A screening and domestication method of microbial flora for garden water purification
A microbial flora and water purification technology, applied in the direction of using microorganisms, biochemical equipment and methods, microorganisms, etc., can solve problems such as difficult operation, long breeding period and insufficient adaptability
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Embodiment 1
[0017] The screening and domestication method of the microbial flora that is used for garden water purification of embodiment 1 may further comprise the steps:
[0018] ①Preparation of enrichment medium: In 1L of sterile water, add 1.4g / L of yeast extract, 5.0g / L of glucose, 3.0g / L of beef extract, and 6.9g / L of peptone in 1L of sterile water. L, urea 1.1g / L, sodium chloride 4.8g / L and potassium dihydrogen phosphate 5.9g / L, prepare the first-generation enrichment medium, and adjust the pH value of the first-generation enrichment medium to 6.4; 1L of sterile water, based on the volume of the 1L of sterile water, add yeast extract 0.9g / L, glucose 3.2g / L, beef extract 1.4g / L, peptone 3.6g / L, urea 0.6g / L, chlorine Sodium chloride 3.0g / L and potassium dihydrogen phosphate 2.9g / L are prepared to obtain the second-generation enrichment medium, and the pH value of the second-generation enrichment medium is adjusted to 5.2;
[0019] ②Collection of bacteria source samples: In June, 100...
Embodiment 2
[0030] The screening and domestication method of the microbial flora that is used for garden water purification of embodiment 2 may further comprise the steps:
[0031] ①Preparation of enrichment medium: In 1L of sterile water, add 1.5g / L of yeast extract, 5.3g / L of glucose, 3.1g / L of beef extract, and 7.2g / L of peptone in 1L of sterile water. L, urea 1.2g / L, sodium chloride 5.0g / L and potassium dihydrogen phosphate 6.0g / L, prepare the first-generation enrichment medium, and adjust the pH value of the first-generation enrichment medium to 6.5; 1L of sterile water, based on the volume of the 1L of sterile water, add yeast extract 1.0g / L, glucose 3.3g / L, beef extract 1.3g / L, peptone 3.8g / L, urea 0.7g / L, chlorine Sodium chloride 3.2g / L and potassium dihydrogen phosphate 3.0g / L are prepared to obtain the second-generation enrichment medium, and the pH value of the second-generation enrichment medium is adjusted to 5.8;
[0032] ②Collection of bacterial source samples: In July, 15...
Embodiment 3
[0040] The screening and domestication method of the microbial flora that is used for garden water purification of embodiment 3 may further comprise the steps:
[0041] ① Preparation of enrichment medium: In 1L of sterile water, add 1.6g / L of yeast extract, 5.4g / L of glucose, 3.2g / L of beef extract, and 7.0g / L of peptone in 1L of sterile water. L, urea 1.1g / L, sodium chloride 5.1g / L and potassium dihydrogen phosphate 6.2g / L, prepare the first-generation enrichment medium, and adjust the pH value of the first-generation enrichment medium to 6.6; 1L of sterile water, based on the volume of the 1L of sterile water, add yeast extract 1.1g / L, glucose 3.4g / L, beef extract 1.4g / L, peptone 4.0g / L, urea 0.8g / L, chlorine Sodium chloride 3.0g / L and potassium dihydrogen phosphate 3.0g / L are prepared to obtain the second-generation enrichment medium, and the pH value of the second-generation enrichment medium is adjusted to 6.4;
[0042] ②Collection of bacterial source samples: In Septemb...
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