Preparation process of standard coprophilous fungi freeze-dried powder in transplantation therapy of coprophilous fungi

Abstract

The invention discloses a preparation process of standard coprophilous fungi freeze-dried powder in transplantation therapy of coprophilous fungi. The preparation process comprises the following steps: (1) preparing standard coprophilous fungi liquid; (2) preparing a freeze-dried protecting agent; and (3) carrying out three-step-method freezing and vacuum drying, wherein the freeze-dried protecting agent is prepared by the steps of respectively adding 10%-20% of mycose, 5%-10% of glycerin and 0.02%-0.05% of vitamin C into sterile normal saline, uniformly mixing, filtering, and removing bacteria. According to the special freeze-dried protecting agent, the activity loss of the coprophilous fungi can be prevented, and the survival rate of the coprophilous fungi is increased; and by virtue ofa special freeze-drying procedure, the coprophilous fungi can rapidly enters a dormant state, so that the activity loss is avoided, and the stability of effective components is effectively protected.Experiments show that by utilizing the freeze-dried powder prepared by virtue of the preparation process, the activity of the coprophilous fungi in excrements is effectively maintained, and the survival rate of the coprophilous fungi is greatly increased.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a preparation process of standard fecal bacteria freeze-dried powder in fecal transplantation treatment. Background technique [0002] Fecal microbiota transplantation (FMT) began at least in the 4th century AD in the history of world medicine, but it has not achieved unprecedented rapid development until recent years. In 2013, FMT was first included in the US medical guidelines for recurrent Clostridium difficile infection [0003] (clostridium difficile infection, CDI) treatment. At the same time, its use in the treatment of inflammatory bowel disease, irritable bowel syndrome, metabolic syndrome and other intestinal and intestinal diseases at home and abroad has also attracted widespread attention. Currently, fecal microbiota transplantation is performed clinically by taking fresh feces from donors to prepare a standard fecal microbiota solution for treatment. This fresh f...

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Problems solved by technology

[0005] The purpose of the present invention is to solve the defect that the activity of the fecal bacteria freeze-dried powder is not high at present, and to invent a preparation process of the fecal bacteria freeze-dried powder, which can effectively maintain the activity of the fecal bacteria

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