Soybean slower-growing rhizobium with broad-spectrum nodulation characteristics, application thereof and composite rhizobium agent prepared from same

A technology of bradyrhizobium and compound rhizobia, applied in the field of agricultural microorganisms, can solve the problems of poor nitrogen fixation effect and low nodulation rate of soybean rhizobia, achieve good affinity, improve nodulation and nitrogen fixation ability, and achieve nodulation powerful effect

Inactive Publication Date: 2019-05-10
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of low nodulation rate and poor nitrogen fixation effect of soybean rhizobia, the present invention provides a strain of soybean bradyrhizobium with broad-spectrum nodulation characteristics and its application and a composite rhizobia agent prepared therefrom

Method used

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  • Soybean slower-growing rhizobium with broad-spectrum nodulation characteristics, application thereof and composite rhizobium agent prepared from same
  • Soybean slower-growing rhizobium with broad-spectrum nodulation characteristics, application thereof and composite rhizobium agent prepared from same
  • Soybean slower-growing rhizobium with broad-spectrum nodulation characteristics, application thereof and composite rhizobium agent prepared from same

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Soybeans in the growth stage were collected from soybean planting fields in Linkou, Heilongjiang, and a soybean rhizobia strain was obtained from the nodules of soybean roots, which was coded as HF25. Refer to the eighth edition of "Berger Bacteria Identification Manual" and "Common Bacterial System Identification Manual" to carry out morphological characteristics, physiological and biochemical characteristics and molecular biological identification of the strain, the results are as follows:

[0023] (1) Morphological characteristics:

[0024] Observed under a microscope, HF25 rhizobium belongs to Gram-negative bacteria, the bacteria are short rod-shaped, without spores, and the bacteria are slender. figure 1 ; HF25 rhizobia grow slowly on the mannitol medium, and the colonies are white protrusions, round, shiny, opaque, creamy, with a small amount of colloid formation, and the colony morphology is shown in figure 2 .

[0025] (2) Physiological and biochemical charac...

Embodiment 2

[0031] This embodiment has measured the influence of culture medium pH value on the growth of HF25 rhizobia:

[0032] Using aseptic operation, use an inoculation loop to pick up 1 ring of HF25 rhizobium slant culture and inoculate it into a conical flask containing 100ml of YM liquid medium. Place the conical flask at 30°C and rotate at 150r / min, and shake it for 48 hours. , to obtain the seed solution. The pH value of the culture medium in the test tube containing 5mLYM medium was adjusted to 4.0, 5.0, 6.0, 7.0, 8.0, 9.0 and 10.0 respectively, and 0.1ml HF25 seed solution was inoculated in each test tube, and each treatment was repeated 3 times. The culture test tubes were placed in a shaking incubator at 30°C for 3 days, and the OD value of each fermentation broth at a wavelength of 420 nm was measured. For different treatments, the medium without inoculation was used as the CK control, and the average OD value of each repetition was taken, and the results are shown in Tabl...

Embodiment 3

[0037] This embodiment has measured the influence of culture temperature on the growth of HF25 rhizobia:

[0038]Using aseptic operation, use an inoculation loop to pick up 1 ring of HF25 rhizobium slant culture and inoculate it into a conical flask containing 100ml of YM liquid medium. Place the conical flask at 30°C and rotate at 150r / min, and shake it for 48 hours. , to obtain the seed solution. Inoculate 0.1ml of HF25 rhizobia seed solution in each test tube containing 5ml of YM medium, the pH value of YM medium is 7.0, and place the inoculated test tubes at 15°C, 20°C, 25°C, 28°C, and 30°C respectively , 35°C and 40°C culture temperature for 2 days, 3 repetitions for each treatment, measure the OD value of each fermentation broth at a wavelength of 420nm, each treatment uses the non-inoculated culture medium as the CK control, and takes the average OD of each repetition values, and the results are shown in Table 2.

[0039] Table 2

[0040]

[0041] It can be seen f...

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Abstract

The invention relates to a soybean slower-growing rhizobium with broad-spectrum nodulation characteristics, application thereof and a composite rhizobium agent prepared from the same, and belongs to the technical field of agricultural microorganisms. In order to solve the problems that the soybean nodule bacteria are low in nodulation rate and poor in nitrogen fixing effect, the invention providesa Japanese Bradyrhizobium japonicum HF25, the preservation number is CGMCC No.14857, the bacterial strain has high matching affinity, nodulation competitiveness and nodulation and nitrogen fixation capacity. The invention further provides the composite rhizobium agent which is prepared from Japanese Bradyrhizobium japonicum HF25 and Klebsiella oxytoca ASP-15, two kinds of bacteria are compounded,and the nodulation and nitrogen fixation capacity is significantly improved; the composite rhizobium agent is applied to soybean planting, compared with soybeans which are not applied with a microbial agent, the number of soybean nodules is increased by 170%, the number of pods per plant is increased by 37.3%, and the hundred-grain weight of soybeans is increased by 5.5%.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a soybean bradyrhizobium strain with broad-spectrum nodulation characteristics, its application and a composite rhizobia agent prepared therefrom. Background technique [0002] Soybean is an important food crop and oil crop, and its absorption of nitrogen is relatively high. The production of 100kg of soybean absorbs about 6.5-8.5kg of nitrogen. Artificial inoculation of rhizobia is an effective measure to enhance soybean nodulation and nitrogen fixation, increase nitrogen nutrition, and increase soybean yield. At present, in major soybean exporting countries such as the United States, Brazil and Argentina, soybean nitrogen fertilizer is mainly provided by inoculating soybean rhizobia to form nodules. More than 30% to 65% of the planting area, or even 100% of the planting area, is only inoculated with soybean rhizobia. Biological nitrogen fixation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G22/40C12R1/01C12R1/22
Inventor 牛彦波吴晧琼安琦殷博曹亚彬王笑庸原韬
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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