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Bacillus subtilis controlled release agent and preparing method thereof

A technology of Bacillus subtilis and controlled release agent, which is applied in the directions of botanical equipment and methods, biochemical equipment and methods, fungicides, etc. Wettable powder dust flying and other problems, to achieve good microcapsule structure, good controlled release effect, reduce the effect of the impact

Inactive Publication Date: 2019-10-11
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, wettable powders have problems such as flying dust, polluting the environment, and not being able to maintain efficacy after long-term storage.
Water-dispersible granules are a relatively new type of biological pesticide formulation, which can solve the problems of wettable powders such as environmental pollution and flying dust, but still cannot effectively solve the problem of unstable storage of microbial live pesticide preparations

Method used

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  • Bacillus subtilis controlled release agent and preparing method thereof
  • Bacillus subtilis controlled release agent and preparing method thereof
  • Bacillus subtilis controlled release agent and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Strain activation:

[0028] Take out the Bacillus subtilis HU2012 stored in the refrigerator at 4°C, and place it at room temperature for 10 minutes. Then use an inoculation loop to inoculate the pre-made MHA medium by streaking on a plate, and carry out activation culture at 37±1°C for 24 hours, and set aside.

[0029] (2) Preparation of seed medium:

[0030] The components of the seed medium are: 200g of potato decoction, 20g of glucose, 1000ml of water is added, and the pH value is adjusted to 7.0.

[0031] (3) Preparation of seed solution: Inoculate activated Bacillus subtilis HU2012 into the above seed medium, ferment at 40° C., and ferment for 12 hours.

[0032] (4) Preparation of fermented liquid: inoculate the above seed liquid into the fermentation medium, the inoculum amount is 1% of the volume of the fermentation medium, the pressure of the fermenter is 0.25MPa, the fermentation temperature is 25°C, and the fermentation time is 48h.

[0033] (5) 500kg ...

Embodiment 2

[0037] (1) Strain activation:

[0038] Take out the Bacillus subtilis HU2012 stored in the refrigerator at 4°C, and place it at room temperature for 10 minutes. Then use an inoculation loop to inoculate the pre-made MHA medium by streaking on a plate, and carry out activation culture at 37±1°C for 24 hours, and set aside.

[0039] (2) Preparation of seed medium:

[0040] The components of the seed medium are: 300g of potato decoction, 10g of glucose, 1000ml of water is added, and the pH value is adjusted to 7.0.

[0041] (3) Preparation of seed solution: Inoculate activated Bacillus subtilis HU2012 into the above seed medium, ferment at 35° C., and ferment for 36 hours.

[0042] (4) Preparation of fermentation liquid: inoculate the above seed liquid into the fermentation medium, the inoculum amount is 5% of the volume of the fermentation medium, the pressure of the fermenter is 0.05MPa, the fermentation temperature is 35°C, and the fermentation time is 24h.

[0043] (5) 100...

Embodiment 3

[0047] (1) Strain activation:

[0048] Take out the Bacillus subtilis HU2012 stored in the refrigerator at 4°C, and place it at room temperature for 10 minutes. Then use an inoculation loop to inoculate the pre-made MHA medium by streaking on a plate, and carry out activation culture at 37±1°C for 24 hours, and set aside.

[0049] (2) Preparation of seed medium:

[0050] The components of the seed medium are: 250g of potato decoction, 15g of glucose, 1000ml of water is added, and the pH value is adjusted to 7.0.

[0051] (3) Preparation of seed solution: Bacillus subtilis HU2012 was inoculated into the above seed medium, the fermentation temperature was 37° C., and the fermentation time was 24 hours.

[0052] (4) Preparation of fermented liquid: inoculate the above seed liquid into the fermentation medium, the inoculum amount is 3% of the volume of the fermentation medium, the pressure of the fermenter is 0.15MPa, the fermentation temperature is 30°C, and the fermentation ti...

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Abstract

The invention discloses a bacillus subtilis controlled release agent. The bacillus subtilis controlled release agent is prepared from, by mass, 1-5 parts of a carrier, 5-10 parts of a capsule wall material and 10-20 parts of bacillus subtilis fermentation broth. A preparing method of the bacillus subtilis controlled release agent includes the steps of firstly, culturing bacillus subtilis; secondly, preparing the capsule wall material; thirdly, adsorbing the bacillus subtilis or the bacillus subtilis fermentation broth through the carrier; fourthly, conducting spray-drying. By means of the technical scheme, the bacillus subtilis controlled release agent has the advantages that the bacillus subtilis and fermentation products thereof are microencapsulated to keep good vigor and stability, living microorganisms or microorganism metabolites are fixed to the carrier to keep the activity in use within the limited regional space through a physical method, and the product has the good microcapsule structure, has good mechanical strength and flexibility, and has the good controlled release effect on a capsule-core microbial agent.

Description

technical field [0001] The invention relates to the technical field of microbial preparations, in particular to a controlled release agent for bacillus subtilis and a preparation method thereof. Background technique [0002] The control of plant diseases at home and abroad is mainly based on chemical control, but problems such as environmental pollution, increased resistance of pests and diseases, pesticide residues and soil micro-ecological damage caused by chemical control have become increasingly prominent. Biological control has been paid more and more attention because of its good environmental compatibility, less disease resistance and no harm to humans and animals. Bacillus subtilis mainly exerts its biocontrol function through mechanisms such as inducing resistance, secreting antibacterial or lipopeptide substances, bacteriolysis, nutrition and space site competition. [0003] At present, the dosage forms of live Bacillus subtilis preparations at home and abroad are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/28A01N63/02A01P3/00C12N1/20C12R1/125
CPCA01N25/28C12N1/20A01N63/10
Inventor 朱红霞刘善芹胡林峰肖香珍苏晓唐东洋陈世敏杨贺贺
Owner HENAN INST OF SCI & TECH
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