Mesorhizobium KDRM319 and application thereof
A brachyrhizobium and gene technology, applied in the field of brachyrhizobia KDRM319, can solve problems such as complex mechanisms, and achieve the effects of increasing biomass, promoting growth, and reducing inhibitory effects.
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[0023] 1. Isolation, Purification and Preservation of Rhizobia
[0024] Select the large and full root nodules on the roots of robust black locust from the wild black locust in Guishan, Danjiangkou City, Hubei Province, carefully cut off the root nodules and some roots with scissors, and put 5-15 root nodules obtained from the same root of black locust into the dry discoloration silica gel And in a small tube covered with absorbent cotton. Then the collected nodules were fully soaked in sterile water for imbibition, soaked in 95% alcohol for 30 s, then sterilized with 0.1% mercury chloride for 5 min, and rinsed with sterile water for 6 times. Put them into a sterile 2-ml centrifuge tube after numbering, grind the root nodules with a sterile grinding rod, add 1 ml of sterile water with a pipette and suck and drain the homogenate 3 times to suspend the homogenate, and serially dilute the suspension 10 times, 100 times and 1000 times, and then pipette 100 μl of the suspension to...
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