Screening and domesticating method of microbial flora used for purifying water quality of gardens
A technology for microbial flora and water purification, which is applied in the methods of using microorganisms, biochemical equipment and methods, microorganisms, etc., and can solve the problems of complex breeding, long cycle, insufficient adaptability, and difficult operation.
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[0017] The method for screening and acclimating microbial flora used for garden water purification in Example 1 includes the following steps:
[0018] ①Preparation of enrichment medium: In 1L of sterile water, based on the volume of 1L of sterile water, add 1.4g / L of yeast extract, 5.0g / L of glucose, 3.0g / L of beef extract, and 6.9g of peptone. L, 1.1g / L urea, 4.8g / L sodium chloride, and 5.9g / L potassium dihydrogen phosphate to prepare a first-generation enrichment medium, and adjust the pH value of the first-generation enrichment medium to 6.4; 1L of sterile water, add yeast extract 0.9g / L, glucose 3.2g / L, beef extract 1.4g / L, peptone 3.6g / L, urea 0.6g / L, and chlorine based on the volume of the 1L sterile water. Sodium chloride 3.0g / L and potassium dihydrogen phosphate 2.9g / L were prepared to obtain a second-generation enrichment medium, and the pH value of the second-generation enrichment medium was adjusted to 5.2;
[0019] ②Bacterial source sample collection: In June, 100 mL o...
Example Embodiment
[0030] The method for screening and acclimating microbial flora for garden water purification in Example 2 includes the following steps:
[0031] ①Preparation of enrichment medium: In 1L of sterile water, add 1.5g / L of yeast extract, 5.3g / L of glucose, 3.1g / L of beef extract, and 7.2g / L of peptone based on the volume of 1L of sterile water. L, 1.2g / L urea, 5.0g / L sodium chloride and 6.0g / L potassium dihydrogen phosphate were prepared to obtain a first-generation enrichment medium, and the pH value of the first-generation enrichment medium was adjusted to 6.5; 1L of sterile water, add yeast extract 1.0g / L, glucose 3.3g / L, beef extract 1.3g / L, peptone 3.8g / L, urea 0.7g / L, and chlorine based on the volume of the 1L sterile water. Sodium chloride 3.2g / L and potassium dihydrogen phosphate 3.0g / L were prepared to obtain the second-generation enrichment medium, and the pH value of the second-generation enrichment medium was adjusted to 5.8;
[0032] ②Bacterial source sample collection: I...
Example Embodiment
[0040] The method for screening and acclimating microbial flora for garden water purification in Example 3 includes the following steps:
[0041] ①Preparation of enrichment medium: In 1L of sterile water, add 1.6g / L of yeast extract, 5.4g / L of glucose, 3.2g / L of beef extract, and 7.0g / of peptone based on the volume of 1L of sterile water. L, 1.1g / L urea, 5.1g / L sodium chloride, and 6.2g / L potassium dihydrogen phosphate to prepare a first-generation enrichment medium, and adjust the pH value of the first-generation enrichment medium to 6.6; 1L of sterile water, add yeast extract 1.1g / L, glucose 3.4g / L, beef extract 1.4g / L, peptone 4.0g / L, urea 0.8g / L, and chlorine based on the volume of the 1L sterile water. Sodium chloride 3.0g / L and potassium dihydrogen phosphate 3.0g / L were prepared to obtain the second-generation enrichment medium, and the pH value of the second-generation enrichment medium was adjusted to 6.4;
[0042] ②Bacterial source sample collection: In September, 200 mL...
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