Mesorhizobium kowhaii and culture method thereof, astragalus membranaceus rhizobium agent and method and application of astragalus membranaceus rhizobium agent

A technology of intermediate rhizobia and astragalus root nodules, applied in application, horticultural methods, microbial-based methods, etc., can solve the problems of reduced efficiency of symbiotic relationship, low efficiency of nitrogen fixation, etc.

Active Publication Date: 2021-06-22
INST OF SOIL FERTILIZER & WATER SAVING AGRI GANSU ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, soils often harbor some indigenous rhizobia with low or ineffec

Method used

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  • Mesorhizobium kowhaii and culture method thereof, astragalus membranaceus rhizobium agent and method and application of astragalus membranaceus rhizobium agent
  • Mesorhizobium kowhaii and culture method thereof, astragalus membranaceus rhizobium agent and method and application of astragalus membranaceus rhizobium agent
  • Mesorhizobium kowhaii and culture method thereof, astragalus membranaceus rhizobium agent and method and application of astragalus membranaceus rhizobium agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Isolation and Identification of Interrhizobia YS-1 from Sophora japonica

[0030] Sophora japonica interrhizobia YS-1 of the present invention is isolated and differentiated from a sample of wild astragalus root nodules located in Banyinpo Village, Huichuan Town, Weiyuan, Gansu Province, and is preserved in the General Microbiological Cultures Preservation Center of China Microbial Cultures Preservation Management Committee. No. CGMCC No.20571. The characteristics of the colonies of the present invention are round, colorless, smooth, convex, with a moist surface, and the colonies are colorless, see for details figure 1 ; The cell morphology is shown in Table 1.

[0031] Table 1 Morphology of Sophora japonica interrhizobia YS-1

[0032]

[0033] Note: "+" is positive; "-" is negative

[0034] Physiological and biochemical characteristics: The utilization of different carbon sources and nitrogen sources and the determination of chemical sensitive substances of YS-1 we...

Embodiment 2

[0045] Cultivation method of interrhizobia YS-1 in Sophora japonica

[0046] Sophora japonica intermedium rhizobium YS-1 was inoculated into the fermentation medium for cultivation; wherein, the fermentation medium was composed of the following concentrations of components: mannitol 10g / L, potassium dihydrogen phosphate 0.25g / L, dipotassium hydrogen phosphate 0.25g / L, magnesium sulfate 0.2g / L, sodium chloride 0.1g / L and calcium carbonate 0.3g / L, pH 7.0; at a temperature of 27°C, a stirring speed of 200rpm, and an air flow of 4.8m 3 Cultivate under the condition of 4 days per hour, after the end of the cultivation, the number of viable bacteria of Sophora japonica interrhizobia YS-1 was 1×10 9 CFU / mL.

Embodiment 3

[0048] An astragalus rhizobia agent, which contains Sophora japonica interrhizobia YS-1 of the present invention, wherein the number of viable bacteria of Sophora japonica interrhizobia YS-1 is 1×10 9 CFU / mL. The astragalus rhizobium bacterial agent of the present invention is obtained by fermenting the culture method of Example 2.

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Abstract

The invention provides a mesorhizobium kowhaii and Astragalus mongholicus rhizobium agent, a method and an application, and belongs to the technical field of microbial agents. The mesorhizobium kowhaii YS-1 provided by the invention has the preservation number of CGMCC No.20571, and has the characteristics of high nodulation rate and strong nitrogen fixation capability, can significantly improve the summarized nodulation number, effective nodulation number and nodulation weight of astragalus membranaceus, reduces the use amount of chemical nitrogen fertilizer in astragalus membranaceus planting, reduces the pollution of nitrogen fertilizer to the environment, and plays a significant role and has important and profound significance on production of green food and high yield, high efficiency and low consumption of agriculture.

Description

technical field [0001] The invention belongs to the technical field of microbial bacterial agents, and in particular relates to a Sophora japonica interrhizobia strain and a cultivation method thereof, an astragalus root nodule agent, a method and an application thereof. Background technique [0002] The effectiveness of agricultural microorganisms runs through the entire process of agricultural production. Strengthening the research and industrial application of agricultural microbial resources has a positive role in promoting the transformation of agricultural industrial structure and increasing farmers' income. The use of microbial fertilizers or bacterial agents with environmental protection functions has become an important part of agricultural development. the only way. [0003] Astragalus is a perennial herb of the leguminous family. Its roots are used as medicine. It is warm in nature and sweet in taste. It can invigorate qi and strengthen the surface. The main acti...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01G7/06A01G22/40C12R1/41
CPCC12N1/20A01G7/06A01G22/40
Inventor 王文丽李娟
Owner INST OF SOIL FERTILIZER & WATER SAVING AGRI GANSU ACAD OF AGRI SCI
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