A kind of broad bean rhizobium strain bdz5-3 and its application

Abstract

A broad bean rhizobium strain Bdz5‑3 and application thereof, the bacterial strain is isolated and purified from fresh broad bean root nodules, and belongs to Rhizobium anhuiense strain. The strain was deposited in the Chinese Type Culture Collection Center of Wuhan University on October 23, 2017, and the preservation number is: CCTCC NO: M 2017612. The rhizobia strain Bdz5‑3 of the present invention is an excellent broad-spectrum faba bean rhizobia with strong symbiotic nitrogen fixation ability, wide adaptability to Sichuan broad bean varieties, secretion of IAA, ability to dissolve inorganic phosphorus and organic phosphorus, and strong stress resistance The strain has good matching affinity with the main broad bean varieties in Sichuan. In the production of different varieties of broad beans in Sichuan, no nitrogen fertilizer and inoculation with Bdz5‑3 can increase the yield of broad beans by more than 33.2%, and the difference from the control without inoculation reaches a significant level.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a broad bean rhizobium strain Bdz5-3 and its application. Background technique [0002] China is the largest broad bean producer, with dry broad bean production accounting for 35% of the world (FAO2014). It is an important agricultural product resource for my country's traditional foreign trade, and its export volume ranks second among the country's miscellaneous grains (Liu Yujiao et al., 2002). The sown area of ​​broad beans in Sichuan Province is about 300,000 hectares, second only to Yunnan, with a total output of 600 million kilograms (Yang Wuyun, 2003). Douban in Pixian County, Sichuan, which uses broad beans as raw materials, is well-known throughout the country and is in great demand. The annual demand for broad beans reaches 120,000 tons, but now it mainly relies on imports from other places. In addition to harvesting dry broad beans, fresh broad beans can also be harveste...

AI Technical Summary

Problems solved by technology

Shi Shangli (2007) conducted research on more than 730 strains of alfalfa rhizobia, and only 10 strains of rhizobia with strong ability to secrete IAA were screened from these 29 strains, and the phosphorus-dissolving ability of these 29 strains was measured, and it was found that all The strains did not have the ability to dissolve inorganic phosphorus. The 29 Rhizobium meliloti can dissolve organic phosphorus, but the phosphorus-dissolving ability is quite different. Only 8 strains have strong ability to dissolve organic phosphorus, and the rest have weak or weak ability to dissolve organic phosphorus. Organophosphate capacity

[0006] At present, there are no relevant reports on the screening application of high-efficiency rhizobia in broad bean except Qinghai, Gansu (Diao Zhimin, 2002; Han Mei et al., 2010; Wang Wenli et al., 2010) and Hebei (Wang Rui, 2016). other reports

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