Storage reagent for biotinylated ATPS (adenosine triphosphate sulfurylase)

A biotinylation and reagent technology, applied in the biological field, can solve the problems of easy inactivation, ineffective protection of biotinylated ATPS enzyme activity, storage reagents without biotinylated ATPS, etc., so as to reduce the impact and prolong the storage time. Effect

Active Publication Date: 2016-03-30
BEIJING ZHONGKEZIXIN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a biologically active protein, ATPS is easily inactivated during storage, transportation and sales. Currently, commonly used enzyme storage reagents cannot effectively protect the enzyme activity of biotinylated ATPS, and there is no specific storage solution for biotinylated ATPS. Reagent

Method used

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  • Storage reagent for biotinylated ATPS (adenosine triphosphate sulfurylase)
  • Storage reagent for biotinylated ATPS (adenosine triphosphate sulfurylase)
  • Storage reagent for biotinylated ATPS (adenosine triphosphate sulfurylase)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 A kind of storage reagent of biotinylated ATPS

[0025] Including the following components: Tris-HCl25mMpH7.0, sodium chloride10mM, EDTA0.02mM and DTT0.02mM, the solvent is deionized water.

Embodiment 2

[0026] Embodiment 2 A kind of storage reagent of biotinylated ATPS

[0027] Including the following components: Tris-HCl30mMpH7.2, sodium chloride15mM, EDTA0.03mM and DTT0.03mM, the solvent is deionized water.

Embodiment 3

[0028] Embodiment 3 A kind of storage reagent of biotinylated ATPS

[0029] Including the following components: Tris-HCl35mMpH7.4, sodium chloride20mM, EDTA0.04mM and DTT0.04mM, the solvent is deionized water.

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Abstract

The invention provides a storage reagent for biotinylated ATPS (adenosine triphosphate sulfurylase). The storage reagent comprises components as follows: Tris-HCl, sodium chloride, EDTA (ethylene diamine tetraacetic acid) and DTT (dithiothreitol), and a solvent adopts deionized water. The storage reagent can prolong the storage time of the biotinylated ATPS and reduce the influence of multigelation on the activity of the biotinylated ATPS; besides, the biotinylated ATPS can be stored at the temperatures of subzero 80 DEG C, subzero 20 DEG C and 4 DEG C for a long time, and the selectivity of storage temperatures of the biotinylated ATPS is increased; the falling-off rate of a biotin marker on the ATPS can also be effectively decreased.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a storage reagent of biotinylated ATPS. Background technique [0002] ATPsulfurylase (ATPsulfurylase, ATPS, EC2.7.7.4) is a reversible catalyzed reaction between adenosine-5'-phosphosulfate (APS) and pyrophosphate (pyrophosphate, PPi) to generate adenosine triphosphate (ATP) and A sulfate enzyme that plays an important role in the metabolism of sulfur in organisms. ATPS widely exists in plants, animals and microorganisms, but its biological role in various organisms is slightly different. In plants and some microorganisms, it is a key enzyme that catalyzes the first step of the sulfate assimilation reaction. Catalyzed by ATPS, sulfate ions react with ATP to produce adenine-5'-phosphosulfate APS and pyrophosphate PPi. In some chemoautotrophic bacteria and chemoinorganotrophic bacteria, ATPS is the enzyme that catalyzes the oxidation of hydrogen sulfide to generate inorganic sulfate...

Claims

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Application Information

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IPC IPC(8): C12N9/96C12N9/12
CPCC12N9/1241C12N9/96C12Y207/07004
Inventor 高静蔡亦梅吴超徐潇张睿王者馥王绪敏殷金龙任鲁风
Owner BEIJING ZHONGKEZIXIN TECH
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