The invention provides a single
cell sorting and printing integrated device and method. The device comprises a shell structure, an X-axis displacement mechanism, a Y-axis displacement mechanism, a printing head, a first visual module, a second visual module, a carrying table and a control module. The X-axis displacement mechanism and the Y-axis displacement mechanism are installed on the shell structure, and the Y axis is located below the X-axis displacement mechanism. The printing head is arranged on the X-axis displacement mechanism, a printing
chip for realizing
liquid injection through thermal bubbles is arranged on the printing head, the printing
chip is provided with a plurality of nozzles which are independently controlled, and a liquid adding port of the printing head is open and forms a straight-through liquid path with the nozzles. The first visual module is installed on the X-axis displacement mechanism with a lens facing downwards, and the second visual module is installed on the Y-axis displacement mechanism with a lens facing upwards. And the carrying table is arranged on the Y-axis displacement mechanism and is provided with the pore plate. The control module is electrically connected with the X-axis displacement mechanism, the Y-axis displacement mechanism, the printing
chip, the first vision module and the second vision module. According to the application, high-efficiency cooperation of single
cell sorting, micro liquid sample application and
cell printing is realized, and a closed-loop process from cell screening, waste liquid treatment to printing is completed.