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Rhizobium and microbial inoculum, preparation method and application thereof

A technology of rhizobia and radiorhizobia, applied in the field of microorganisms, to achieve the effect of good potential application value

Active Publication Date: 2015-09-16
YUNNAN ACAD OF TOBACCO AGRI SCI +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And a kind of microbial agent that has multiple effects has no relevant reports

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  • Rhizobium and microbial inoculum, preparation method and application thereof
  • Rhizobium and microbial inoculum, preparation method and application thereof

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preparation example Construction

[0032] Rhizobium of the present invention is the preparation method of the inoculum of active ingredient, comprises plate culture, seed culture, fermentation, the preparation step of inoculum, specifically comprises:

[0033] A, plate culture: Radiation Rhizobium ( R. radiobacter ) A32 was inoculated on the plate medium, and cultured at a temperature of 26-30°C for 48-54 hours to obtain plate strains;

[0034] B. Seed culture: inoculate the plate strains into the liquid medium, and cultivate them on a shaker at a temperature of 26-30°C for 48-60 hours to obtain liquid-fermented seeds;

[0035] C. Fermentation: Inoculate the liquid fermented seeds into the fermenter according to the volume of 8~12% of the fermentation medium volume, cultivate at 28~32°C for 36~54h, the stirring speed is 170~190 r / min, and the ventilation volume is 60~100 %, obtain radiorhizobium A32 fermented liquid;

[0036] D. Bacterial agent preparation: add an adsorbent to the fermentation broth for adsor...

Embodiment 1

[0045] - with radiorhizobia ( R. radiobacter ) A32 Preparation of formulations as active ingredient

[0046] radiorhizobium cultured on nutrient broth plate R. radiobacter A32 was inoculated into a 250ml Erlenmeyer flask containing nutrient broth liquid culture fluid, cultured on a shaking table with a rotation speed of 100-110 rpm for 60 hours, and then transferred to a flask containing liquid culture fluid according to 10% of the inoculation amount. In the tissue culture orchid vase, after culturing for 72 hours on a shaker with a rotation speed of 100-110 rpm, it is adsorbed with peat with a particle size of 2 times the volume and a particle size of 1-5 mm to form a solid bacterial formulation.

[0047] The composition of the above-mentioned nutrient broth plate culture medium is: peptone 10.0g, beef powder 5.0g, sodium chloride 5.0g, agar powder 15.0g, distilled water 1000m, pH7.0-7.5. The composition of the nutrient broth culture solution is: peptone 10.0g, beef powder...

Embodiment 2

[0050] - with radiorhizobia ( R. radiobacter ) A32 Preparation of formulations as active ingredient

[0051] Take the radiorhizobium cultured on the nutrient broth plate ( R. radiobacter ) was inoculated into a tissue culture orchid vase with a nutrient broth liquid culture medium, cultivated on a shaking table with a rotation speed of 100-110 rpm for 60 hours, and then transferred to a liquid fermentation culture bottle with a 10% inoculation amount. In the CT5-2 automatic fermentation tank of liquid solution, the feeding amount is 3.5L, the fermentation time is 48 hours, and 80L of peat with a particle size of 1-5mm is used for adsorption to form a solid bacterial dosage form.

[0052] The composition of the above-mentioned nutrient broth plate culture medium is: peptone 10.0g, beef powder 5.0g, sodium chloride 5.0g, agar powder 15.0g, distilled water 1000m, pH7.0-7.5. The composition of the nutrient broth culture solution is: peptone 10.0g, beef powder 5.0g, sodium chlori...

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Abstract

The invention discloses a rhizobium and microbial inoculum, preparation method and application thereof. The rhizobium is a Rhizobium radiobacter A32, the preservation department of the rhizobium is the China General Microbiological Culture Collection Center (CGMCC), the preservation date of the rhizobium is November 21, 2014, and the preservation serial number is CGMCC, No.10031. The rhizobium belongs to the kingdom of bacteria, the phylum of mycetozoan, the class of alpha-mycetozoan, the order of rhizobia, the family of rhizobia and the genus of rhizobia. The rhizobium is microbial inoculum which has active components and is obtained after plate culture, seed culture and fermentation are conducted on the radioactive rhizobium. Bacillus is microbial inoculum which has active components and is prepared through plate culture, seed culture, fermentation and fungicide preparation. The microbial inoculum is used for phosphate solubilizing and potassium solubilizing of soil and increase of the amount of soil mineral nitrogen.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a rhizobia and its bacterial agent, preparation method and application. Background technique [0002] With the extensive application of chemical fertilizers in modern agricultural production, the output value and yield of crops have been greatly improved, but it has also led to a series of problems such as the reduction of soil organic matter, soil compaction, and land productivity decline. Soil microorganisms can promote the transformation of soil nutrients, activate soil nutrients, and increase nutrient supply. Soil microorganisms participate in various biochemical reactions of soil nutrient transformation, which are closely related to the supply and loss of soil nutrients. In soil, soil microorganisms are involved in processes such as mineralization and humification of organic matter, nitrification and denitrification of nitrogen, and effective use of phosp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/00C12R1/41
Inventor 段玉琪夏振远晋艳李庆平解燕龙伟任情谢晏芬
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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