36results about How to "Nodulation rate is high" patented technology

The invention relates to a (Bradyrhizobium sp.) LX-JX01 and application thereof. A preparation method of an inoculant for the (Bradyrhizobium sp.) LX-JX01 includes the steps of preparing a liquid DM culture medium, preparing a solid DM culture medium, activating a strain and culturing the rhizobium. The strain can be in nodulation and symbiosis with Dalbergia odorifera. When the inoculant for the Dalbergia odorifera rhizobium LX-JX01 is used for planting Dalbergia odorifera seedlings, nodulation number root nodules is increased by 3823% than a control group, fresh weight of the root nodules is increased by 1038%, dry weight of portions, above the ground, of plants is increased by 47.22%, and seedling height and plant stem diameter are increased by 1030% and 42.3% respectively, so that the inoculant can promote growing of Dalbergia odorifera. Consequently, the inoculant has wide application prospect in planting of artificial forest of Dalbergia odorifera.

The invention relates to a soybean slower-growing rhizobium with broad-spectrum nodulation characteristics, application thereof and a composite rhizobium agent prepared from the same, and belongs to the technical field of agricultural microorganisms. In order to solve the problems that the soybean nodule bacteria are low in nodulation rate and poor in nitrogen fixing effect, the invention providesa Japanese Bradyrhizobium japonicum HF25, the preservation number is CGMCC No.14857, the bacterial strain has high matching affinity, nodulation competitiveness and nodulation and nitrogen fixation capacity. The invention further provides the composite rhizobium agent which is prepared from Japanese Bradyrhizobium japonicum HF25 and Klebsiella oxytoca ASP-15, two kinds of bacteria are compounded,and the nodulation and nitrogen fixation capacity is significantly improved; the composite rhizobium agent is applied to soybean planting, compared with soybeans which are not applied with a microbial agent, the number of soybean nodules is increased by 170%, the number of pods per plant is increased by 37.3%, and the hundred-grain weight of soybeans is increased by 5.5%.

The invention provides a mesorhizobium kowhaii and Astragalus mongholicus rhizobium agent, a method and an application, and belongs to the technical field of microbial agents. The mesorhizobium kowhaii YS-1 provided by the invention has the preservation number of CGMCC No.20571, and has the characteristics of high nodulation rate and strong nitrogen fixation capability, can significantly improve the summarized nodulation number, effective nodulation number and nodulation weight of astragalus membranaceus, reduces the use amount of chemical nitrogen fertilizer in astragalus membranaceus planting, reduces the pollution of nitrogen fertilizer to the environment, and plays a significant role and has important and profound significance on production of green food and high yield, high efficiency and low consumption of agriculture.

The invention discloses a nitrogen-fixing bradyrhizobium japonicum strain resistant to saline-alkali soil and application of the bradyrhizobium japonicum strain. According to the invention, a strain of bradyrhizobium taihuae Dian strain is obtained through separation and is preserved in Guangdong Microbial Culture Collection Center on January 11, 2022, and the preservation number is GDMCC No: 62202. Researches show that the strain has the characteristics of high nodulation rate and strong nitrogen fixation capability, and can optimize the micro-ecological environment of alkaline soil crop root growth; the Dian strain is adopted to treat plants, soybean nodulation and nitrogen fixation can be effectively promoted, plant nitrogen nutrition is increased, plant growth is promoted, and the biomass, root nodule number and nitrogen content of soybean plants can be remarkably improved. The strain is especially suitable for cultivation and production of soybeans in stony desertification areas, expands a strain resource library of nitrogen-fixing rhizobium, is beneficial to promoting nodulation and nitrogen fixation of plants, and increases nitrogen nutrition of the plants.

The invention discloses a Chinese milk vetch microbial fertilizer. The Chinese milk vetch microbial fertilizer is prepared by mixing a solid biological fertilizer with mycorrhiza fungi in a weight ratio of 9 to 1, wherein the solid biological fertilizer is formed by fermenting a liquid bacterial liquid in a carrier substrate; and a bacterial strain of the liquid bacterial liquid is formed by mixing and inoculating bradyrhizobium and agrobacterium in mesorhizobium huakuii of the Chinese milk vetch. The invention further provides a preparation method of the Chinese milk vetch microbial fertilizer.

The invention relates to Mesorhizobium KDRM611 and the application of the Mesorhizobium KDRM 565. The Mesorhizobium KDRM611 is separated from Robinia pseudoacacia root nodule, contains ACC deaminase and can effective carry out nodulation and promote the growth of the Robinia pseudoacacia. The Mesorhizobium KDRM 611 strain is preserved in China Center for Type CultureCollection with the preservation number of CCTCC NO:M2012335. The Mesorhizobium KDRM 611 disclosed by the invention contains the ACC deaminase which can decompose ACC into alpha-ketobutyrate and NH3, reduce the level of synthesizing ethylene with plant cells, reduce an inhibition function of ethylene on rhizobium infection, improve the nodulation rate of rhizobium and the Robinia pseudoacacia and the level of symbiotic nitrogen fixation, provide high-level nitrogen for the growth of the Robinia pseudoacacia on non-fertilized barren wasteland, promote the growth of seedlings of the Robinia pseudoacacia and increase the biomass of mature the Robinia pseudoacacia, thereby realizing high yield of the mature Robinia pseudoacacia by using inoculation with low cost, and playing a role in Robinia pseudoacacia culture and forestation.

The invention relates to a mesorhizobium KDRM185 capable of being separated from an acacia root nodule, containing ACC deaminase, efficiently nodulating and promoting acacia growth and application thereof. A mesorhizobium KDRM185 strain is collected in CCTCC (China Center for Type Culture Collection), and the collection number is CCTCC No: M2012329. The mesorhizobium KDRM185 disclosed by the invention contains the ACC deaminase and can decompose the ACC into alpha-ketobutyrate and NH3, lower the level of plant cells on synthesizing ethylene, reduce the inhibiting effect of the ethylene on rhizobium infestation, enhance the nodulation rate and symbiotic nitrogen fixation level of rhizobia and acacia, provide high-level nitrogen for the growth of the acacia on unfertilized sterile wastelands, promote the growth of acacia seedlings and increase the biomass of acacia lumbers, thereby achieving the high yield of the acacia lumbers through low-cost inoculation investment and playing a role in acacia seedling culture and forestation.

The invention discloses a cassia rhizobium strain TXN1 and application thereof and belongs to the technical field of microorganism. The cassia rhizobium strain TXN1 is obtained by being separated andpurified from fresh nodules of cassia grasses and performing PCR (polymerase chain reaction) detection on nodA (nodulation protein A) gene as well as 16S rDNA (ribosomal deoxyribonucleic acid identification) molecular biological detection, determined to be a new strain of Bradyrhizobium and named as Tianyu-1. The cassia rhizobium strain TXN1 is preserved in China Center for Type Culture Collectionin April 10, 2018 with a preservation number of CCTCC NO: M2018191. Laboratory pot culture inoculation experiments prove that the cassia rhizobium strain TXN1 has the advantages of efficient nodulation and large nitrogen fixing capacity; inoculation of the cassia rhizobium strain TXN1 can significantly increase the number of root nodules, the root nitrogen fixing efficiency, the biomass and the plant nitrogen content of cassia grasses and further achieve the effects of increasing soil fertility and improving the ecological environment.

The invention discloses a rhizobium YZLH133 and application thereof. The strain is a new strain which is captured from the soil in Youxi District, Fujian Province through Longhua peanut variety, is separated and purified from fresh nodules, is determined to be Bradyrhizobium through phylogenetic analysis by testing part sequences of 16s rDNA of the strain, and is named Funong 3#. A laboratory hydroponic tieback test and a large field tieback experiment prove that the rhizobium YZLH133 disclosed by the invention has the characteristics of efficient nodulation and strong nitrogen fixing capacity; the biomass and the yield of peanuts can be improved by inoculating the rhizobium; and the rhizobium is applicable to acid soil in South China to serve as one of the measures for conventional cultivation of the peanuts.

The invention discloses chamaecrista bradyrhizobiumsp. WYS3R1 and application thereof and belongs to the technical field of microorganisms. A strain line is separated and purified from fresh chamaecrista grass nodule; nodA gene is detected by PCR; a novel strain line of Bradyrhizobium is determined by biological assay of 16S rDNA molecule and is named as Wuyuan 1. The chamaecrista bradyrhizobiumsp. WYS3R1 was preserved in China Center for Type CultureCollection on April 10th, 2018, with a preservation number of CCTCC NO:M2018190. The bonsai tieback test in a laboratory proves that the bradyrhizobiumsp. WYS3R1 disclosed by the invention has the characteristics of efficient nodulation and nitrogen fixation capabilities; by inoculating the bradyrhizobiumsp., nodule number, nodule nitrogen fixation efficiency and the biomass of chamaecrista grass and nitrogen content of plants can be obviously improved, and further the effects of culturing soil fertility and improving ecological environment are realized.

The invention discloses rhizobium YZM0144 and application thereof. The rhizobium YZM0144 is obtained by collecting rhizobium in soil of Youxi area of Fujian Province through a MO peanut novel variety,the rhizobium is separated and purified from fresh nodules, a 16s rDNA partial sequence of a strain is determined and identified as a novel strain of Bradyrhizobium by a phylogenetic analysis, and the rhizobium is named as Funong No. 4. The rhizobium YZM0144 proves that the rhizobium has the advantages of high-efficiency nodulation and strong nitrogen-fixing ability through laboratory hydroponicreturning experiments and field returning experiments, inoculation of the rhizobium can increase peanut biomass and yield, and the rhizobium can be applied to acid soil in South China as one of the measures for the routine cultivation of peanuts.

The invention relates to Mesorhizobium KDRM 565 and the application of the Mesorhizobium KDRM 565, wherein the Mesorhizobium KDRM 565 is separated from Robinia pseudoacacia root nodule, contains ACC deaminase and can be used for effective carrying out nodulation and promote growth of Robinia pseudoacacia. The Mesorhizobium KDRM 565 strain is preserved in China Center for Type CultureCollection with the preservation number of CCTCC NO:M2012334. The Mesorhizobium KDRM 565 disclosed by the invention contains ACC deaminase which can decompose ACC into alpha-ketobutyrate and NH3, reduce the level of synthesizing ethylene with plant cells, reduce an inhibition function of ethylene on rhizobium infection, improve the nodulation rate of rhizobium and Robinia pseudoacacia and the level of symbiotic nitrogen fixation, provide high-level nitrogen for the growth of the Robinia pseudoacacia on non-fertilized barren wasteland, promote the growth of seedlings of Robinia pseudoacacia and increase the biomass of mature Robinia pseudoacacia, thereby realizing the high yield the matureRobinia pseudoacacia by using inoculation with low cost, and playing a role in Robinia pseudoacacia culture and forestation.

The invention discloses rhizobium FAMB126 and application thereof. The rhizobium FAMB126 is obtained by collecting rhizobium in soil of Anxi area of Fujian Province through a peanut variety of MinhuaNo.8, the rhizobium is separated and purified from fresh nodules, a 16s rDNA partial sequence of a strain is determined and identified as a novel strain of Bradyrhizobium by a phylogenetic analysis, and the rhizobium is named as Funong No. 2. The rhizobium FAMB126 proves that the rhizobium has the advantages of high-efficiency nodulation and strong nitrogen-fixing ability through laboratory hydroponic returning experiments and field returning experiments, inoculation of the rhizobium can increase peanut biomass and yield, and the rhizobium can be applied to acid soil in South China as one of the measures for the routine cultivation of peanuts.