Rhizobium sp. T3 and applications thereof in microbial degradation hydrogen sulfide

A technology for microbial degradation and Rhizobium, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc.

Active Publication Date: 2012-01-18
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Rhizobium sp. in the present invention is a kind of common bacterium, through retrieval patent and other relevant documents, have not yet found to utilize Rhizobium sp. to degrade H

Method used

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  • Rhizobium sp. T3 and applications thereof in microbial degradation hydrogen sulfide
  • Rhizobium sp. T3 and applications thereof in microbial degradation hydrogen sulfide
  • Rhizobium sp. T3 and applications thereof in microbial degradation hydrogen sulfide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1 Isolation, purification and identification of Rhizobium sp.T3

[0073] The final concentration of the inorganic salt medium of the present invention consists of: sodium thiosulfate 8.0g / L, glucose 0.2g / L, KH 2 PO 4 1.2g / L, K 2 HPO 4 1.2g / L, NH 4 Cl 0.4g / L, MgCl 2 ·6H 2 O 0.2g / L, ferric citrate 0.01g / L, solvent is water, pH 7.0.

[0074] Preparation of agar plate: sodium thiosulfate 8.0g / L, glucose 0.2g / L, KH 2 PO 4 1.2g / L, K 2 HPO 4 1.2g / L, NH 4 Cl 0.4g / L, MgCl 2 ·6H 2 O 0.2g / L, ferric citrate 0.01g / L, solvent is water, pH 7.0, add 15-20g of agar and heat to dissolve.

[0075] 1. Isolation and purification of strain T3

[0076] Rhizobium sp.T3 is a gram-negative bacterium domesticated and isolated from the activated sludge water sample of the wastewater treatment station of Zhejiang Huahai Pharmaceutical Factory. The specific steps are as follows:

[0077] Take 10mL of water samples (10% inoculum size) from the wastewater treatment station o...

Embodiment 2

[0096] Example 2 Rhizobium T3 (Rhizobium sp.T3) to different initial concentrations of H 2 Degradation performance test of S

[0097] (1) Slant culture: Inoculate Rhizobium T3 into the slant medium, cultivate at 30°C for 24 hours, and obtain slant bacteria; the final concentration of the slant medium consists of: yeast powder 5g / L, tryptone 10g / L, chlorine Sodium chloride 10g / L, agar 15g / L, solvent is water, pH value 7.0, sterilized at 121°C for 15min;

[0098] (2) Seed culture: use an inoculation loop to take an inoculation loop from the inclined-plane thalline and inoculate it into the seed medium, cultivate it at 30°C and 160r / min for 2 days, and obtain the seed liquid; the final concentration of the seed medium consists of: yeast powder 5g / L, tryptone 10g / L, sodium chloride 10g / L, agar 15g / L, solvent is water, pH value 7.0, sterilized at 121 ℃ for 15min;

[0099](3) Preparation of bacterial cell suspension: centrifuge the seed liquid at 12000r / min for 10min, discard the ...

Embodiment 3

[0104] Example 3 Rhizobium sp.T3 bacterial strain is to H at different temperatures 2 Degradation properties of S

[0105] (1) Slant culture: inoculate Rhizobium T3 into the slant medium, cultivate at 30° C. for 24 hours to obtain slant bacteria; the final concentration of the slant medium is composed of: yeast extract 5 g / L, tryptone 10 g / L, Sodium chloride 10g / L, agar 15g / L, solvent is water, pH value 7.0, sterilized at 121°C for 15min;

[0106] (2) Seed culture: use an inoculation loop to take an inoculation loop from the inclined-plane thalline and inoculate it into the seed medium, cultivate it at 30°C and 160r / min for 2 days, and obtain the seed liquid; the final concentration of the seed medium consists of: yeast powder 5g / L, tryptone 10g / L, sodium chloride 10g / L, agar 15g / L, solvent is water, pH value 7.0, sterilized at 121°C for 15min;

[0107] (3) Preparation of bacterial cell suspension: centrifuge the seed liquid at 12000r / min for 10min, discard the supernatant, ...

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Abstract

The invention discloses rhizobium sp. T3 which is preserved in China typical culture collection center, the preservation date is April, 2nd 2011, the preservation number is CCTCC No.M2011105, and the preservation address is Wuhan University in Wuhan, China. The application comprises the following step: by taking hydrogen sulfide as the only sulfur source and energy source, culturing the bacterial liquid obtained from the amplification culture of rhizobium sp.T3 selectively for 2-3 days at the temperature of 25-40 DEG C under an enclosed condition with the pH of 4.0-9.0, thus hydrogen sulfide is resolved, wherein the concentration of bacterial liquid cells is 200-300mg/L, and the initial concentration of hydrogen sulfide is 150-600ppm. The bacterial strain provided by the invention has efficient and high-tolerance hydrogen sulfide degradation capability, also can adapt to complex actual conditions, and has wide application prospects.

Description

(1) Technical field [0001] The invention relates to a new bacterial strain capable of degrading hydrogen sulfide—rhizobacillus T3, and its application in microbial degradation of hydrogen sulfide. (2) Background technology [0002] Hydrogen sulfide (H 2 S) is a colorless gas with an irritating and suffocating smell of rotten eggs. Inhalation of hydrogen sulfide by the human body can cause acute poisoning and chronic damage. Its mechanism of action is H 2 After S enters the blood, it combines with hemoglobin to generate irreducible sulfurized hemoglobin, and symptoms of poisoning occur. Long-term exposure to low concentrations of H 2 S presents with headaches, fatigue, memory loss, insomnia, chest pain, cough, nausea and diarrhea, and punctate keratitis. h 2 S concentration greater than 200×10 -6 g / m 3 When it is a dangerous value, it can completely paralyze the olfactory nerve. The concentration is greater than (700~1000)×10 -6 g / m 3 When the exposure occurs, coma a...

Claims

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Application Information

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IPC IPC(8): C12N1/20B01D53/84B01D53/52C12R1/41
CPCY02A50/20
Inventor 姜理英陈建孟王惠祥吴晓薇
Owner ZHEJIANG UNIV OF TECH
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