36results about How to "Nodulation rate is high" patented technology

The invention relates to Bradyrhizobium sp. LX‑JX01 and its application. The preparation method of the bradyrhizobium LX-JX01 bacterial agent of the present invention comprises the steps of liquid DM medium preparation, solid DM medium preparation, strain activation and rhizobia culture. The strain can nodulate symbiosis with Dalbergia balata. When Dalbergia japonica LX-JX01 bacterial agent of the present invention was used to plant Dalbergia japonica seedlings, its nodule nodule number increased by 3823% compared with the control, the fresh weight of nodules increased by 1038%, the dry weight of the aboveground part of the plant increased by 47.22%, and the seedling height and plant diameter are increased by 1030% and 42.3% respectively, therefore, the bacterial agent of the present invention can promote the growth of Dalbergia balsamicum. Therefore, the fungal agent of the present invention has broad application prospect in the plantation of Dalbergia balsamanii artificial forest.

The invention relates to mesorhizobium KDRM295 which is separated from acacia root nodule, contains ACC deaminase, and can efficiently nodulate and promote growth of the acacia, and an application thereof. The mesorhizobium KDRM295 is now preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCCNO: M2012331. The mesorhizobium KDRM295 contains ACC deaminase which can decompose ACC into alpha-ketobutyrate and NH3, reduce the level of plant cell for synthesizing ethylene, lighten the inhibition effect of ethylene to infection of the root nodule, improve the nodulation rate of root nodule and acacia and the level of symbiotic nitrogen fixation, provide high-level nitrogen for the growth of the acacia on the barren wasteland without fertilization, promote the growth of acacia seeding, and increase mature biomass for the acacia, thereby realizing high yield of the acacia mature with low inoculation investment, and playing a role of raising seeding and afforestation for acacia.

The invention discloses a rhizobia YZM0144 and its application. The strain is obtained from the rhizobia in the soil of Youxi area, Fujian Province through a new variety of MO peanut, and the rhizobia is separated and purified from fresh nodules, and the strain is of 16s rDNA partial sequence was determined, and through phylogenetic analysis, it was identified as Bradyrhizobium ( Bradyrhizobium ) of the new strain and named it Funong No. 4. The rhizobia YZM0144 of the present invention has the characteristics of high-efficiency nodulation and strong nitrogen fixation ability through laboratory hydroponic backgrafting tests and field backgrafting experiments. Inoculation of the rhizobia can increase peanut biomass and yield, and can be applied to acidic soil in South China , as one of the measures for conventional cultivation of peanuts.

The invention discloses a rhizobia FAMB126 and its application. The strain is obtained from the rhizobia in the soil of Anxi area, Fujian Province by Minhua No. 8 peanut variety, and the rhizobia is separated and purified from fresh nodules, and the strain of 16s The rDNA partial sequence was determined, and through phylogenetic analysis, it was determined to be Bradyrhizobium ( Bradyrhizobium ) of the new strain and named it Funong 2. The rhizobia FAMB126 of the present invention has been proved to have the characteristics of high-efficiency nodulation and strong nitrogen fixation ability through laboratory hydroponic backgrafting tests and field backgrafting experiments. Inoculation of the rhizobia FAMB126 can increase peanut biomass and yield, and can be applied to acidic soil in South China , as one of the measures for conventional cultivation of peanuts.

The invention relates to mesorhizobium KDRM495 and application thereof. The mesorhizobium KDRM495 is separated from acacia root nodule, contains ACC deaminase, and can efficiently nodulate and promotes acacia growth. A mesorhizobium KDRM495 strain is collected in CCTCC (China Center for Type Culture Collection), and the collection number is CCTCC No: M2012333. The mesorhizobium KDRM495 disclosed by the invention contains the ACC deaminase which can decompose ACC into alpha-ketobutyrate and NH3, lowers the level of synthesizing ethylene from plant cells, reduces the inhibiting effect of ethylene on rhizobium infestation, enhances the nodulation rate and symbiotic nitrogen fixation level of rhizobia and acacia, provides the high-level nitrogen for the growth of the acacia on unfertilized sterile wastelands, promotes the growth of acacia seedlings and increases the biomass of acacia lumbers, thereby achieving the high yield of the acacia lumbers through low-cost inoculation investment and playing a role in the aspect of acacia seedling culture and forestation.

The invention relates to a mesorhizobium KDRM185 capable of being separated from an acacia root nodule, containing ACC deaminase, efficiently nodulating and promoting acacia growth and application thereof. A mesorhizobium KDRM185 strain is collected in CCTCC (China Center for Type Culture Collection), and the collection number is CCTCC No: M2012329. The mesorhizobium KDRM185 disclosed by the invention contains the ACC deaminase and can decompose the ACC into alpha-ketobutyrate and NH3, lower the level of plant cells on synthesizing ethylene, reduce the inhibiting effect of the ethylene on rhizobium infestation, enhance the nodulation rate and symbiotic nitrogen fixation level of rhizobia and acacia, provide high-level nitrogen for the growth of the acacia on unfertilized sterile wastelands, promote the growth of acacia seedlings and increase the biomass of acacia lumbers, thereby achieving the high yield of the acacia lumbers through low-cost inoculation investment and playing a role in acacia seedling culture and forestation.

The invention discloses a rhizobium strain WYS3R1 and an application thereof, belonging to the technical field of microorganisms. The strain was isolated and purified from fresh nodules of the genus Cassia forage, detected by PCR nodA gene, and identified by 16S rDNA molecular biology, identified as Bradyrhizobium ( Bradyrhizobium ) of the new strain and named Wuyuan 1. It was deposited in the China Center for Type Culture Collection on April 10, 2018, and the deposit number is CCTCC NO: M2018190. The rhizobium WYS3R1 of the present invention has the characteristics of efficient nodulation and strong nitrogen fixation ability through the laboratory pot plant back-connection test, and inoculation of the rhizobium can significantly increase the number of nodules, the nitrogen-fixing efficiency of nodules, the biomass and plant nitrogen of the forage of the genus Cassia content, and then achieve the effect of fertilizing soil fertility and improving the ecological environment.

The invention discloses a method for promoting the growth of clover and / or increasing the yield of clover and the bacterial agent used therein. The method for promoting the growth of clover and / or increasing the yield of clover comprises the step of mixing the seeds of clover with the inoculum before sowing the soil; the inoculation contains the metabolites of Rhizobium clover or / and the Rhizobium clover ; The strain number of the Rhizobium clover bacteria is 92-29, and its preservation number in the General Microorganism Center of China Microbiological Culture Collection Management Committee is CGMCC No.15533. Clover rhizobia 92-29 of the present invention significantly promotes the growth of clover and significantly improves the output of clover under acidic soil conditions: improve the plant height, nodulation rate, root nodule number and / or root nodule weight of clover; increase the plant fresh weight and dry weight of clover Heavy, increases clover's fresh grass yield and dry grass yield. The invention has broad application prospects in the clover planting industry.

The invention discloses a strain of rhizobia TXN1 and its application, belonging to the technical field of microbes. The strain is isolated and purified from fresh root nodules of Cassia forage, and detected by PCR nodA gene, and identified by 16S rDNA molecular biology, identified as Bradyrhizobium ( Bradyrhizobium ), and named it Tianyu No. 1. It was deposited in the China Center for Type Culture Collection on April 10, 2018, and the deposit number is CCTCC NO: M2018191. The nitrogen-fixing bacterium strain TXN1 of the present invention proves that it has the characteristics of high-efficiency nodulation and strong nitrogen fixation ability through laboratory pot plant backgrafting experiments, and the inoculation of the rhizobia can significantly increase the number of nodules, nitrogen fixation efficiency of nodules, biomass and The nitrogen content of the plant can be increased to achieve the effect of fertilizing the soil and improving the ecological environment.

The invention discloses a kind of rhizobia AXLQ16 and its application. The strain is obtained from the rhizobia in the soil of Anxi area, Fujian Province by Minhua No. 11 peanut variety, the rhizobia is separated and purified from fresh nodules, and the The 16S rDNA partial sequence was determined, and through phylogenetic analysis, it was identified as the genus Rapid-growing Rhizobium ( Rhizobium ) of the new strain and named it Funong No. 1. The rhizobia AXLQ16 of the present invention has been proved to have the characteristics of high-efficiency nodulation and strong nitrogen fixation ability through laboratory hydroponic backgrafting tests and field backgrafting experiments. Inoculation of the rhizobia AXLQ16 can increase peanut biomass and yield, and can be applied to acidic soil in South China , as one of the measures for conventional cultivation of peanuts.

The invention relates to mesorhizobium KDRM319 and application thereof. The mesorhizobium KDRM319 is separated from acacia root nodule, contains ACC deaminase and can efficiently nodulate and promote acacia growth. A mesorhizobium KDRM319 strain is collected in CCTCC (China Center for Type Culture Collection), and the collection number is CCTCC NO.M2012332. The mesorhizobium KDRM319 disclosed by the invention contains the ACC deaminase which can decompose ACC into alpha-ketobutyrate and NH3, lowers the level of synthesizing ethylene from plant cells, reduces the inhibiting effect of ethylene on rhizobium infestation, enhances the nodulation rate and symbiotic nitrogen fixation level of rhizobia and acacia, provides the high-level nitrogen for the growth of the acacia on unfertilized sterile wastelands, promotes the growth of acacia seedlings and increases the biomass of acacia lumbers, thereby achieving the high yield of the acacia lumbers through low-cost inoculation investment and playing a role in the aspect of acacia seedling culture and forestation.