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Mesorhizobium KDRM185 and application thereof

A KDRM185, a technology of Brachyrhizobia, applied in the field of applied microbiology, can solve problems such as complex mechanisms, and achieve the effect of increasing biomass, improving nodulation rate and symbiotic nitrogen fixation, and promoting growth.

Active Publication Date: 2014-04-09
ZHONGYING CHANGJIANG INTERNATIONAL NEW ENERGY INVESTMENT CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mechanism of rhizobia regulating ACC deaminase is complex

Method used

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  • Mesorhizobium KDRM185 and application thereof
  • Mesorhizobium KDRM185 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] 1. Isolation, Purification and Preservation of Rhizobia

[0024] Select the large and full root nodules on the roots of robust black locust from the wild black locust in Lengji Town, Gucheng County, Hubei Province, carefully cut off the root nodules and some roots with scissors, and put 5-15 root nodules obtained from the same root of black locust into a container with dry discoloration Silicone and in a small tube covered with absorbent cotton. Then the collected nodules were fully soaked in sterile water for imbibition, soaked in 95% alcohol for 30 s, then sterilized with 0.1% mercury chloride for 5 min, and rinsed with sterile water for 6 times. Put them into a sterile 2-ml centrifuge tube after numbering, grind the root nodules with a sterile grinding rod, add 1 ml of sterile water with a pipette and suck and drain the homogenate 3 times to suspend the homogenate, and serially dilute the suspension 10 times, 100 times and 1000 times, and then pipette 100 μl of the ...

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Abstract

The invention relates to a mesorhizobium KDRM185 capable of being separated from an acacia root nodule, containing ACC deaminase, efficiently nodulating and promoting acacia growth and application thereof. A mesorhizobium KDRM185 strain is collected in CCTCC (China Center for Type Culture Collection), and the collection number is CCTCC No: M2012329. The mesorhizobium KDRM185 disclosed by the invention contains the ACC deaminase and can decompose the ACC into alpha-ketobutyrate and NH3, lower the level of plant cells on synthesizing ethylene, reduce the inhibiting effect of the ethylene on rhizobium infestation, enhance the nodulation rate and symbiotic nitrogen fixation level of rhizobia and acacia, provide high-level nitrogen for the growth of the acacia on unfertilized sterile wastelands, promote the growth of acacia seedlings and increase the biomass of acacia lumbers, thereby achieving the high yield of the acacia lumbers through low-cost inoculation investment and playing a role in acacia seedling culture and forestation.

Description

technical field [0001] The invention belongs to the field of applied microbiology, and in particular relates to a bradyrhizobium KDRM185 isolated from root nodules of Robinia pseudoacacia, which has ACC deaminase and can efficiently form nodules and promote the growth of Robinia pseudoacacia, and an application thereof. The strain has been preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 2012329, and the strain name is Bradyrhizobium KDRM185. Background technique [0002] Some prokaryotic microorganisms in nature can synthesize nitrogenase, which can convert N in the air to 2 reduced to NH 3 . The nitrogen fixed by nitrogen-fixing microorganisms accounts for about 65% - 70% of the combined nitrogen on the surface. Among them, the symbiotic system of rhizobia and leguminous plants has the strongest nitrogen fixation ability, accounting for more than 65% of the biological nitrogen fixation. [0003] It is a classic ex...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01G1/00C12R1/41
Inventor 李江川李万里张继泰
Owner ZHONGYING CHANGJIANG INTERNATIONAL NEW ENERGY INVESTMENT CO LTD
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