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Compound photosynthetic bacteria preparation for enhancing phytoremediation for heavy metal pollution of soil and preparation method thereof

A photosynthetic bacteria preparation, phytoremediation technology, applied in the direction of restoration of polluted soil, etc., can solve the problems of open technical scheme of the preparation method of S. rosacea, unfavorable large-scale application, slow growth rate, etc. The effect of promoting material flow and promoting growth

Inactive Publication Date: 2015-06-17
北京聚益成广科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But to put it into practical use, the first problem to be solved is that the growth rate of the bacteria is slow, and it usually takes two weeks to cultivate for a clear growth trend. It can be used as a bacterial agent after 20-40 days of cultivation, and the value of OD650 Not higher than 1, the live bacteria content is lower than 100 million / ml, and the long growth cycle is not conducive to large-scale application
And up to the present, also do not see relevant technical scheme of preparation method of Sulfur persicae to disclose
There is also no disclosure of a technical solution for the mixed fermentation of Thiobacillus peach and Rhodopseudomonas capsulata, nor a technical solution for strengthening phytoremediation of soil heavy metals.

Method used

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  • Compound photosynthetic bacteria preparation for enhancing phytoremediation for heavy metal pollution of soil and preparation method thereof
  • Compound photosynthetic bacteria preparation for enhancing phytoremediation for heavy metal pollution of soil and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] The preparation method of the photosynthetic bacteria preparation for strengthening phytoremediation of heavy metal pollution in soil adopts the following steps to cultivate the Sulfur pericum species in the purple sulfur photosynthetic bacteria and the rhodopseudomonas capsula in the purple non-sulfur photosynthetic bacteria:

[0045] 1. Activation and cultivation of the semi-solid seeds of Thiobacillus tarotrichum: puncture the species of Thiobacillus tarotrichum in the semi-solid medium of Thiobacillus tarotrichum, and culture it under light at 30°C for 8 days, and the bacterium thread to be punctured turns red and grows a lawn, that is It may be an activated Sulfur pericum species;

[0046] 1. Plate activation of Rhodopseudomonas capsulata: Streak and activate Rhodopseudomonas capsulata strains on the plate, activate and cultivate at 30°C for 4 days, and pick large colonies as activated seeds;

[0047] 1. Cultivation of Sulfur sulphurum seed: inoculate the acti...

Embodiment 2

[0057] The preparation method of the photosynthetic bacteria preparation for strengthening phytoremediation of heavy metal pollution in soil adopts the following steps to cultivate the Sulfur pericum species in the purple sulfur photosynthetic bacteria and the rhodopseudomonas capsula in the purple non-sulfur photosynthetic bacteria:

[0058] 1. Activation and cultivation of the semi-solid seeds of Thiobacillus tarotrichum: puncture the species of Thiobacillus tarotrichum in the semi-solid medium of Thiobacillus tarotrichum, and culture it under light at 30°C for 8 days, and the bacterium thread to be punctured turns red and grows a lawn, that is It can be an activated Sulfur pericum species strain;

[0059] 1. Plate activation of Rhodopseudomonas capsulata: Streak and activate Rhodopseudomonas capsulata strains on the plate, activate and cultivate at 30°C for 4 days, and pick large colonies as activated seeds;

[0060] . Seed cultivation of Sulfur persicum: inoculate th...

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Abstract

The invention provides a compound photosynthetic bacteria preparation for enhancing the phytoremediation for heavy metal pollution of soil and a preparation method thereof. The high concentration compound photosynthetic bacteria preparation of thiocapsa roseopersicina and rhodopseudomonas capsulata is adopted through the step that thiocapsa roseopersicina cultures in purple sulfur photosynthetic bacteria and rhodopseudomonas capsulate cultures in purple non-sulfur photosynthetic bacteria are subjected to such culture steps as seed activation, seed culture and anaerobic fermentor irradiation culture. The compound photosynthetic bacteria preparation is used for enhancing the phytoremediation for heavy metal pollution of soil, thiocapsa roseopersicina as sulfur-oxidizing bacteria can be used for oxidizing relatively stable sulfides with heavy metals, thereby promoting sulfur cycle of soil, reducing the PH value of soil and improving the activity of heavy metals; the thiocapsa roseopersicina and rhodopseudomonas capsulata as bacterial manure can promote the conversion of soil substances, improve the soil structure and soil fertility, promote plant growth and improve the biomass of plants enriched with heavy metals, thereby enhancing the efficiency of phytoremediation for heavy metals in soil.

Description

technical field [0001] The invention belongs to the technical field of functional microbial preparation and agriculture and environmental pollution control, and specifically relates to a composite photosynthetic bacteria preparation for strengthening phytoremediation of soil heavy metal pollution and a preparation method thereof. Background technique [0002] Because heavy metals are difficult to degrade, migrate and have a long period of harm in soil, soil heavy metal pollution has become one of the environmental problems that are widely concerned at home and abroad. Countries all over the world have attached great importance to heavy metal pollution and its treatment. However, the commonly used chemical and physical chemical remediation methods are generally expensive in investment, complex in equipment, and easily lead to soil structure damage; while phytoextraction remediation technology is usually short, slow in growth, and low in biomass due to hyperaccumulation of plan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
Inventor 蒋常德
Owner 北京聚益成广科技有限公司
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