Preparation technology for standard excrement bacteria solution in excrement bacteria transplantation therapy

Abstract

The invention discloses a preparation technology for a standard excrement bacteria solution in excrement bacteria transplantation therapy. The preparation technology comprises the following steps: (1) homogenizing excrement: collecting excrement and preparing an excrement diluent; (2) gradually filtering: filtering the excrement diluent with an 80-mesh standard sieve, a 300-mesh standard sieve, a 20mum filter membrane, a 5mu m filter membrane and a 0.8mu m filter membrane in turn and then collecting a filtrate; and (3) centrifuging, thereby acquiring a suspension. According to the invention, the gradual filtering manner is adopted and the filtering tool is arranged, so that the food residue is effectively filtered, the microbial activity in the excrement bacteria is kept and the viable count is increased; the specific buffer solution is capable of more effectively maintaining the bacterial activity in the filtering process; an experiment proves that the preparation technology is capable of removing the harmful food residue and greatly increasing the extraction ratio and survival rate of the excrement bacteria and the operation is simple and effective.

Description

technical field [0001] The invention relates to the field of medical technology, more specifically, to a preparation process of standard fecal bacteria liquid in feces transplantation treatment. Background technique [0002] Fecal microbiota transplantation (FMT) began at least in the 4th century AD in the history of world medicine, but it has not achieved unprecedented rapid development until recent years. In 2013, FMT was written into the American Medical Guidelines for the first time for the treatment of recurrent Clostridium difficile infection (CDI). At the same time, its use in the treatment of inflammatory bowel disease, irritable bowel syndrome, metabolic syndrome and other intestinal and intestinal diseases at home and abroad has also attracted widespread attention. The therapeutic effect of fecal bacteria is the surviving microorganisms in it. Therefore, in the process of preparing fecal bacteria liquid, the procedure should be as simple as possible, and obtaining...

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Problems solved by technology

[0003] The purpose of the present invention is to overcome the defects of the current preparation technology of fecal bacteria liquid with many food residues and loss of a large number of bacteria, and to invent a simple and effective fecal bacteria that can not only keep the microbial activity in the feces bacteria from reducing, but also remove harmful food residues. Bacterial solution preparation method

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