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Screening and domesticating method of microbial flora used for purifying water quality of gardens

A technology for microbial flora and water purification, which is applied in the methods of using microorganisms, biochemical equipment and methods, microorganisms, etc., and can solve the problems of complex breeding, long cycle, insufficient adaptability, and difficult operation.

Active Publication Date: 2016-05-04
NINGBO JINGQI PROPERTY DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After research and practice in recent years, although some water purification microorganisms have been found to purify water bodies, the actual application has found that their adaptability is not strong enough, the breeding cycle is long, difficult to operate, and the breeding cost of bacteria is high.

Method used

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  • Screening and domesticating method of microbial flora used for purifying water quality of gardens
  • Screening and domesticating method of microbial flora used for purifying water quality of gardens
  • Screening and domesticating method of microbial flora used for purifying water quality of gardens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The screening and domestication method of the microbial flora that is used for garden water purification of embodiment 1 may further comprise the steps:

[0018] ①Preparation of enrichment medium: In 1L of sterile water, add 1.4g / L of yeast extract, 5.0g / L of glucose, 3.0g / L of beef extract, and 6.9g / L of peptone in 1L of sterile water. L, urea 1.1g / L, sodium chloride 4.8g / L and potassium dihydrogen phosphate 5.9g / L, prepare the first-generation enrichment medium, and adjust the pH value of the first-generation enrichment medium to 6.4; 1L of sterile water, based on the volume of the 1L of sterile water, add yeast extract 0.9g / L, glucose 3.2g / L, beef extract 1.4g / L, peptone 3.6g / L, urea 0.6g / L, chlorine Sodium chloride 3.0g / L and potassium dihydrogen phosphate 2.9g / L are prepared to obtain the second-generation enrichment medium, and the pH value of the second-generation enrichment medium is adjusted to 5.2;

[0019] ②Collection of bacteria source samples: In June, 100...

Embodiment 2

[0030] The screening and domestication method of the microbial flora that is used for garden water purification of embodiment 2 may further comprise the steps:

[0031] ①Preparation of enrichment medium: In 1L of sterile water, add 1.5g / L of yeast extract, 5.3g / L of glucose, 3.1g / L of beef extract, and 7.2g / L of peptone in 1L of sterile water. L, urea 1.2g / L, sodium chloride 5.0g / L and potassium dihydrogen phosphate 6.0g / L, prepare the first-generation enrichment medium, and adjust the pH value of the first-generation enrichment medium to 6.5; 1L of sterile water, based on the volume of the 1L of sterile water, add yeast extract 1.0g / L, glucose 3.3g / L, beef extract 1.3g / L, peptone 3.8g / L, urea 0.7g / L, chlorine Sodium chloride 3.2g / L and potassium dihydrogen phosphate 3.0g / L are prepared to obtain the second-generation enrichment medium, and the pH value of the second-generation enrichment medium is adjusted to 5.8;

[0032] ②Collection of bacterial source samples: In July, 15...

Embodiment 3

[0040] The screening and domestication method of the microbial flora that is used for garden water purification of embodiment 3 may further comprise the steps:

[0041] ① Preparation of enrichment medium: In 1L of sterile water, add 1.6g / L of yeast extract, 5.4g / L of glucose, 3.2g / L of beef extract, and 7.0g / L of peptone in 1L of sterile water. L, urea 1.1g / L, sodium chloride 5.1g / L and potassium dihydrogen phosphate 6.2g / L, prepare the first-generation enrichment medium, and adjust the pH value of the first-generation enrichment medium to 6.6; 1L of sterile water, based on the volume of the 1L of sterile water, add yeast extract 1.1g / L, glucose 3.4g / L, beef extract 1.4g / L, peptone 4.0g / L, urea 0.8g / L, chlorine Sodium chloride 3.0g / L and potassium dihydrogen phosphate 3.0g / L are prepared to obtain the second-generation enrichment medium, and the pH value of the second-generation enrichment medium is adjusted to 6.4;

[0042] ②Collection of bacterial source samples: In Septemb...

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Abstract

The invention discloses a screening and domesticating method of microbial flora used for purifying water quality of gardens. The screening and domesticating method comprises the following steps: obtaining a bacterium source sample from sludge of water ponds of the gardens; preparing an enrichment culture medium according to nutritional requirements of efficient purification flora; adding the bacterium source sample into the enrichment culture medium and carrying out rapid enrichment culture to obtain a culture solution, so as to rapidly enrich a lot of flora, with high pertinence and high enrichment efficiency; utilizing a designed domesticating device and adding nutrient substances; carrying out domesticating treatment to obtain the microbial flora. The domesticating device has the advantages of simple structure, energy saving and high efficiency and high practicability; under a normal-temperature condition, the screened microbial flora can be domesticated through natural pressure difference, natural water temperatures and the like and a domesticating process is energy-saving and environmentally friendly; the method disclosed by the invention is economical, efficient, energy-saving and practical; after the screened microbial flora are domesticated, the microbial flora can be rapidly adapted to a water environment of the gardens; the efficient purification of sewage pond water of the water ponds of the gardens can be realized; the screening and domesticating method is ecologically safe, has no pollution and a high adaptive capacity, and is convenient for large-area application and popularization.

Description

technical field [0001] The invention belongs to the technical field of biological environment treatment, and in particular relates to a method for screening and domesticating microbial flora used for garden water purification. Background technique [0002] In recent years, with the continuous improvement of living standards, people are paying more and more attention to the natural environment of houses while improving their living conditions. Therefore, a large number of real estate projects have added landscaping and landscape water design to enhance the scenery of the community. However, at present, the waterscapes of many projects are located on the top of underground garages. Reinforced concrete structures are adopted to prevent water leakage. Most of the water bodies are closed and slow-flowing water bodies with static or poor fluidity. The self-purification ability is basically lost, so the ecological balance of the water body is very easy to be unbalanced. Especially...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12N1/00
CPCC12N1/00C12N1/36
Inventor 张松岳陶恺胜周波
Owner NINGBO JINGQI PROPERTY DEV CO LTD
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