A kind of broad bean rhizobium strain bga2-2 and its application
A broad bean and root nodule technology, applied in the field of microorganisms, can solve the problems of weakly soluble organic phosphorus and large differences in phosphorus dissolving ability, and achieve the effects of strong symbiotic nitrogen fixation ability, good affinity and strong stress resistance
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Embodiment 1
[0016] Isolation, purification and preservation of embodiment 1 rhizobia
[0017] From the broad beans planted on the yellow loam soil of Linshui County, Guang'an City, Sichuan Province, select the large and plump red root nodules on the taproots of robust plants, scrub the nodules clean, pick off part of the root bark, dry them with paper and put them in a container. In a small tube with anhydrous calcium chloride and covered with absorbent cotton. In the laboratory, the collected root nodules were soaked and swelled with sterile water, treated with 95% ethanol for 5 minutes, then sterilized with 0.1% m / v mercury chloride surface for 5 minutes, and then rinsed with sterile water for 6 times. In the case of bacterium operation, a single root nodule was clamped and added to the YMA medium (mannitol 10g, yeast powder 0.8g, KH 2 PO 4 0.25g, MgSO 4 .7H 2 O0.2g, CaCl 2 .6H 2 O 0.1g, NaCl 0.1g, sodium molybdate (1%) 2ml, boric acid (1%) 2ml, Congo red (1%) 2.5ml, agar 18-20g,...
Embodiment 2
[0020] Back grafting and matching test of embodiment 2 rhizobia
[0021] The broad bean variety used for the backgrafting test of rhizobia was "big white broad bean". It was carried out in a light room (temperature control 22-24°C, light intensity 2700-3000 lux, sunshine time 14hr), and harvested 46 days after planting. After successful backgrafting with "Big White Broad Bean", the matching affinity test with other main planted broad bean varieties ("Chenghu 14", "Chenghu 15", "Panzhihua Broad Bean") was carried out, which was also carried out by hydroponics. Harvest after 41 days of culture in the above light chamber. Regularly supplement sterile nitrogen-free nutrient solution. Fast-growing broad bean rhizobia Bga2-2 is formed into different combinations with the above-mentioned broad bean varieties, and the hydroponic device adopts a 250ml narrow-necked bottle (glass infusion bottle for hospital use), and the plant of the same variety that is not inoculated with the broad ...
Embodiment 3
[0030]The stress resistance of rhizobia Bga2-2 described in embodiment 3
[0031] The stress resistance of the rhizobia Bga2-2 was mainly determined by acid and alkali resistance, salt tolerance and growth in the greenhouse. The YMA medium was used as the basal medium, and the YMA plate cultured at pH 7 and 28°C for 7 days was used as the positive control. The above-mentioned YMA slant culture of Bga2-2 was scraped and washed with sterile water for use. The point inoculation method was adopted and repeated three times. The YMA medium was used as the basic medium for the determination of acid and alkali resistance. The pH value was adjusted with HCl and NaOH. The pH value was 4.0, 5.0, 6.0, 8.0, 9.0, 10.0, and 11.0. The determination of salt tolerance is also based on YMA medium, and the strains are spot-inoculated on a plate containing NaCl, and the mass volume fraction of NaCl is 0.2%, 0.4%, 0.6%, 1.0%, 1.5%, 2.5% , 3.5% and 4.5%. The plates for acid and alkali resistance ...
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