62results about How to "Strong nitrogen fixation ability" patented technology

The invention discloses bio-organic fertilizer, and belongs to the technical field of organic fertilizer. The proportion by weight of all raw materials of the bio-organic fertilizer is dried chicken manure : edible mushroom : compound microbial agent : corn flour or rice bran : functional strain = 110-140 : 390-360 : 0.1-0.5 ; 0.1. The compound microbial agent comprises mould, bacillus, lactic acid bacteria, actinomycetes and saccharomycetes. The strains are efficient viable bacteria. Each gram of the compound microbial agent comprises the number of the mould ranges from 5 hundred million to 8 hundred million, the number of the efficient viable bacteria of bacillus ranges from 12 hundred million to 15 hundred million, and the number of the actinomycetes ranges from 10 hundred million to 12 hundred million and the saccharomycetes of 15 hundred million to 18 million. According to the bio-organic fertilizer, the chicken manure and mushroom dregs serve as main materials, and the high-quality organic fertilizer can be fermented quickly by adding the compound microbial agent and the functional strain.

The invention relates to a purple heart pitaya planting and a cultivating method. The method comprises the steps that 1, seeding stage management: at the early stage of characterization , one time of watering is conducted every 2 to 3 days, and timely drainage is conducted in rainy season; 2, at the initial planting period, emergency crops can be interplanted in a new orchard. Sod culture is adopted to the cultivating management once the pediciel of the pitaya climbs to the top of a pillar. Ridge plowing and root covering are conducted according to the root baring situation after raining, and ridge plowing to protect seeding is conducted in winter in the new orchard; 3, scientific fertilizer and water index; 4, pruning; 5, flower and fruit thinning and fruit bagging; 6, pest control: priority is given to the agricultural, physical and biological control, and chemical control is supplemented; 7, reaping and marketing timely. According to the purple heart pitaya planting and cultivating method, three-dimensional management is adopted into the planting of the purple heart pitaya, the production management is convenient, the taste of the purple heart pitaya is good, and the yield is high.

The invention provides a compound photosynthetic bacteria preparation for enhancing the phytoremediation for heavy metal pollution of soil and a preparation method thereof. The high concentration compound photosynthetic bacteria preparation of thiocapsa roseopersicina and rhodopseudomonas capsulata is adopted through the step that thiocapsa roseopersicina cultures in purple sulfur photosynthetic bacteria and rhodopseudomonas capsulate cultures in purple non-sulfur photosynthetic bacteria are subjected to such culture steps as seed activation, seed culture and anaerobic fermentor irradiation culture. The compound photosynthetic bacteria preparation is used for enhancing the phytoremediation for heavy metal pollution of soil, thiocapsa roseopersicina as sulfur-oxidizing bacteria can be used for oxidizing relatively stable sulfides with heavy metals, thereby promoting sulfur cycle of soil, reducing the PH value of soil and improving the activity of heavy metals; the thiocapsa roseopersicina and rhodopseudomonas capsulata as bacterial manure can promote the conversion of soil substances, improve the soil structure and soil fertility, promote plant growth and improve the biomass of plants enriched with heavy metals, thereby enhancing the efficiency of phytoremediation for heavy metals in soil.

The invention discloses a degraded soil improving method with stylosanthes guianensis as ecological forage. The JinshaRiver dry-hot valley degraded soil is chosen to be treated with the method. According to the method, thoroughly decomposed farmyard manure and calcium superphosphate are applied during soil preparation, stylosanthes guianensis seedlings are transplanted in the rainy season, urea is further applied for one to two times when the stylosanthes guianensis seedlings germinate, timely drainage is guaranteed in the rainy season and the soil water content is limited below 15%, timely watering is guaranteed in the arid season and the soil water content is kept above 3%, 50% carbendazim wettable powder is used every year to prevent the occurrence of anthracnose, and 20% acetamiprid powder 3000 times liquid is used to kill aphids or armyworms in a spraying mode. According to the ecological characteristic of the Jinsha River dry-hot valley degraded soil, the method fully utilizes characteristics, such as drought-enduring, growing fast, cutting-enduring, strong nitrogen fixing capacity, strong adaptability and extensive management, of the stylosanthes guianensis, soil organic matter, the total nitrogen and available phosphorus content, and soil water contents in soil layers of 0-20 cm and 20-40 cm in the arid season are greatly improved.

The long-acting composite plant fertilizer consists of green material including foxtail-like sophora herb, alfalfa, plant ash, plant oil residue and adhesive and based on the production of farmyard manure. Its production includes the processes of drying, crushing, sieving, mixing, extrusion, pelletizing, and stoving. Field experiment shows that it has obvious yield increasing effect on rice, wheat, corn, melons and fruits and can reduce plant diseases and insect pests effectively.

The invention discloses rhizobium having high-efficiency phosphate-dissolving capability and an application thereof. The invention firstly discloses the rhizobium having high-efficiency phosphate-dissolving capability, and a microbe preservation number is CGMCC No.14614. The separated rhizobium bacterial strain has strong phosphate-dissolving capability, and has strong nitrogen-fixation capability, and has good nitrogenase activity under drought stress. Under phosphorus deficiency condition, the rhizobium has obvious growth-promoting capability on plant, and can obviously increase the output of medicago sativa. The invention also discloses an application of the rhizobium for increasing the leguminous plants output, increasing the phosphor absorption by the leguminous plants, and increasingthe nitrogen fixation efficiency of the leguminous plants. The invention also discloses an application of the rhizobium in preparation of a phosphate-dissolving bacterial manure or a nitrogen-fixation bacterial manure. The separated rhizobium having high-efficiency phosphate-dissolving capability has an important application prospect for increasing the output of the leguminous plant medicago sativa.

The invention discloses an efficient nitrogen-fixing alfalfa rhizobium strain. The strain has a collection name of Sinorhizobium meliloti 729-2-4 and is collected in CGMCC on October 10, 2014 and has a collection number of CGMCC No. 9755. The strain is a rhizobium strain which can establish good symbiotic relationship with alfalfa and is strong in nitrogen-fixing capability and the planting yield of the alfalfa can be effectively increased. Simultaneously, the invention also provides a method for screening a molecular marker of the rhizobium strain. The method is scientific, reasonable and simple and easy to operate and the method can be used for more accurately screening the efficient nitrogen-fixing rhizobium strain which is applicable to local climate and soil and establishes good symbiotic relationship with alfalfa.

The invention relates to the technical field of medicinal plant cultivation, in particular to a Ganoderma lucidum facility cultivation method. The Ganoderma lucidum facility cultivation method includes: feeding water to lands in Ganoderma lucidum cultivation sheds after the Ganoderma lucidum is harvested, scattering lime nitrogen and/or slaked lime to watered lands, removing sun-shading systems ofthe sheds, retaining farm films, sealing the Ganoderma lucidum cultivation sheds to perform shed closing; performing deep ploughing on soil in the sheds after the sheds are closed for 7-21 days; performing chlorine dioxide neutralization and disinfection, opening the farm films, scattering seeds of Astragalus sinicus and/or ryegrass in the Ganoderma lucidum cultivation sheds after the soil is dry, and allowing the Astragalus sinicus and/or ryegrass to naturally grow; and performing arrangement on next Ganoderma lucidum cultivation after performing deep ploughing on the soil where the Astragalus sinicus and/or ryegrass is planted. The Ganoderma lucidum facility cultivation method can reconstruct a soil micro-ecosystem by performing watering, disinfection, deep ploughing, and plant fertilization on the Ganoderma lucidum cultivation sheds, and can effectively solve the problem of successive cropping obstacles of the Ganoderma lucidum.

The invention provides a disease prevention plantation method for navel orange and belongs to the field of agricultural planting. The method comprises following steps: selecting soil which is suitable for the growth of navel orange and performing land preparing; selecting proper navel orange scion, stocks and interstock for grafting; after survival, regularly applying base fertilizer prepared from self-made organic fertilizer, nitrogenous fertilizer, phosphate fertilizer and potash fertilizer, wherein the self-made organic fertilizer is prepared from mixing and fermenting Chinese milk vetch, rice bran, tea seed cake, bean dregs, blue algae, marigold, derris, zeolite powder, chicken manure and decay accelerator; field management is carried out until fruit harvesting. The plantation method of the invention can increase the survival rate of navel orange grafting and the quality of products, save planting fertilizer and prevent disease and increase output; the method is suitable for large scale systematic plantation and can increase output and income for farmers.

The invention provides a compound microbial fertilizer special for paddy rice and a preparation method of the fertilizer. The compound microbial fertilizer is made from an organic fertilizer component, an inorganic fertilizer component, a compound microbial agent and a coating material, wherein the coating material is composed of medium chain triglyceride, a solid phase component, and an aqueous phase component. Through effective combination of organic, inorganic and microbial components, the fertilizer is abundant in organic matter components, inorganic nutrients and effective active bacteria. The fertilizer has a good yield-increasing effect-improving function, is good in slow release effect, and has a better anti-disease effect.

The invention relates to a soybean slower-growing rhizobium with broad-spectrum nodulation characteristics, application thereof and a composite rhizobium agent prepared from the same, and belongs to the technical field of agricultural microorganisms. In order to solve the problems that the soybean nodule bacteria are low in nodulation rate and poor in nitrogen fixing effect, the invention providesa Japanese Bradyrhizobium japonicum HF25, the preservation number is CGMCC No.14857, the bacterial strain has high matching affinity, nodulation competitiveness and nodulation and nitrogen fixation capacity. The invention further provides the composite rhizobium agent which is prepared from Japanese Bradyrhizobium japonicum HF25 and Klebsiella oxytoca ASP-15, two kinds of bacteria are compounded,and the nodulation and nitrogen fixation capacity is significantly improved; the composite rhizobium agent is applied to soybean planting, compared with soybeans which are not applied with a microbial agent, the number of soybean nodules is increased by 170%, the number of pods per plant is increased by 37.3%, and the hundred-grain weight of soybeans is increased by 5.5%.

The invention relates to kosakonia radicincitans and an application thereof. The kosakonia radicincitans GXGL-4A is preserved in China General Microbiological Culture Collection Center on June 2, 2016 with registered preservation number of CGMCC No.12588, and the sequence of a 16S rRNA gene part is shown as SEQ ID NO.1. The nitrogen-fixing bacterium GXGL-4A provided by the invention has an excellent microbial nitrogen-fixing capacity; and in a non-nitrogen Ashby medium, a nitrogen-fixing enzyme activity is 232.94+/-8.82nmol of C2H4/(mL.h). In addition, the K.radicincitans GXGL-4A provided by the invention has a good ammonium-secreting capacity; and by cultivating the GXGL-4A on the non-nitrogen Ashby solid medium, the content of the detected extracellular ammonium nitrogen is 2.51 [mu]g/mL. The strain (the kosakonia radicincitans), which has a capsule, can resist bad external environmental conditions such as ultraviolet ray, drought and the like, and the strain has a significant effect of promoting the growth of such crops as corn, rice and the like. The strain can be used for preparing bacterial manure and can reduce the application amount of a nitrogen fertilizer, so as to achieve effects of increasing production, preventing eutrophication of a water body, protecting environment and the like.

The invention discloses an ecological cultivation method for intercropping atractylodes macrocephala koidz and soybeans, and belongs to the technical field of atractylodes macrocephala koidz cultivation. The cultivation method comprises the following steps of soil preparation and soil improvement, seed selection and disinfection, fertilization and bedding, soybean seedling raising, transplanting, field management and orderly harvesting. According to the ecological cultivation method, the atractylodis macrocephala koidz and the soybeans are intercropped, soybean root nodules have high nitrogen fixation capacity, and soil can be continuously fertilized in the growth process. The invention provides a method for infecting the root system of the atractylodes macrocephala koidz through exogenous (soybean, wild soybean and peanut) rhizobium for the first time. According to the method, the atractylodes macrocephala koidz obtains natural nitrogen fixation capacity, the input of nitrogen fertilizer is greatly reduced, and the requirement of the atractylodes macrocephala koidz for the nitrogen fertilizer can be met; in addition, soil conditioner, slow-release biological organic fertilizer and root nodule biological composite liquid are combined, so that soil-borne pathogenic bacteria in soil can be effectively killed, heavy metal in the soil is passivated, the activity of nitrogenase in the soil is improved, the planting cost is effectively reduced, and the purposes of safety, high quality and high yield of the atractylodes macrocephala koidz are achieved.

The invention provides a preparation method of an active ferment concentrated liquid fertilizer. The preparation method of the active ferment concentrated liquid fertilizer belongs to the technical field of preparation methods of liquid fertilizers. The preparation method comprises the following steps of respectively adding water into 17-21kg of artemisia argyi and 49-61kg of soybean, and cooking thoroughly; mixing with 9-13kg of brown sugar, 49-61kg of ammonium sulfate, 24-31kg of zinc sulfate, 24-31kg of monopotassium phosphate, 24-31kg of magnesium sulfate, 24-31kg of boric acid, 14-21kg of ammonium molybdate and 19-26kg of compound enzyme, and adding water until the weight is 1,000kg; fermenting at normal temperature for 15-18 days; filtering a solid matter to obtain the liquid fertilizer. The liquid fertilizer prepared by the invention contains rich active protein, auxin and other matters, also contains boron, molybdenum, zinc, iron, magnesium, calcium and other microelements, has complex functions of strengthening rhizome, increasing flower and fruit bearing, expanding and coloring, improving yield, improving quality and the like, and has very strong immunity and control capacity on various plant diseases and insect pests.

The invention discloses a method for cultivating organic ecological grapes. The method includes the following steps that 1, a place with open terrain, abundant sunshine, good water sources and thick soil is selected as a planting area; 2, a shed suitable for grape growth is constructed; 3, grape seedlings are transplanted in a greenhouse in March and planted in ridges, a row of grape seedlings areplanted in each ridge, and the plant spacing is set as 0.6-1 meters; 4, Chinese trumpet creeper is sowed in soil for grape planting; 5, chickens, ducks and geese are raised in the greenhouse for grape planting; 6, the Chinese trumpet creeper serves as green manure of the grape planting area, has strong nitrogen fixation ability, and can fertilize the soil while providing non-polluting organic nitrogen for the grapes; the excrement of chickens, ducks and geese can also be used as an organic fertilizer to promote the growth of the grapes. The Chinese trumpet creeper is sowed in the greenhouse,chickens, ducks and geese are raised in the greenhouse, the grass control and fertilization effect can be achieved, the use of a chemical fertilizer is reduced, soil is protected, the environment is protected, and the pure natural and pollution-free grapes can be cultivated out.

The invention provides a mesorhizobium kowhaii and Astragalus mongholicus rhizobium agent, a method and an application, and belongs to the technical field of microbial agents. The mesorhizobium kowhaii YS-1 provided by the invention has the preservation number of CGMCC No.20571, and has the characteristics of high nodulation rate and strong nitrogen fixation capability, can significantly improve the summarized nodulation number, effective nodulation number and nodulation weight of astragalus membranaceus, reduces the use amount of chemical nitrogen fertilizer in astragalus membranaceus planting, reduces the pollution of nitrogen fertilizer to the environment, and plays a significant role and has important and profound significance on production of green food and high yield, high efficiency and low consumption of agriculture.

The invention relates to a corn field acid soil improving fertilizer. The corn field acid soil improving fertilizer consists of an inner layer and an outer layer, wherein the inner layer is prepared from the following components in parts by weight: 5 to 15 parts of urease, 10 to 15 parts of cellulase, 10 to 15 parts of lactic acid bacteria agent and 10 to 15 parts of silicate bacterial agent; the outer layer is prepared from the following components in parts by weight: 20 to 30 parts of astragalus sinicus powder, 10 to 15 parts of quick lime, 15 to 25 parts of polyacrylamide, 5 to 15 parts of oyster shell powder, 1 to 10 parts of humic acid, 1 to 15 parts of palm oil, 1 to 5 parts of potassium polyphosphates, 10 to 20 parts of bone powder, 10 to 20 parts of phosphorite powder, 20 to 30 parts of bentonite, and 1 to 5 parts of trace elements. By adopting the corn field acid improving fertilizer, nutrients of soil are increased, and the water retention performance of land is improved.

The invention discloses a nitrogen-fixing bradyrhizobium japonicum strain resistant to saline-alkali soil and application of the bradyrhizobium japonicum strain. According to the invention, a strain of bradyrhizobium taihuae Dian strain is obtained through separation and is preserved in Guangdong Microbial Culture Collection Center on January 11, 2022, and the preservation number is GDMCC No: 62202. Researches show that the strain has the characteristics of high nodulation rate and strong nitrogen fixation capability, and can optimize the micro-ecological environment of alkaline soil crop root growth; the Dian strain is adopted to treat plants, soybean nodulation and nitrogen fixation can be effectively promoted, plant nitrogen nutrition is increased, plant growth is promoted, and the biomass, root nodule number and nitrogen content of soybean plants can be remarkably improved. The strain is especially suitable for cultivation and production of soybeans in stony desertification areas, expands a strain resource library of nitrogen-fixing rhizobium, is beneficial to promoting nodulation and nitrogen fixation of plants, and increases nitrogen nutrition of the plants.

A cultivation method for improving the water-saving and drought-resistant ability of sweet potatoes in spring in the Huang-Huai-Hai area comprises the following steps: selecting a rareripe fresh-eating sweet potato variety with good quality, high commodity property and strong drought resistance and cold resistance; selecting a sandy land block; planting astragalus including seed preprocessing, seed dressing, sowing, field management, crushing and returning; planting sweet potatoes including adjustment of the planting time and the planting density of potato seedlings and the types and the application amount of fertilizers and disease and pest prevention and control, so that the eating quality and the commodity of the sweet potatoes are improved. According to the cultivation method, the adjustment is carried out from the aspects of variety, planting soil selection, soil moisture preservation, fertilization, planting, disease and pest prevention and control and the like, so that the soildryness is greatly reduced, the transplanting survival rate of sweet potato seedlings is remarkably improved, the soil organic matter content is increased, the yield of the sweet potatoes is increased, the appearance commodity of the sweet potatoes is greatly improved, and the size of the cultivated sweet potatoes is moderate and uniform; the cultivated sweet potatoes are bright in skin color, good in taste after cooking and high in commodity property, and the income of farmers is increased; the method has important economic benefits.

The invention belongs to the technical field of medicinal material planting, and particularly relates to an ecological cultivation method for intercropping fritillaria hupehensis and viciafaba, which comprises the following steps: S1, land selection and preparation; S2, seed selection and disinfection; S3, fertilizing and bedding; S4, transplanting; S5, field management; S6, timely harvesting. According to the method, the fritillaria hupehensis and viciafaba are intercropped, on one hand, viciafaba can shade the fritillaria hupehensis after growing tall and growing up, the fritillaria hupehensis can well develop and grow, the biological diversity is improved, and diseases, pests and weeds can be effectively reduced. On the other hand, the complementary effect of crop space and resource utilization can be achieved, the light and heat utilization efficiency of the overground part can be improved, the water and nutrient utilization efficiency of the underground part can be improved, and the planted viciafaba can bring extra economic benefits. Meanwhile, root nodules of viciafaba have high nitrogen fixation capacity, natural nitrogen fertilizer and other nutrients are provided for fritillaria hupehensis, and therefore investment of chemical fertilizer and basic fertilizer is reduced, infectious diseases are reduced, and the labor cost is lowered.