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Use of black sesame pigment and its preparation method

A technology of black sesame pigment and black sesame, which is applied in the field of medicine, can solve the problems of unfavorable biological activity research and subsequent utilization, and many impurities of melanin

Active Publication Date: 2021-06-01
瑞康东方医学研究(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method adopts non-toxic process, simple equipment and convenient use, but the obtained melanin has many impurities, which is not conducive to the research of biological activity and subsequent utilization

Method used

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  • Use of black sesame pigment and its preparation method
  • Use of black sesame pigment and its preparation method
  • Use of black sesame pigment and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation of embodiment 1 black sesame pigment

[0036] The preparation method comprises the following steps:

[0037] S1. Select mature black sesame seeds, remove impurities, pulverize them with a grinder for 30 seconds, stop for 1 minute, and repeat 5 times to obtain black sesame powder;

[0038] S2, take the black sesame powder obtained in step S1, add freshly prepared 10g / L sodium hydroxide solution, the mass volume ratio of black sesame powder and sodium hydroxide is 1:10, and place it in a stirring tank at 120rpm to stir at 30°C Dissolve, centrifuge at 8000rpm for 15min after 40min, remove the precipitate, and obtain a black supernatant;

[0039] S3, take the black supernatant obtained in step S2, let it stand for 20min, remove the upper layer of grease through a separatory funnel, add 1.5mol / L hydrochloric acid solution under 120rpm stirring conditions, adjust the pH to 2.0, continue stirring for 15min, and centrifuge at 8000rpm 15min, remove the supernatan...

Embodiment 2

[0041] The preparation of embodiment 2 black sesame pigment

[0042] The difference from Example 1 is that step S1 of Example 2 is to select mature black sesame seeds, remove impurities, grind them with a grinder for 35 seconds, stop for 1 minute, and repeat 5 times to obtain black sesame powder. For the remaining operating steps, refer to Example 1.

Embodiment 3

[0043] The preparation of embodiment 3 black sesame pigment

[0044] The difference from Example 1 is that step S1 of Example 3 is to select mature black sesame seeds, remove impurities, grind them with a grinder for 45 seconds, stop for 1 minute, and repeat 3 times to obtain black sesame powder. For the remaining operating steps, refer to Example 1.

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Abstract

The invention belongs to the technical field of medicine, and in particular relates to the use of black sesame pigment and a preparation method thereof. Tests have confirmed that the black sesame pigment prepared by the present invention has significant cellular antioxidant activity and antitumor activity, and can significantly inhibit the growth of cancer cells (HGC, AGS, SGC-7901, A549, HEPG-2 and MCF-7) , the mortality rate of cancer cells is as high as 95-99%, and it can be used in the preparation of anti-tumor drugs or health products for cancer patients, special medical food.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the use of black sesame pigment and a preparation method thereof. Background technique [0002] Cancer is a disease that seriously threatens human life and health in the world today and has a high incidence rate. It is one of the main causes of human death worldwide. The treatment of malignant tumors has also become a common problem in the field of life sciences. At present, the methods commonly used in clinical treatment of cancer mainly include surgery, chemotherapy, radiotherapy, etc. However, while radiotherapy and chemotherapy kill tumor cells in patients, they also destroy normal cells and immune cells in the body, causing damage to various tissues and organs, causing Serious side effects such as bone marrow suppression, decreased immunity, liver and kidney damage, and cardiotoxicity. In addition, patients will develop drug resistance during the use of chemoth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A23L33/105A61P39/06A61P35/00A61K131/00
CPCA23V2002/00A61K36/185A23L33/105A61P35/00A61P39/06A23V2200/308A23V2250/21
Inventor 周林吴继宏陈娟朱爽张巨峰
Owner 瑞康东方医学研究(北京)有限公司
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