A strain of Bacillus tequila s1 with nitrogen-fixing function and its application in composting

A technology of Bacillus tequila and Bacillus, applied in the field of microorganisms, can solve the problems of inhibition of microbial population activity, actual utilization rate of less than 40%, decrease of population abundance, etc. The effect of strong nitrogen fixation ability

Active Publication Date: 2022-03-18
湖北都兴隆农业技术有限公司
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Problems solved by technology

[0002] my country is rich in organic waste resources, but the actual utilization rate is less than 40%
However, the degradation and conversion of cellulose, the main carbon source component in the raw material, mainly occurs in the high temperature stage of composting, but the high temperature inhibits the activity of microbial populations, which is manifested in the decrease of the abundance of microbial populations in the high temperature stage and the The reduction in the number of viable bacteria is generally 1 to 2 orders of magnitude lower than that in the heating stage of composting
More importantly, during the composting process, microorganisms first decompose other nitrogenous substances in organic waste into ammonia nitrogen, which are then utilized or transformed by microorganisms, and the ammonia nitrogen produced by decomposition and / or the ammonia nitrogen contained in the raw materials themselves are used in composting. The high temperature stage is easy to be volatilized by heat, which leads to insufficient nitrogen source required by microorganisms in the high temperature stage of cellulose degradation stage, and limits the degradation and transformation of cellulose by microorganisms to a certain extent.

Method used

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  • A strain of Bacillus tequila s1 with nitrogen-fixing function and its application in composting
  • A strain of Bacillus tequila s1 with nitrogen-fixing function and its application in composting
  • A strain of Bacillus tequila s1 with nitrogen-fixing function and its application in composting

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: Isolation, screening and identification of bacterial strain S1

[0028] Isolation of strains: from the experimental base of Hubei Tiantou Biotechnology Co., Ltd. (Yaqueling "Vineyard" demonstration base) in Group 5 of Shuifumiao Village, Longquan Town, Yiling District, Yichang City, the surface layer of farmland with good ecology (2-5cm deep ) 5 cultivated soil samples. Equal amounts of soil samples were uniformly mixed in the laboratory, and 10 g of mixed soil samples were put into a conical flask filled with 90 mL of sterile water, shaken at 150 rpm at 37°C for 30 min to make a suspension. Take 10mL of the suspension, add it to a sterile test tube, and heat it in a boiling water bath for 10 minutes, then prepare the suspension with sterile water to a concentration of 10 -7 Take the dilution of 0.1mL of the dilution and spread it on the LB solid medium. Place the coated plate at 60°C and culture it. If it can grow and form a single colony within 3 days,...

Embodiment 2

[0034] Embodiment 2: the production of bacterial strain S1 fermented liquid

[0035] The bacterial strain Bacillus tequila strain S1 (CCTCC NO: M2020487) of Example 1 was inoculated into the glucose corn steep liquor dry powder liquid medium for fermentation and expansion. The fermentation condition of the 50L fermenter is pH 6.0-7.0, the fermentation temperature is 37°C, the stirring speed is 150-250rpm, the fermentation time is 36h, and the concentration of bacteria in the fermentation liquid is OD at the end of the fermentation 600 =12~15. The preparation method of the glucose corn steep liquor powder liquid medium used is: glucose 1.5g / L, corn steep liquor dry powder 4g / L, adjust the initial pH value to 7.5, sterilize at 115°C for 30min, and the volume of the fermentation medium is 30L. If feeding is required during the fermentation process, the preparation method of the feeding medium is as follows: glucose 15g / L, corn steep liquor powder 40g / L, adjust the initial pH val...

Embodiment 3

[0036] Embodiment 3: composting experiment

[0037] The yeast waste liquid concentrate (provided by Angel Yeast Co., Ltd., specifically the fermentation waste liquid produced by yeast production) was obtained after evaporation and concentration. Its properties were brown viscous liquid. 35.6%, total nitrogen 2.6%, ammoniacal nitrogen 0.8%, P 2 o 5 0.5%, K 2 (6.5%, pH 5.6) and rice husk are initially mixed according to mass ratio 0.45:0.55, until the flow of yeast waste liquid concentrate is not observed, insert the bacterial strain Bacillus tequila strain S1 of embodiment 2 according to 0.5% of the total mass of compost raw materials The fermented liquid is replenished with water until the moisture content of the heap is 50% to 55%. After mixing evenly, the heap grows into a nearly cubic heap (abbreviation: experimental group, the same below) with a length of 10 meters, a width of 4 meters, and a height of 1.5 meters. Use a forklift to turn the compost during the compostin...

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Abstract

The invention discloses a strain of Tequila bacillus Bacillus tequila strain S1, which is isolated from ecologically good cultivated soil, has strong nitrogen fixation ability, and has certain high temperature resistance and cellulose degradation ability. The invention also discloses the application of the bacillus tequila S1 in the mixed compost using the yeast waste liquid concentrate and rice husk as raw materials. Carry out liquid fermentation of Bacillus tequila S1, and inoculate the fermentation broth into the above-mentioned compost raw materials. When other nitrogen sources are reduced during the high temperature period of composting, Bacillus tequila S1 can absorb nitrogen in the air as a nitrogen source to make up for nitrogen Insufficient sources and promote the degradation of cellulose in compost raw materials, thereby effectively shortening the composting cycle, and increasing the humic acid and total nitrogen content of compost products, and ultimately achieve the purpose of improving compost efficiency and compost product fertilizer efficiency.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain of Bacillus tequila S1 with nitrogen-fixing function and its application in mixed compost using concentrated yeast waste liquid and rice husk as raw materials. Background technique [0002] my country is rich in organic waste resources, but the actual utilization rate is less than 40%. The composting of organic fertilizer waste to produce organic fertilizer has the advantages of economy and simplicity. It not only deals with pollution, but also realizes the resource utilization, fertilizer transformation, return to soil and reduction of chemical fertilizer consumption of organic waste, which is beneficial to the sustainable development of economy and ecology. Significance. Straw, fruit and vegetable residues, poultry and livestock manure, and kitchen waste are the main sources of organic waste for composting to produce organic fertilizers. At the same time, the compos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F17/20C12R1/07
CPCC12N1/20C05F17/20Y02W30/40
Inventor 任立伟孙丹丹张明星古明慧汪云飞郭金玲吕育财龚大春
Owner 湖北都兴隆农业技术有限公司
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