Preparation process of standard coprophilous fungi freeze-dried powder in transplantation therapy of coprophilous fungi

A preparation process, freeze-dried powder technology, applied in the medical field, can solve the problem of low activity of fecal bacteria freeze-dried powder, and achieve the effects of improving the survival rate of fecal bacteria, protecting stability, and counting accurately

Inactive Publication Date: 2018-01-12
南京益恒寿生命科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the defect that the activity of the fecal bacteria freeze-dried powder is not high at present, and to invent a preparation process of the fecal bacteria freeze-dried powder, which can effectively maintain the activity of the fecal bacteria

Method used

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  • Preparation process of standard coprophilous fungi freeze-dried powder in transplantation therapy of coprophilous fungi

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Experimental program
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Effect test

Embodiment 1

[0026] A preparation process of standard fecal bacteria freeze-dried powder in the treatment of feces transplantation, comprising the following steps:

[0027] (1) Preparation of standard fecal liquid: 5 qualified donors (aged 25-35 years old, healthy males) were screened, 50 g of feces were collected aseptically, homogenized, filtered through gauze, centrifuged, and discarded. The supernatant liquid obtains the sludge sediment of fecal bacteria.

[0028] (2) Preparation of lyoprotectant: add 15% trehalose, 7% glycerin and 0.05% vitamin C to 100 mL sterile normal saline, mix well, and filter to sterilize.

[0029] (3) Three-step method of freezing and vacuum drying: add the freeze-drying protective agent into the fecal bacteria slurry, vortex and mix well, take 1 mL of the bacterial liquid for bifidobacteria culture and count, and put the remaining liquid into a freeze-drying tube. Then place the freeze-drying tube in the freeze-dryer and set the freeze-drying program: quickl...

Embodiment 1 5

[0034] The fecal bacteria count result of five groups of parallel experiments of embodiment 1 is shown in Table 1:

[0035] Table 1 The total number of bifidobacteria ( lg10 n )

[0036]

[0037] The average total number of viable bifidobacteria in the sample before freeze-drying was 6.54±0.31log 10 cfu, the average number of viable bifidobacteria after three months of freeze-drying storage is 6.02±0.51log 10 cfu, the survival rate was 92%.

[0038] The result proves that the freeze-dried powder prepared by the process provided by the invention effectively maintains the activity of fecal bacteria and greatly improves the survival rate.

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Abstract

The invention discloses a preparation process of standard coprophilous fungi freeze-dried powder in transplantation therapy of coprophilous fungi. The preparation process comprises the following steps: (1) preparing standard coprophilous fungi liquid; (2) preparing a freeze-dried protecting agent; and (3) carrying out three-step-method freezing and vacuum drying, wherein the freeze-dried protecting agent is prepared by the steps of respectively adding 10%-20% of mycose, 5%-10% of glycerin and 0.02%-0.05% of vitamin C into sterile normal saline, uniformly mixing, filtering, and removing bacteria. According to the special freeze-dried protecting agent, the activity loss of the coprophilous fungi can be prevented, and the survival rate of the coprophilous fungi is increased; and by virtue ofa special freeze-drying procedure, the coprophilous fungi can rapidly enters a dormant state, so that the activity loss is avoided, and the stability of effective components is effectively protected.Experiments show that by utilizing the freeze-dried powder prepared by virtue of the preparation process, the activity of the coprophilous fungi in excrements is effectively maintained, and the survival rate of the coprophilous fungi is greatly increased.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a preparation process of standard fecal bacteria freeze-dried powder in fecal transplantation treatment. Background technique [0002] Fecal microbiota transplantation (FMT) began at least in the 4th century AD in the history of world medicine, but it has not achieved unprecedented rapid development until recent years. In 2013, FMT was first included in the US medical guidelines for recurrent Clostridium difficile infection [0003] (clostridium difficile infection, CDI) treatment. At the same time, its use in the treatment of inflammatory bowel disease, irritable bowel syndrome, metabolic syndrome and other intestinal and intestinal diseases at home and abroad has also attracted widespread attention. Currently, fecal microbiota transplantation is performed clinically by taking fresh feces from donors to prepare a standard fecal microbiota solution for treatment. This fresh f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12Q1/06
Inventor 张昊种法政李彩凤张钧寿
Owner 南京益恒寿生命科技有限公司
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