor VIII comprises the following steps: dissolving a cryoprecipitate with a 0.015-0.025mol/L tromethamine solution in a mass ratio of (3-5): 1 by reasonably processing the cryoprecipitate; and carrying out separation and purification by means of a polyethylene glycol precipitating method combined with ion exchange chromatography, wherein the recovery ratio of the human blood coagulation factor VIII and the specific activity of a final product can be improved effectively, the yield reaches 180-240IU/L plasma, and the specific activity is not lower than 100IU/mg proteins. The prepared human blood coagulation factor VIII product is rich in vWF factors and the proportion of the vWF factors and the human blood coagulation factor VIII is close to 1: 1. Besides treating hemophiliac A, the human blood coagulation factor VIII can be also used for treating patients with angiohemophilia, and the human blood coagulation factor VIII has good stability and heat resistance.

The invention relates to an electrophoretic separation device and a technology used for laboratorial and large-scale separated and purified biological samples. The invention mainly relates to the electrophoretic separation device which is a device capable of being used for performing the non-denaturing electrophoretic preparation technology, and a method required for completing the electrophoretic preparation technology by the device. The device mainly comprises a cooling chamber which is provided with a good heat exchange structure, a vertical electrophoretic plate with non-denaturing polyacrylamide gel arranged in the cooling chamber, a concentrating and collecting chamber which is used for sample collection, and an electrophoretic cell. The technology mainly comprises screening of collection time of purified proteins and a method for collecting the purified proteins. The device and the technology have the characteristics of large processing capacity, high resolution, convenient operation and so on, can be used for preparing laboratorial and large-scale biological products and biological medicines, and have considerable economic benefit and social benefit.

The invention provides a method and device for spraying thermal fog carried biological pesticide, so that biological pesticide liquor is thermally fogged to rise up, spread and suspend over the prevention space while not losing the activity of the biological pesticide. The method for spraying biological pesticide is as follows: adding the oil-based suspension of the biological pesticide into the following solvent to prepare hot fogging concentrate, and then spraying the hot fogging concentrate into the spraying tube of a fog machine to be fogged and sprayed out. The temperature of the fogged hot fogging concentrate at the nozzle is 300 DEG C +/- 500 DEG C; and, in the solvent, the ratio of glycerol to water by volume is 55-75:25-45. The device for spraying biological pesticide includes a fog machine, the fog machine has a spraying tube, a nozzle is arranged on the spraying tube, the nozzle is communicated with a pesticide tank, and the pesticide tank is communicated with a gas pressure tube; and the pesticide tank is filled with thermal fogging concentrate mixed from oil-base suspension of the biological pesticide, glycerol and water.

The present invention relates to a method for preparing sericin from tussah silk degumming industrial wastewater. According to the present invention, tussah silk industrial wastewater is adopted as a raw material, the total content of the protein in the solution is detected by using a Folin-phenol colorimetry method, the color and the presence of the precipitate of the tussah silk industrial wastewater are observed, the pH value is measured, the sericin in the solution is subjected to crude extraction by using a salting out method, a large amount of salt ions in the protein solution are removed through dialysis operation, and the sericin in the solution is further extracted through an organic precipitation method to improve the protein purity in the solution so as to achieve the sericin preparation from the tussah silk production industrial wastewater; and with the method, the environmental pollution can be prevented, the sewage treatment problem is solved, and the extracted sericin can be used in the fields of cosmetics, food, fiber and textiles, medicine, biological materials and the like, and has important significance in the research of the modern new industry.

The invention relates to a method for preparing sulfuric acid by catalytically oxidizing low-concentration sulfur dioxide in baking flue gas. The method comprises the following steps of (1) introducing baking flue gas into a spray absorption tower, and spraying water onto the tower bottom of the spray absorption tower for dedusting and cooling to obtain a cooled spray liquid; (2) filling cooled and dedusted baking flue gas into a wet electron exchange resin layer loaded on the spray absorbing tower, oxidizing an electron exchange resin into an oxidization type resin with oxygen in the baking flue gas, reducing the oxidation type resin into a reduction type resin by using sulfur dioxide in the baking flue gas, oxidizing the sulfur dioxide into sulfur trioxide, and spraying with the spraying absorbing tower to obtain a dilute sulfuric acid; and (3) finally exhausting the baking flue gas out of an upper layer demister layer of the spray absorbing tower. The method has the beneficial effects that scaling and blocking are avoided; the desulfurizing efficiency is high; the investment is small; and nearly zero-cost running is realized, and great social benefit and economic benefit can be created.

The invention discloses a method for preparing quinazolone and derivatives thereof by using a chitosan-loaded copper catalyst, which comprises the following steps of replacing residual gas in a reaction container with inert gas, adding a catalytic amount of copper ion-loaded chitosan catalyst, substituted 2-halogenated benzoic acid, substituted amidine hydrochloride, inorganic alkali and a mixed solvent into the reaction container, and heating for reaction, after the reaction time is 2-18 hours, extracting the product by using ethyl acetate, filtering and recovering the copper ion-loaded chitosan catalyst, concentrating the filtrate under reduced pressure, and purifying the product by column chromatography. The method has the advantages of low catalyst dosage, recoverability, easy separation after reaction, no metal residue, simple post-treatment, and suitableness for large-scale production.