The invention relates to an inoculating needle used for a sterile operation. The inoculating needle used for the sterile operation comprises a handle, a locking end cover, a nickel alloy spring ring, a connecting rod, a sliding block, a pretight spring and a needle body, wherein a round hole and a cross-shaped seam are formed in a fixed part of the handle, one locking end cover is fastened on the fixed part, one nickel alloy spring ring is sheathed on the upper part of the needle body, one connecting rod is connected with the outer side of the nickel alloy spring ring in a welding way, the connecting rod is penetratively arranged in a groove of the handle along the axial direction, a groove in a mounting part is a pretight spring mounting cavity, an annular secondary auxiliary barrel is sheathed outside a corresponding mounting part, a linear sliding chute and a sliding block are arranged in the axial direction of a secondary barrel, the sliding block is connected with a stirring rod fixed on the connecting rod into a whole by virtue of a fastening structure, and the pre-tight spring can cause that the connecting rod tends to move in the direction far away from one end of the needle body. The sliding block is pushed by virtue of a thumb, so that the nickel alloy spring ring quickly stretches out, a fungus block is fallen off, inoculation time is shortened, long-time contact between a culture medium and the outside air is avoided, and pollution probability is reduced.