Preparation method of compound biological fertilizer for soil controlling
A compound biological fertilizer and soil technology, which is applied in the field of compound biological fertilizer preparation, can solve the problems of short time for fertilizer to exert its fertilizer effect, pollutants are not easy to be absorbed, and incomplete nutrition, etc. It can achieve strong ion exchange capacity, prolong the time of fertilizer effect, improve The effect of physical and chemical properties
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Embodiment 1
[0032] Soil Sample: Orchard Soil
[0033] Biochar modified liquid: 10mol / LHN0 3 Solution, 1mol / LHN0 3 Solution, 1mol / L NaOH solution, and distilled water were mixed uniformly at a volume ratio of 10:18:15:100.
[0034] Azotobacter isolation and screening medium (nitrogen-free medium): in parts by mass, take 8 parts of mannitol, KH 2 P0 4 0.1 parts, MgSO 4 ·7H 2 0 0.02 part, NaCl 0.1 part, CaS0 4 2H 2 0 0.06 parts, CaC0 3 3 parts, 1000 parts of water, 15 parts of agar, pH value 7.2±0.2, sterilized at 121°C for 15 minutes.
[0035] Phospholytic bacteria isolation and screening medium: by mass parts, get 12 parts of glucose, (NH 4 ) 2 SO 4 0.3 part, KCl0.1 part, MgS0 4 ·7H 2 0 0.3 parts, Ca 3 (P0 4 ) 2 12 parts, FeS0 4 ·7H 2 0 0.02 parts, MnS0 4 4H 2 0.02 parts, 1000 parts of water, 15 parts of agar, pH value 7.0±0.2, sterilized at 121°C for 15 minutes.
[0036] Potassium-solubilizing bacteria isolation and screening medium: by mass parts, take 12 parts of...
Embodiment 2
[0043] Soil Sample: Orchard Soil
[0044] Biochar modified liquid: 10mol / LHN0 3 Solution, 1mol / LHN0 3 Solution, 1mol / L NaOH solution, and distilled water were mixed uniformly at a volume ratio of 10:18:15:100.
[0045] Azotobacter isolation and screening medium (nitrogen-free medium): by mass parts, take 9 parts of mannitol, KH 2 P0 4 0.2 parts, MgSO 4 ·7H 2 0 0.04 part, NaCl 0.1 part, CaS0 4 2H 2 0 0.08 parts, CaC0 3 4 parts, 1000 parts of water, 17 parts of agar, pH value 7.2±0.2, sterilized at 121°C for 15 minutes.
[0046] Phospholytic bacteria isolation and screening medium: by mass parts, get 13 parts of glucose, (NH 4 ) 2 SO 4 0.4 parts, KCl0.2 parts, MgS0 4 ·7H 2 0 0.4 parts, Ca 3 (P0 4 ) 2 14 parts, FeS0 4 ·7H 2 0 0.06 parts, MnS0 4 4H 2 0.04 parts, 1000 parts of water, 17 parts of agar, pH value 7.0±0.2, sterilized at 121°C for 15 minutes.
[0047] Potassium-solubilizing bacteria isolation and screening medium: by mass parts, take 13 parts of ...
Embodiment 3
[0054] Soil Sample: Orchard Soil
[0055] Biochar modified liquid: 10mol / LHN0 3 Solution, 1mol / LHN0 3 Solution, 1mol / L NaOH solution, and distilled water were mixed uniformly at a volume ratio of 10:18:15:100.
[0056] Azotobacter isolation and screening medium (nitrogen-free medium): in parts by mass, take 10 parts of mannitol, KH 2 P0 4 0.3 parts, MgSO 4 ·7H 2 0 0.05 part, NaCl 0.2 part, CaS0 4 2H 2 0 0.1 parts, CaC0 3 5 parts, 1000 parts of water, 20 parts of agar, pH value 7.2±0.2, sterilized at 121°C for 15 minutes.
[0057] Phospholytic bacteria isolation and screening medium: by mass parts, get 15 parts of glucose, (NH 4 ) 2 SO 4 0.5 parts, KCl0.3 parts, MgS0 4 ·7H 2 0 0.5 parts, Ca 3 (P0 4 ) 2 15 parts, FeS0 4 ·7H 2 0 0.07 parts, MnS0 4 4H 2 0.08 parts, 1000 parts water, 20 parts agar, pH value 7.0±0.2, sterilized at 121°C for 15 minutes.
[0058] Potassium-solubilizing bacteria isolation and screening medium: by mass parts, take 15 parts of sucr...
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