Rhizobium and application thereof

A rhizobia and microbial strain technology, applied in the field of microorganisms, can solve problems such as easily destroying soil structure and environmental pollution, and achieve the effect of promoting plant growth and increasing yield

Active Publication Date: 2018-12-28
XIAN UNVERSITY OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Potassium and phosphorus in soil mostly exist in minerals such as aluminosilicate and apatite. Potassium and phosphorus in the form of mineral potassium and mineral phosphorus cannot be directly absorb

Method used

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  • Rhizobium and application thereof
  • Rhizobium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0036] Screening of strains:

[0037] Step 1. Enrichment: Collect soil samples of about 20g each from the Ziwei Garden, Baisha Road, Bowen Road Square and Ganjiazhai, Xi'an City, 10cm below the surface, and put the collected soil samples in an oven to dry , pass the dried soil through a 100-mesh sieve, accurately weigh 1 g of the soil that has passed through a 100-mesh sieve in 9 mL of sterile water in a sterile manner, let it stand for 30 minutes, and shake it on a shaker for 30 minutes at room temperature to obtain a dilution of 10 -1 Aspirate 1mL from the above soil suspension, add 9mL sterile water, and mix well to obtain a dilution of 10 -2 of soil suspensions; from a dilution of 10 -2 Aspirate 1 mL of the soil suspension, add it to 9 mL of sterile water, and mix thoroughly to obtain a dilution of 10. -3 the soil suspension;

[0038] Step 2, primary screening and purification: take the dilution in step 1 as 10 -30.1 mL of the soil suspension was coated in the potassiu...

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Abstract

The invention discloses a rhizobium sp. WP 1-3, and the preservation number is CGMCC No.14472. In addition, the invention further discloses the application of the rhizobium to decomposition of potassium in soil and decomposition of insoluble phosphorus in the soil. The rhizobium is a dominant strain screened by taking potash feldsparf as a potassium source, the strain is capable of transforming the insoluble potassium in the aluminosilicate in the soil into a soluble component in the processes of growth and propagation, and promoting the decomposition of the insoluble potassium to increase thesource of the phosphorus in the soil. In addition, the strain further has an effect of fixing nitrogen, and provides the necessary nitrogen for the growth and metabolism of a plant.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a rhizobium and its application. Background technique [0002] Potassium and phosphorus are important nutrients in plants, which are necessary for biological nitrogen fixation and are also the key components to promote plant growth and metabolism. Potassium and phosphorus play an important role in regulating cell penetration, material transport, promoting enzyme activation, promoting photosynthesis, increasing carbon dioxide assimilation rate, promoting the synthesis and transport of substances in crops, and enhancing plant resistance and tolerance. Potassium and phosphorus in soil mostly exist in minerals such as aluminosilicate and apatite. Potassium and phosphorus in the form of mineral potassium and mineral phosphorus cannot be directly absorbed by plants. Chemical potassium and chemical phosphorus are commonly used in agriculture. The way of supplementing...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/14C12R1/41C09K101/00
CPCC09K17/14C09K2101/00C12N1/205C12R2001/41
Inventor 赵咏梅杨银银王华薛杨
Owner XIAN UNVERSITY OF ARTS & SCI
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