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A rhizomatous strain of pea sp. vs21-2 and its application

A technology of arrowhead pea and rhizobia, applied in the field of microorganisms, to achieve the effects of obvious increase in yield, strong nitrogen fixation ability, and strong stress resistance

Inactive Publication Date: 2020-06-02
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on high-efficiency rhizobia suitable for Sichuan ecological environment is almost blank

Method used

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  • A rhizomatous strain of pea sp. vs21-2 and its application
  • A rhizomatous strain of pea sp. vs21-2 and its application
  • A rhizomatous strain of pea sp. vs21-2 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Isolation, Purification and Preservation of Rhizobium Arrowhead Pea Rhizobium VS21-2

[0016] From the wild arrowhead peas collected in Xiangshui Village, Yanjiang Town, Yanjiang District, Ziyang City, Sichuan Province, select the large and full red root nodules on the main roots of robust plants, wash the root nodules, and take part of the root bark for surface disinfection: use 95% Soak in ethanol for 5 minutes to eliminate surface tension, then sterilize the surface with mercury chloride (0.1% m / v) for 5 minutes, and finally rinse with sterile water for 6-8 times. Put the root nodules on a sterilized white porcelain plate under a sterile environment, clip the root nodules with a bamboo stick and smear them on the plate of YMA medium, perform streaking and purification, and culture in a 28°C incubator. YMA medium formula: KH 2 PO 4 0.25g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 ·6H 2 O 0.1g, NaCl 0.1g, ammonium molybdate (1%) 2mL, boric acid (1%) 2mL, Congo red (...

Embodiment 2

[0019] Example 2 The backgrain test of rhizobia

[0020] The backgrafting test of rhizobia uses the sand culture method, and the species of Arrowhead pea used in the test is North Sichuan Arrowhead pea, which comes from Xiangshui Village, Yanjiang Town, Yanjiang District, Ziyang City, Sichuan. Carry out in the light room (temperature control 22~24 ℃, light intensity about 2800 lux, sunshine time 14h), plant and harvest 46 days. Regularly supplement the sterilized nitrogen-free nutrient solution, inoculate the rhizobia VS21-2 into the sand culture device, the sand culture device uses a 300mL plastic cup, and use vermiculite as the substrate, so as not to inoculate the rhizobia VS21-2 The plants of the same variety as the control CK, each treatment was repeated three times. After harvesting, the nodule number and dry weight of the plants were used to evaluate the inoculation effect of Rhizobia VS21-2.

[0021] (1) Bacterial liquid culture: inoculate Rhizobium spp. VS21-2 in YM...

Embodiment 3

[0031] Example 3 The Stress Resistance of Pea Rhizobium VS21-2

[0032] The stress resistance of Rhizobium spp. VS21-2 was mainly tested for salt tolerance, acid and alkali resistance. The YMA medium was used as the basal medium, and the YMA plate cultured at pH 7 and 28°C for 7 days was used as the positive control (CK). Scrape and wash the above-mentioned YMA slant culture of Rhizobium argentina VS21-2 with sterile water to make a bacterial suspension for use. The spot inoculation method was used, and each treatment was repeated 3 times. Plates for acid and alkali resistance and salt resistance tests were all cultured at 28°C for 3 days, and the results were observed and recorded.

[0033] (1) Medium formula for acid and alkali resistance determination: take YMA medium as the basic medium, adjust the pH value with HCl and NaOH, so that the pH value of the medium is 4.0, 5.0, 6.0, 8.0, 9.0, 10.0, 11.0 .

[0034] (2) Medium formula for salt tolerance determination: take th...

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Abstract

The invention discloses a pea rhizobium strain VS21‑2 and application thereof. The strain is isolated and purified from fresh pea rhizobium and belongs to Rhizobium sophorae strain. The strain was deposited in the Chinese Type Culture Collection Center of Wuhan University on October 23, 2017, and the preservation number is: CCTCC NO: M 2017608. The pea rhizobia VS21-2 described in the present invention is applied to the production of peas in Sichuan. It is a kind of symbiotic nitrogen-fixing ability, which has an obvious effect on increasing the yield of peas. It also has the ability to secrete IAA, dissolve phosphorus, dissolve The excellent rhizobium strain of Arrowhead pea with strong potassium ability and stress resistance can increase the fresh grass yield of Arrowhead pea by more than 66.7%, and the fresh root yield by 73% without applying nitrogen fertilizers or other fertilizers and only inoculating rhizobia VS21‑2 .

Description

technical field [0001] The invention relates to the field of microbes, in particular to a strain VS21-2 of Pisa rhizobium and application thereof. Background technique [0002] Arrowhead pea (Vicia sativa L.), also known as Vicia sativa L., belongs to a cultivar of the genus Vetaceae of the family Leguminosae. It has a large main root, shallow penetration into the soil, many root nodules, and arrow-shaped leaves, so it is named Arrowhead Pea. Pea, which originated in southern Europe and western Asia, was introduced into my country for planting and cultivation in the 1940s. The planting range in my country reached 29 provinces (regions) including Qinghai, Jiangsu, Hunan, and Taiwan (Hong Ruxing et al., 1985). Arrowhead pea, as an excellent feed and green manure dual-purpose crop, has high nutritional value, tender stems and branches, more leaves, and good palatability. It is a relatively common green manure germplasm resource used in production (Lu Binglin et al., 2015; Zhao H...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/41
CPCC12N1/205C12R2001/41
Inventor 陈远学徐开未彭丹吴永成周元胡斐杨云飞
Owner SICHUAN AGRI UNIV
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