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A kind of broad bean rhizobium strain bga2-2 and its application

A broad bean and root nodule technology, applied in the field of microorganisms, can solve the problems of weakly soluble organic phosphorus and large differences in phosphorus dissolving ability, and achieve the effects of strong symbiotic nitrogen fixation ability, good affinity and strong stress resistance

Inactive Publication Date: 2020-06-16
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Shi Shangli (2007) conducted research on more than 730 strains of Medicago rhizobia, and only 10 strains of rhizobia with strong ability to secrete IAA were screened from these 29 strains, and the phosphorus-dissolving ability of these 29 strains was measured, and it was found that all The strains did not have the ability to dissolve inorganic phosphorus. The 29 Rhizobia meliloti could dissolve organic phosphorus, but the phosphorus-dissolving ability was quite different. Only 8 strains had strong ability to dissolve organic phosphorus, and the rest had weak or weak ability to dissolve organic phosphorus. Organophosphate capacity
[0006] At present, there are no relevant reports on the screening application of high-efficiency rhizobia in broad bean except Qinghai, Gansu (Diao Zhimin, 2002; Han Mei et al., 2010; Wang Wenli et al., 2010) and Hebei (Wang Rui, 2016). other reports

Method used

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  • A kind of broad bean rhizobium strain bga2-2 and its application
  • A kind of broad bean rhizobium strain bga2-2 and its application
  • A kind of broad bean rhizobium strain bga2-2 and its application

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Experimental program
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Effect test

Embodiment 1

[0016] Isolation, purification and preservation of embodiment 1 rhizobia

[0017] From the broad beans planted on the yellow loam soil of Linshui County, Guang'an City, Sichuan Province, select the large and plump red root nodules on the taproots of robust plants, scrub the nodules clean, pick off part of the root bark, dry them with paper and put them in a container. In a small tube with anhydrous calcium chloride and covered with absorbent cotton. In the laboratory, the collected root nodules were soaked and swelled with sterile water, treated with 95% ethanol for 5 minutes, then sterilized with 0.1% m / v mercury chloride surface for 5 minutes, and then rinsed with sterile water for 6 times. In the case of bacterium operation, a single root nodule was clamped and added to the YMA medium (mannitol 10g, yeast powder 0.8g, KH 2 PO 4 0.25g, MgSO 4 .7H 2 O0.2g, CaCl 2 .6H 2 O 0.1g, NaCl 0.1g, sodium molybdate (1%) 2ml, boric acid (1%) 2ml, Congo red (1%) 2.5ml, agar 18-20g,...

Embodiment 2

[0020] Back grafting and matching test of embodiment 2 rhizobia

[0021] The broad bean variety used for the backgrafting test of rhizobia was "big white broad bean". It was carried out in a light room (temperature control 22-24°C, light intensity 2700-3000 lux, sunshine time 14hr), and harvested 46 days after planting. After successful backgrafting with "Big White Broad Bean", the matching affinity test with other main planted broad bean varieties ("Chenghu 14", "Chenghu 15", "Panzhihua Broad Bean") was carried out, which was also carried out by hydroponics. Harvest after 41 days of culture in the above light chamber. Regularly supplement sterile nitrogen-free nutrient solution. Fast-growing broad bean rhizobia Bga2-2 is formed into different combinations with the above-mentioned broad bean varieties, and the hydroponic device adopts a 250ml narrow-necked bottle (glass infusion bottle for hospital use), and the plant of the same variety that is not inoculated with the broad ...

Embodiment 3

[0030]The stress resistance of rhizobia Bga2-2 described in embodiment 3

[0031] The stress resistance of the rhizobia Bga2-2 was mainly determined by acid and alkali resistance, salt tolerance and growth in the greenhouse. The YMA medium was used as the basal medium, and the YMA plate cultured at pH 7 and 28°C for 7 days was used as the positive control. The above-mentioned YMA slant culture of Bga2-2 was scraped and washed with sterile water for use. The point inoculation method was adopted and repeated three times. The YMA medium was used as the basic medium for the determination of acid and alkali resistance. The pH value was adjusted with HCl and NaOH. The pH value was 4.0, 5.0, 6.0, 8.0, 9.0, 10.0, and 11.0. The determination of salt tolerance is also based on YMA medium, and the strains are spot-inoculated on a plate containing NaCl, and the mass volume fraction of NaCl is 0.2%, 0.4%, 0.6%, 1.0%, 1.5%, 2.5% , 3.5% and 4.5%. The plates for acid and alkali resistance ...

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Abstract

A faba bean rhizobium strain Bga2‑2 and application thereof, the strain is isolated and purified from fresh faba bean nodules, and belongs to Rhizobium anhuiense strain. The strain was deposited in the Chinese Type Culture Collection Center of Wuhan University on October 23, 2017, and the preservation number is: CCTCC NO: M 2017613. The rhizobium strain Bga2-2 of the present invention is an excellent broad-spectrum faba bean rhizobia with strong symbiotic nitrogen fixation ability, wide adaptability to Sichuan broad bean varieties, secretion of IAA, ability to dissolve inorganic phosphorus and organic phosphorus, and strong stress resistance The strain has a good matching affinity with the main varieties of broad beans planted in Sichuan. In the production of different varieties of broad beans in Sichuan, no nitrogen fertilizer and inoculation with Bga2‑2 increased the yield of broad beans by more than 31.8%, and the difference from the control without inoculation reached a significant level.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a broad bean rhizobium strain Bga2-2 and its application. Background technique [0002] China is the largest broad bean producer, with dry broad bean production accounting for 35% of the world (FAO2014). It is an important agricultural product resource for my country's traditional foreign trade, and its export volume ranks second among the country's miscellaneous grains (Liu Yujiao et al., 2002). The sown area of ​​broad beans in Sichuan Province is about 300,000 hectares, second only to Yunnan, with a total output of 600 million kilograms (Yang Wuyun, 2003). Douban in Pixian County, Sichuan, which uses broad beans as raw materials, is well-known throughout the country and is in great demand. The annual demand for broad beans reaches 120,000 tons, but now it mainly relies on imports from other places. In addition to harvesting dry broad beans, fresh broad beans can also be harveste...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00A01C1/06C12R1/41
CPCA01C1/06A01N63/00C12N1/205C12R2001/41
Inventor 陈远学徐开未周元全紫曼陈沁园吴永成蒋帆王佳锐唐巧奇
Owner SICHUAN AGRI UNIV
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