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Method for producing metal nanoparticles

A metal and colloidal metal technology, applied in the field of preparation of silver or gold nanoparticles, disinfection products, silver or gold nanoparticles to improve their antimicrobial efficiency, can solve the problems of insufficient bactericidal activity of silver nanoparticles, long incubation time, etc., to achieve Reproducible controllable size and distribution, reduced demand, cost-effective effects

Inactive Publication Date: 2013-03-20
JANSSEN PHARMA NV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0015] Drawbacks of the above methods are the need for high temperatures, acidic pH and / or long incubation times, or insufficient bactericidal activity of the silver nanoparticles produced thereby

Method used

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Examples

Experimental program
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Embodiment approach

[0061] According to another embodiment of the present invention, the method comprises the step of incubating the probiotic bacteria (as defined hereinbefore) with an aqueous solution containing at least 4 mM gold salt, which aqueous solution may also contain a suitable amount of alkali metal hydroxide, such as but Not limited to sodium hydroxide or potassium hydroxide in the absence of ammonia and / or ammonium salts.

[0062] A suitable alkali metal hydroxide such as, but not limited to, sodium hydroxide or potassium hydroxide, may be added to the aqueous incubation solution to a concentration of up to about 1M. Preferably, the incubation is performed at a pH of at least 8, such as in the range of about 8 to about 12, or in a more specific embodiment from about 8.5 to about 11.

[0063]According to one embodiment of the present invention, the ratio of the weight of silver or gold to the dry cell weight of probiotic bacteria (hereinafter abbreviated as CDW) is at least about 0.0...

Embodiment 1

[0101] Embodiment 1--the preparation of nano silver

[0102] A culture of Lactobacillus fermentum Beijerinck 1901AL (ATCC 11976, LMG 8900, from the intestinal tract of 8-day-old breastfed infants) was prepared in MRS broth (purchased from Oxoid, Basingstoke, United Kingdom). ) and cultured at 37°C for 15 hours under moderate and microaerobic conditions. Collect cells from MRS by centrifugation at 3,000 g at 15°C for 10 minutes, wash twice with milliQ water, and then resuspend in milliQ water to obtain a final optical density at 600 nm (OD 600 ) is 1.5. Add 1N sodium hydroxide mother solution to the cell suspension, such as the final concentration of 0.05N NaOH and 0.10N NaOH.

[0103] Prepare 425mg AgNO in 50mL milliQ water 3 and 225mg NH 4 Cl in Ag(I) mother liquor. 1 volume of this Ag(I) stock solution was added to 10 volumes of cell suspension containing 0.05 and 0.10 N NaOH, respectively. These mixtures were incubated under visible light at 25°C with gentle agitati...

Embodiment 2

[0104] Embodiment 2-nano silver XRD analysis

[0105] After the biological substance with silver particles obtained in Example 1 was further dried at 30°C, X- Ray Diffraction (XRD). X-rays were generated by a copper X-ray tube with a power of 1.6kW (40kV, 40mA). Measurements were made between 25-90 degrees 2Θ with a "tep time" of 1.6 seconds in steps of 0.02 degrees. The resulting spectrum (not shown) indicated the presence of metallic silver and sodium oxide X-ray diffraction patterns. The latter is the residue of sodium hydroxide used to make nanosilver.

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Abstract

This invention provides a method for producing a composition comprising colloidal nanoparticles of metals including silver, gold, zinc, mercury, copper, palladium, platinum, or bismuth, by contacting a metal or metal compound with bacteria. An embodiment of the method comprises a step of incubating probiotic bacteria with an aqueous solution comprising at least 4 mM of a silver or gold salt. A resulting nanosilver-containing composition is useful as a highly efficient antimicrobial agent, for instance when impregnated onto a carrier, or an algicide agent or a herbicide agent.

Description

[0001] This application is a divisional application of the international application PCT / EP2007 / 006145, the international application date is July 5, 2007, the Chinese national phase application number is 200780025198.7, and the title is "method for preparing metal nanoparticles". field of invention [0002] The present invention relates to the preparation of colloidal metal compounds on bacterial membranes. The present invention also relates to methods of preparing silver or gold nanoparticles by biological processes. Specifically, the present invention relates to the use of probiotics, such as Lactobacillus, to produce metal nanoprecipitates, especially silver or gold nanoparticles, under specific conditions to increase their antimicrobial efficiency. The present invention also relates to a disinfection product comprising a carrier impregnated with the composition containing the colloidal nano silver or nano gold prepared by the method. Background of the invention [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/225
CPCY02A50/30
Inventor W·德温特T·韦科特恩W·韦斯特雷特
Owner JANSSEN PHARMA NV
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