35results about How to "Reduce the effect of activity" patented technology

The invention discloses a label-free cell detection device and method based on light sheet illumination. The method includes the steps that a light sheet generation unit is used for shaping a laser beam into a uniform illumination light sheet with the micron-order thickness through a cylinder lens; the light sheet excites particles or a cell suspension placed in a sample micro-cavity chamber controlled by a precise displacement table to move; a form microscopic image and a two-dimensional light scattering pattern of a single particle or cell are recorded by a detector under the focusing mode and the defocusing mode through an objective lens; and a result is input into an imaging analysis system for image processing and recognizing and classifying. By means of the light sheet illumination method, a stimulation area can be effectively limited, background interference in light scattering imaging is inhibited, and effective stimulation of the single particle or cell and particle size discrimination of the sub-micron resolution ratio level are realized; and a light sheet illumination stimulation two-dimensional light scattering technology can avoid complex dyeing operation and a fluorescence signal detection process, and label-free detection and classification are carried out on aging cells. The label-free cell detection device and method based on light sheet illumination are high in applicability and capable of being popularized.

The invention discloses a preparation process of standard coprophilous fungi freeze-dried powder in transplantation therapy of coprophilous fungi. The preparation process comprises the following steps: (1) preparing standard coprophilous fungi liquid; (2) preparing a freeze-dried protecting agent; and (3) carrying out three-step-method freezing and vacuum drying, wherein the freeze-dried protecting agent is prepared by the steps of respectively adding 10%-20% of mycose, 5%-10% of glycerin and 0.02%-0.05% of vitamin C into sterile normal saline, uniformly mixing, filtering, and removing bacteria. According to the special freeze-dried protecting agent, the activity loss of the coprophilous fungi can be prevented, and the survival rate of the coprophilous fungi is increased; and by virtue ofa special freeze-drying procedure, the coprophilous fungi can rapidly enters a dormant state, so that the activity loss is avoided, and the stability of effective components is effectively protected.Experiments show that by utilizing the freeze-dried powder prepared by virtue of the preparation process, the activity of the coprophilous fungi in excrements is effectively maintained, and the survival rate of the coprophilous fungi is greatly increased.

The invention relates to a loaded non-metallocene catalyst and its preparation method. The loaded non-metallocene catalyst has the characteristics of simple and feasible preparation process, controllable non-metallocene component content, and substantial copolymerization effect, etc. The invention also relates to application of the loaded non-metallocene catalyst in olefin homopolymerization/copolymerization. Compared with the prior art, during application, the loaded non-metallocene catalyst has high olefin polymerization catalytic activity, small particle size and uniform distribution, and the obtained polymer has a high bulk density.

The invention discloses an enhanced isoluminol derivative with high luminous intensity and a preparation method thereof, and aims to enhance the luminous intensity of an existing luminol-peroxide system, increase the test sensitivity and detection limit, lower the quantity of antibodies or antigens labelled by a luminescent label, and lower the influence of excessive labelling on the antibody or antigen activity. The invention further discloses a chemiluminescent base solution containing the isoluminol derivative, or a chemiluminescent kit containing the antigens or the antibodies labeled by the isoluminol derivative, and a chemiluminescent immunoassay system, and aims to solve the technical problems of low sensitivity and low detection limit when the existing luminol-peroxide system is used for testing. The invention further provides application of the isoluminol derivate to chemiluminescent immune quantitative analyses.

The invention relates to a loaded non-metallocene catalyst and its preparation method. The loaded non-metallocene catalyst has the characteristics of simple and feasible preparation process, controllable non-metallocene component content, and substantial copolymerization effect, etc. The invention also relates to application of the loaded non-metallocene catalyst in olefin homopolymerization/copolymerization. Compared with the prior art, during application, the loaded non-metallocene catalyst has high olefin polymerization catalytic activity, small particle size and uniform distribution, and the obtained polymer has a high bulk density.

The invention discloses a preparation technology for a standard excrement bacteria solution in excrement bacteria transplantation therapy. The preparation technology comprises the following steps: (1) homogenizing excrement: collecting excrement and preparing an excrement diluent; (2) gradually filtering: filtering the excrement diluent with an 80-mesh standard sieve, a 300-mesh standard sieve, a 20mum filter membrane, a 5mu m filter membrane and a 0.8mu m filter membrane in turn and then collecting a filtrate; and (3) centrifuging, thereby acquiring a suspension. According to the invention, the gradual filtering manner is adopted and the filtering tool is arranged, so that the food residue is effectively filtered, the microbial activity in the excrement bacteria is kept and the viable count is increased; the specific buffer solution is capable of more effectively maintaining the bacterial activity in the filtering process; an experiment proves that the preparation technology is capable of removing the harmful food residue and greatly increasing the extraction ratio and survival rate of the excrement bacteria and the operation is simple and effective.

The invention relates to a loaded non-metallocene catalyst and its preparation method. The loaded non-metallocene catalyst has the characteristics of simple and feasible preparation process, controllable non-metallocene component content, and substantial copolymerization effect, etc. The invention also relates to application of the loaded non-metallocene catalyst in olefin homopolymerization/copolymerization. Compared with the prior art, during application, the loaded non-metallocene catalyst has high olefin polymerization catalytic activity, small particle size and uniform distribution, and the obtained polymer has a high bulk density.

The invention relates to a loaded non-metallocene catalyst and its preparation method. The loaded non-metallocene catalyst has the characteristics of simple and feasible preparation process, controllable non-metallocene component content, and substantial copolymerization effect, etc. The invention also relates to application of the loaded non-metallocene catalyst in olefin homopolymerization/copolymerization. Compared with the prior art, during application, the loaded non-metallocene catalyst has high olefin polymerization catalytic activity, small particle size and uniform distribution, and the obtained polymer has a high bulk density.

The invention relates to a loaded non-metallocene catalyst and its preparation method. The loaded non-metallocene catalyst has the characteristics of simple and feasible preparation process, controllable non-metallocene component content, and substantial copolymerization effect, etc. The invention also relates to application of the loaded non-metallocene catalyst in olefin homopolymerization/copolymerization. Compared with the prior art, during application, the loaded non-metallocene catalyst has high olefin polymerization catalytic activity, small particle size and uniform distribution, and the obtained polymer has a high bulk density.

The invention relates to a loaded non-metallocene catalyst and its preparation method. The loaded non-metallocene catalyst has the characteristics of simple and feasible preparation process, controllable non-metallocene component content, and substantial copolymerization effect, etc. The invention also relates to application of the loaded non-metallocene catalyst in olefin homopolymerization/copolymerization. Compared with the prior art, during application, the loaded non-metallocene catalyst has high olefin polymerization catalytic activity, small size and uniform distribution, and the obtained polymer has a high bulk density.

The invention relates to a loaded non-metallocene catalyst and its preparation method. The loaded non-metallocene catalyst has the characteristics of simple and feasible process, controllable non-metallocene component content, and substantial copolymerization effect, etc. The invention also relates to application of the loaded non-metallocene catalyst in olefin homopolymerization/copolymerization. Compared with the prior art, during application, the loaded non-metallocene catalyst has high olefin polymerization catalytic activity, small particle size and uniform distribution, and the obtained polymer has a high bulk density.

The invention relates to the technical field of agricultural silage processing equipment, in particular to a green and fresh plant forage silage device and method for monogastric animals. The silage device comprises a water distributor, a leavening agent tank, a main material conveyer, a raw material conveyor, a pulverizer and a pulverizer conveyor. The raw material conveyor, the pulverizer and the pulverizer conveyor are arranged sequentially. A main material hopper is fixedly installed at the feeding end of the main material conveyor, and a first online moisture detector is fixedly installedin the main material hopper. The water distributor communicates with the interior of a feeding port of the pulverizer through a water conveying pipe. The leavening agent tank communicates with the interior of the pulverizer conveyor through a bacterial liquid pipe. According to the device, online detection of material moisture is achieved, and the moisture content of forage is controlled within astandard range through a moisture adjusting device, so that the forage is fermented fully, and the quality of the forage is guaranteed.

The invention discloses a rural sludge organic fertilizer and a preparation method thereof. The organic fertilizer comprises the following raw materials in parts by mass: 20-35 parts of dried and crushed rural sludge, 15-25 parts of a beneficial bacterium culture solution, 5-6 parts of inorganic minerals, 0.6-1 part of a binder, 0.3-0.5 part of an anti-caking agent and 6-8 parts of molasses. The beneficial bacterium culture solution is a mixed culture solution of actinomycetes, lactic acid bacteria, saccharomycetes and bacillus. According to the rural sludge organic fertilizer, harmful substances such as pathogenic bacteria and parasites in sludge are killed through sludge high-temperature drying pretreatment; fermentation treatment is performed on the rural sludge by utilizing the aerobic bacteria property of the beneficial bacteria culture solution; the formed organic fertilizer avoids high-temperature sterilization, maintains the beneficial bacterium property of a beneficial bacterium culture solution, and improves the microbial activity of soil and the fertility of the soil by combining organic matters, nitrogen, phosphorus, potassium and other elements in the sludge.