Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Hiro

35results about How to "Reduce the effect of activity" patented technology

Label-free cell detection device and method based on light sheet illumination

The invention discloses a label-free cell detection device and method based on light sheet illumination. The method includes the steps that a light sheet generation unit is used for shaping a laser beam into a uniform illumination light sheet with the micron-order thickness through a cylinder lens; the light sheet excites particles or a cell suspension placed in a sample micro-cavity chamber controlled by a precise displacement table to move; a form microscopic image and a two-dimensional light scattering pattern of a single particle or cell are recorded by a detector under the focusing mode and the defocusing mode through an objective lens; and a result is input into an imaging analysis system for image processing and recognizing and classifying. By means of the light sheet illumination method, a stimulation area can be effectively limited, background interference in light scattering imaging is inhibited, and effective stimulation of the single particle or cell and particle size discrimination of the sub-micron resolution ratio level are realized; and a light sheet illumination stimulation two-dimensional light scattering technology can avoid complex dyeing operation and a fluorescence signal detection process, and label-free detection and classification are carried out on aging cells. The label-free cell detection device and method based on light sheet illumination are high in applicability and capable of being popularized.
Owner:SHANDONG UNIV

Preparation process of standard coprophilous fungi freeze-dried powder in transplantation therapy of coprophilous fungi

The invention discloses a preparation process of standard coprophilous fungi freeze-dried powder in transplantation therapy of coprophilous fungi. The preparation process comprises the following steps: (1) preparing standard coprophilous fungi liquid; (2) preparing a freeze-dried protecting agent; and (3) carrying out three-step-method freezing and vacuum drying, wherein the freeze-dried protecting agent is prepared by the steps of respectively adding 10%-20% of mycose, 5%-10% of glycerin and 0.02%-0.05% of vitamin C into sterile normal saline, uniformly mixing, filtering, and removing bacteria. According to the special freeze-dried protecting agent, the activity loss of the coprophilous fungi can be prevented, and the survival rate of the coprophilous fungi is increased; and by virtue ofa special freeze-drying procedure, the coprophilous fungi can rapidly enters a dormant state, so that the activity loss is avoided, and the stability of effective components is effectively protected.Experiments show that by utilizing the freeze-dried powder prepared by virtue of the preparation process, the activity of the coprophilous fungi in excrements is effectively maintained, and the survival rate of the coprophilous fungi is greatly increased.
Owner:南京益恒寿生命科技有限公司

Loaded non-metallocene catalyst, its preparation method and application

The invention relates to a loaded non-metallocene catalyst and its preparation method. The loaded non-metallocene catalyst has the characteristics of simple and feasible preparation process, controllable non-metallocene component content, and substantial copolymerization effect, etc. The invention also relates to application of the loaded non-metallocene catalyst in olefin homopolymerization / copolymerization. Compared with the prior art, during application, the loaded non-metallocene catalyst has high olefin polymerization catalytic activity, small particle size and uniform distribution, and the obtained polymer has a high bulk density.
Owner:CHINA PETROLEUM & CHEM CORP +1

Isoluminol derivative and preparation method and application thereof

The invention discloses an enhanced isoluminol derivative with high luminous intensity and a preparation method thereof, and aims to enhance the luminous intensity of an existing luminol-peroxide system, increase the test sensitivity and detection limit, lower the quantity of antibodies or antigens labelled by a luminescent label, and lower the influence of excessive labelling on the antibody or antigen activity. The invention further discloses a chemiluminescent base solution containing the isoluminol derivative, or a chemiluminescent kit containing the antigens or the antibodies labeled by the isoluminol derivative, and a chemiluminescent immunoassay system, and aims to solve the technical problems of low sensitivity and low detection limit when the existing luminol-peroxide system is used for testing. The invention further provides application of the isoluminol derivate to chemiluminescent immune quantitative analyses.
Owner:SHENZHEN NEW INDS BIOMEDICAL ENG

Loaded non-metallocene catalyst, its preparation method and application

The invention relates to a loaded non-metallocene catalyst and its preparation method. The loaded non-metallocene catalyst has the characteristics of simple and feasible preparation process, controllable non-metallocene component content, and substantial copolymerization effect, etc. The invention also relates to application of the loaded non-metallocene catalyst in olefin homopolymerization / copolymerization. Compared with the prior art, during application, the loaded non-metallocene catalyst has high olefin polymerization catalytic activity, small particle size and uniform distribution, and the obtained polymer has a high bulk density.
Owner:CHINA PETROLEUM & CHEM CORP +1

Preparation technology for standard excrement bacteria solution in excrement bacteria transplantation therapy

The invention discloses a preparation technology for a standard excrement bacteria solution in excrement bacteria transplantation therapy. The preparation technology comprises the following steps: (1) homogenizing excrement: collecting excrement and preparing an excrement diluent; (2) gradually filtering: filtering the excrement diluent with an 80-mesh standard sieve, a 300-mesh standard sieve, a 20mum filter membrane, a 5mu m filter membrane and a 0.8mu m filter membrane in turn and then collecting a filtrate; and (3) centrifuging, thereby acquiring a suspension. According to the invention, the gradual filtering manner is adopted and the filtering tool is arranged, so that the food residue is effectively filtered, the microbial activity in the excrement bacteria is kept and the viable count is increased; the specific buffer solution is capable of more effectively maintaining the bacterial activity in the filtering process; an experiment proves that the preparation technology is capable of removing the harmful food residue and greatly increasing the extraction ratio and survival rate of the excrement bacteria and the operation is simple and effective.
Owner:南京益恒寿生命科技有限公司

Loaded non-metallocene catalyst, its preparation method and application

The invention relates to a loaded non-metallocene catalyst and its preparation method. The loaded non-metallocene catalyst has the characteristics of simple and feasible preparation process, controllable non-metallocene component content, and substantial copolymerization effect, etc. The invention also relates to application of the loaded non-metallocene catalyst in olefin homopolymerization / copolymerization. Compared with the prior art, during application, the loaded non-metallocene catalyst has high olefin polymerization catalytic activity, small particle size and uniform distribution, and the obtained polymer has a high bulk density.
Owner:CHINA PETROLEUM & CHEM CORP +1

Loaded non-metallocene catalyst, its preparation method and application

The invention relates to a loaded non-metallocene catalyst and its preparation method. The loaded non-metallocene catalyst has the characteristics of simple and feasible preparation process, controllable non-metallocene component content, and substantial copolymerization effect, etc. The invention also relates to application of the loaded non-metallocene catalyst in olefin homopolymerization / copolymerization. Compared with the prior art, during application, the loaded non-metallocene catalyst has high olefin polymerization catalytic activity, small particle size and uniform distribution, and the obtained polymer has a high bulk density.
Owner:CHINA PETROLEUM & CHEM CORP +1

Loaded non-metallocene catalyst, its preparation method and application

The invention relates to a loaded non-metallocene catalyst and its preparation method. The loaded non-metallocene catalyst has the characteristics of simple and feasible preparation process, controllable non-metallocene component content, and substantial copolymerization effect, etc. The invention also relates to application of the loaded non-metallocene catalyst in olefin homopolymerization / copolymerization. Compared with the prior art, during application, the loaded non-metallocene catalyst has high olefin polymerization catalytic activity, small particle size and uniform distribution, and the obtained polymer has a high bulk density.
Owner:CHINA PETROLEUM & CHEM CORP +1

Bacillus subtilis controlled release agent and preparing method thereof

The invention discloses a bacillus subtilis controlled release agent. The bacillus subtilis controlled release agent is prepared from, by mass, 1-5 parts of a carrier, 5-10 parts of a capsule wall material and 10-20 parts of bacillus subtilis fermentation broth. A preparing method of the bacillus subtilis controlled release agent includes the steps of firstly, culturing bacillus subtilis; secondly, preparing the capsule wall material; thirdly, adsorbing the bacillus subtilis or the bacillus subtilis fermentation broth through the carrier; fourthly, conducting spray-drying. By means of the technical scheme, the bacillus subtilis controlled release agent has the advantages that the bacillus subtilis and fermentation products thereof are microencapsulated to keep good vigor and stability, living microorganisms or microorganism metabolites are fixed to the carrier to keep the activity in use within the limited regional space through a physical method, and the product has the good microcapsule structure, has good mechanical strength and flexibility, and has the good controlled release effect on a capsule-core microbial agent.
Owner:HENAN INST OF SCI & TECH

Loaded non-metallocene catalyst, its preparation method and application

The invention relates to a loaded non-metallocene catalyst and its preparation method. The loaded non-metallocene catalyst has the characteristics of simple and feasible preparation process, controllable non-metallocene component content, and substantial copolymerization effect, etc. The invention also relates to application of the loaded non-metallocene catalyst in olefin homopolymerization / copolymerization. Compared with the prior art, during application, the loaded non-metallocene catalyst has high olefin polymerization catalytic activity, small size and uniform distribution, and the obtained polymer has a high bulk density.
Owner:CHINA PETROLEUM & CHEM CORP +1

Loaded non-metallocene catalyst, its preparation method and application

The invention relates to a loaded non-metallocene catalyst and its preparation method. The loaded non-metallocene catalyst has the characteristics of simple and feasible process, controllable non-metallocene component content, and substantial copolymerization effect, etc. The invention also relates to application of the loaded non-metallocene catalyst in olefin homopolymerization / copolymerization. Compared with the prior art, during application, the loaded non-metallocene catalyst has high olefin polymerization catalytic activity, small particle size and uniform distribution, and the obtained polymer has a high bulk density.
Owner:CHINA PETROLEUM & CHEM CORP +1

Green and fresh plant forage silage device and method for monogastric animals

The invention relates to the technical field of agricultural silage processing equipment, in particular to a green and fresh plant forage silage device and method for monogastric animals. The silage device comprises a water distributor, a leavening agent tank, a main material conveyer, a raw material conveyor, a pulverizer and a pulverizer conveyor. The raw material conveyor, the pulverizer and the pulverizer conveyor are arranged sequentially. A main material hopper is fixedly installed at the feeding end of the main material conveyor, and a first online moisture detector is fixedly installedin the main material hopper. The water distributor communicates with the interior of a feeding port of the pulverizer through a water conveying pipe. The leavening agent tank communicates with the interior of the pulverizer conveyor through a bacterial liquid pipe. According to the device, online detection of material moisture is achieved, and the moisture content of forage is controlled within astandard range through a moisture adjusting device, so that the forage is fermented fully, and the quality of the forage is guaranteed.
Owner:泰安市亿利德农牧机械有限公司

A continuous production device and production method for synthesizing 2,2'-bipyridine

The invention relates to a continuous production device for synthesizing 2,2'-dipyridyl and a production method. The production device comprises a reaction system, wherein the reaction system comprises a fixed bed reactor for synthesizing 2,2'-dipyridyl from pyridine through direct coupling; a pyridine conveying system and a gas carrying system are connected with the inlet of the fixed bed reactor; a product condensation separation system and a circulation system which is for feeding back unreacted pyridine into the pyridine conveying system are sequentially connected with the outlet of the fixed bed reactor; the product condensation separation system comprises a condenser A and a gas-liquid separator which are arranged in sequence; a membrane separator for separating product hydrogen is mounted at a gas phase outlet of the gas-liquid separator; a recycling system for recycling the product, namely the 2,2'-dipyridyl, is connected with a liquid-phase outlet of the gas-liquid separator;the outlet of the recycling system is connected with the circulation system. By adopting the continuous production device, the conversion per pass of the 2,2'-dipyridyl is greatly increased, high-efficiency production process can be achieved, and a technique is provided for continuous industrial direct-coupling production of 2,2'-dipyridyl.
Owner:HEFEI UNIV OF TECH

A kind of sea urchin-shaped hollow gold-silver alloy nanoparticles and preparation method and application thereof

The invention discloses a sea urchin-shaped hollow gold and silver alloy nano particle and a preparation method and application thereof. The method comprises the following steps: dissolving silver nitrate into water; heating the mixture of the silver nitrate and the water till boiling; adding trisodium citrate aqueous solution in the mixture and keeping boiling for 3-30 minutes to obtain a silver colloid; mixing chloroauric acid aqueous solution and the water; adding the silver colloid under the condition of agitation at the temperature of 15 DEG C; adding hemoporphyrin metalporphyrin aqueous solution and obtaining a reaction solution after 5 min reaction; performing centrifugalization of the reaction solution, washing deposit obtained via centrifugalization, and obtaining the sea urchin-shaped hollow gold and silver alloy nano particle. The diameter of the nano particle is 80 nm-300 nm, the hollow size is 20 nm-50 nm, the length of a stab is 10 nm-30 nm, the diameter of the stab is 10 nm. The preparation method is simple, low in cost, gentle in condition, short in time, easy in process control, and large in production capacity. The obtained nano particle can be well applied to organic molecule detection, earlier detection of tumour and photothermal treatment.
Owner:XI AN JIAOTONG UNIV

A label-free cell detection device and method based on light sheet illumination

The invention discloses a label-free cell detection device and method based on light sheet illumination. The method includes the steps that a light sheet generation unit is used for shaping a laser beam into a uniform illumination light sheet with the micron-order thickness through a cylinder lens; the light sheet excites particles or a cell suspension placed in a sample micro-cavity chamber controlled by a precise displacement table to move; a form microscopic image and a two-dimensional light scattering pattern of a single particle or cell are recorded by a detector under the focusing mode and the defocusing mode through an objective lens; and a result is input into an imaging analysis system for image processing and recognizing and classifying. By means of the light sheet illumination method, a stimulation area can be effectively limited, background interference in light scattering imaging is inhibited, and effective stimulation of the single particle or cell and particle size discrimination of the sub-micron resolution ratio level are realized; and a light sheet illumination stimulation two-dimensional light scattering technology can avoid complex dyeing operation and a fluorescence signal detection process, and label-free detection and classification are carried out on aging cells. The label-free cell detection device and method based on light sheet illumination are high in applicability and capable of being popularized.
Owner:SHANDONG UNIV

Droplet jetting device and related method

The invention provides a droplet jetting device and a related method. The droplet jetting device comprises a piezoelectric chip and an interdigital transducer arranged on the upper surface of the piezoelectric chip, the ratio of the thickness of the piezoelectric chip to the period of the interdigital transducer is 1-3, the interdigital transducer is driven by a radio frequency signal to excite surface acoustic waves and bulk acoustic waves in the piezoelectric chip, and the surface acoustic waves and the bulk acoustic waves are reflected on the upper surface and the lower surface of the piezoelectric chip to generate composite acoustic waves so as to drive to-be-driven liquid on a liquid attachment area to stretch in the direction perpendicular to the surface of the piezoelectric chip to form a liquid column, so that the liquid column generates a liquid drop pinch-off effect at the end part, and a single liquid drop is ejected, the liquid attachment area is located on the upper surface and / or the lower surface of the piezoelectric chip and corresponds to the area covered by the surface acoustic wave generated by the interdigital transducer propagating along the surface of the piezoelectric chip. The problems that a device is complex in structure, high in cost, low in efficiency and inconvenient to achieve on-demand jetting are solved.
Owner:HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL

Rural sludge organic fertilizer and preparation method thereof

The invention discloses a rural sludge organic fertilizer and a preparation method thereof. The organic fertilizer comprises the following raw materials in parts by mass: 20-35 parts of dried and crushed rural sludge, 15-25 parts of a beneficial bacterium culture solution, 5-6 parts of inorganic minerals, 0.6-1 part of a binder, 0.3-0.5 part of an anti-caking agent and 6-8 parts of molasses. The beneficial bacterium culture solution is a mixed culture solution of actinomycetes, lactic acid bacteria, saccharomycetes and bacillus. According to the rural sludge organic fertilizer, harmful substances such as pathogenic bacteria and parasites in sludge are killed through sludge high-temperature drying pretreatment; fermentation treatment is performed on the rural sludge by utilizing the aerobic bacteria property of the beneficial bacteria culture solution; the formed organic fertilizer avoids high-temperature sterilization, maintains the beneficial bacterium property of a beneficial bacterium culture solution, and improves the microbial activity of soil and the fertility of the soil by combining organic matters, nitrogen, phosphorus, potassium and other elements in the sludge.
Owner:HOHAI UNIV

A storage reagent for biotinylated inorganic pyrophosphatase

The invention provides a storage reagent for biotinylation inorganic pyrophosphatase. The storage reagent is prepared from 60-90 mM of Tris-HCL with the pH being 8.5, 25-75 mM of calcium chloride, 25-75 mM of magnesium chloride, 20-35% ethanediol and 2-4 mM of DTT. The solvent is deionized water. The storage reagent can protect the activity of the biotinylation inorganic pyrophosphatase, the storage time of the biotinylation inorganic pyrophosphatase can be prolonged, the influence caused by repeated freezing thawing on the activity of the biotinylation inorganic pyrophosphatase is lowered, and the phenomenon that biotin labels on the biotinylation inorganic pyrophosphatase fall down can be effectively reduced.
Owner:BEIJING ZHONGKEZIXIN TECH

A droplet ejection device and related method

The present disclosure provides a droplet ejection device and related methods. The droplet ejection device comprises: a piezoelectric chip, an interdigital transducer arranged on the upper surface of the piezoelectric chip; the ratio of the thickness of the piezoelectric chip to the period of the interdigital transducer is 1 to 3 ; The interdigital transducer excites surface acoustic waves and bulk acoustic waves in the piezoelectric chip under the drive of radio frequency signals, and the surface acoustic waves and the bulk acoustic waves are reflected on the upper and lower surfaces of the piezoelectric chip to generate recombination Acoustic waves, to drive the liquid to be driven on the liquid attachment area to stretch along the direction perpendicular to the surface of the piezoelectric chip to form a liquid column, so that the liquid column will have a droplet pinch-off effect at the end, and a single droplet will be ejected; the liquid The attachment area is located on the upper surface and / or the lower surface of the piezoelectric chip corresponding to the area covered by the propagation of the surface acoustic wave generated by the interdigital transducer along the surface of the piezoelectric chip. It solves the problems of complicated device structure, high cost, low efficiency, and inconvenient realization of on-demand injection.
Owner:HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL

Supported non-metallocene catalyst, its preparation method and application

The invention relates to a loaded non-metallocene catalyst and its preparation method. The loaded non-metallocene catalyst has the characteristics of simple and feasible preparation process, controllable non-metallocene component content, and substantial copolymerization effect, etc. The invention also relates to application of the loaded non-metallocene catalyst in olefin homopolymerization / copolymerization. Compared with the prior art, during application, the loaded non-metallocene catalyst has high olefin polymerization catalytic activity, small particle size and uniform distribution, and the obtained polymer has a high bulk density.
Owner:CHINA PETROLEUM & CHEM CORP +1

Making method of ginger wafer biscuit capable of regulating intestinal flora

The invention belongs to the technical field of food development, and particularly relates to a making method of ginger wafer biscuits capable of regulating intestinal flora. A microwave-assisted three-phase distribution (TPP) combined method is adopted and applied to extraction of ginger protease; freeze spray drying is carried out after microencapsulation treatment, and since a grease body has stable chemical properties and physical properties, the influence on the activity of the ginger protease in subsequent drying can be reduced when the grease body is used for microencapsulation embedding of the ginger protease; glycyrrhizin and ginger oleoresin synergistically enhance the antibacterial function; and lactobacillus plantarum LIP-1 and the ginger protease have a synergistic effect, so that the effect of regulating the intestinal flora is realized.
Owner:UNIV OF JINAN

Supported non-metallocene catalyst, its preparation method and application

The invention relates to a loaded non-metallocene catalyst and its preparation method. The loaded non-metallocene catalyst has the characteristics of simple and feasible preparation process, controllable non-metallocene component content, and substantial copolymerization effect, etc. The invention also relates to application of the loaded non-metallocene catalyst in olefin homopolymerization / copolymerization. Compared with the prior art, during application, the loaded non-metallocene catalyst has high olefin polymerization catalytic activity, small particle size and uniform distribution, and the obtained polymer has a high bulk density.
Owner:CHINA PETROLEUM & CHEM CORP +1

A kind of silage equipment and method for monogastric animal fresh plant feed silage

The present invention relates to the technical field of agricultural processing silage equipment, in particular to a kind of silage equipment and method for monogastric animal green and fresh plant feed. The raw material conveying auger, the pulverizer, and the pulverizer conveying auger are arranged in sequence. The main material hopper is fixedly installed at the feeding end of the main material conveying auger, and the first moisture online detector is fixedly installed in the main material hopper. The container communicates with the inside of the pulverizer feeding port through the water delivery pipe, and the starter tank communicates with the inside of the pulverizer feeding auger through the bacteria liquid pipe. This device realizes the on-line detection of material moisture, and ensures that the moisture content of the feed is controlled within the standard range through the moisture adjustment device, so that the feed fermentation can fully guarantee the quality of the feed.
Owner:泰安市亿利德农牧机械有限公司

Supported non-metallocene catalyst, its preparation method and application

The invention relates to a loaded non-metallocene catalyst and its preparation method. The loaded non-metallocene catalyst has the characteristics of simple and feasible preparation process, controllable non-metallocene component content, and substantial copolymerization effect, etc. The invention also relates to application of the loaded non-metallocene catalyst in olefin homopolymerization / copolymerization. Compared with the prior art, during application, the loaded non-metallocene catalyst has high olefin polymerization catalytic activity, small particle size and uniform distribution, and the obtained polymer has a high bulk density.
Owner:CHINA PETROLEUM & CHEM CORP +1

Supported non-metallocene catalyst, its preparation method and application

The invention relates to a loaded non-metallocene catalyst and its preparation method. The loaded non-metallocene catalyst has the characteristics of simple and feasible preparation process, controllable non-metallocene component content, and substantial copolymerization effect, etc. The invention also relates to application of the loaded non-metallocene catalyst in olefin homopolymerization / copolymerization. Compared with the prior art, during application, the loaded non-metallocene catalyst has high olefin polymerization catalytic activity, small particle size and uniform distribution, and the obtained polymer has a high bulk density.
Owner:CHINA PETROLEUM & CHEM CORP +1

Storage reagent for biotinylated ATPS (adenosine triphosphate sulfurylase)

The invention provides a storage reagent for biotinylated ATPS (adenosine triphosphate sulfurylase). The storage reagent comprises components as follows: Tris-HCl, sodium chloride, EDTA (ethylene diamine tetraacetic acid) and DTT (dithiothreitol), and a solvent adopts deionized water. The storage reagent can prolong the storage time of the biotinylated ATPS and reduce the influence of multigelation on the activity of the biotinylated ATPS; besides, the biotinylated ATPS can be stored at the temperatures of subzero 80 DEG C, subzero 20 DEG C and 4 DEG C for a long time, and the selectivity of storage temperatures of the biotinylated ATPS is increased; the falling-off rate of a biotin marker on the ATPS can also be effectively decreased.
Owner:BEIJING ZHONGKEZIXIN TECH

Rapid parking method of methanol to olefins reaction-regeneration system

The invention relates to a rapid stop method in a methanol-to-olefin reaction-regeneration system, mainly solves the problems of long stop time and non-uniform catalyst coke burning and activity loss during the stop process in the prior art. The method comprises the following steps: a. gradually cutting the materials containing methanol out of a reactor and cutting the vapor in the reacting system; b. continuously cycling the catalyst in a reaction-regeneration system; c. spraying burning oil at the dense phase section of the regenerator when the temperature of the regenerator is lower than 600 DEG C; d. stopping spraying the burning oil to the regenerator after the coke content of the catalyst is less than 0.5% by weight; e. closing a slide valve on a regenerator inclined tube when the temperature is down to 500-550 DEG C; f. conveying the catalyst in the reactor to the regenerator while unloading the catalyst in the regenerator; g. reducing the pressure of the reaction-regenerationsystem after finishing unloading. The technique scheme of the invention well solves the above problem and is suitable to the industrial production of light olefins.
Owner:CHINA PETROLEUM & CHEM CORP +1
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products