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Compound microbial agent suitable for saline-alkali soil in arid and semi-arid regions and application of compound microbial agent

A compound microbial inoculant and semi-arid technology, applied in the field of microorganisms, can solve problems such as threats to the ecological environment and food security, high requirements for water sources and irrigation conditions, and few types of special fertilizers for saline-alkali improvement. Dry, easily absorbed effect

Active Publication Date: 2022-04-12
领先生物农业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]Currently, the main means of salinity improvement of cultivated land is to wash and leaching the land to reduce the salinity content of the soil, and then plant salt-alkali-tolerant crops. However, this method It has high requirements on water source and irrigation conditions, and the scope of application is limited. The water resources in Shaanxi, Gansu, Ningxia and other regions are difficult to meet the needs of salinity improvement.
Therefore, salinity-alkali improvement in areas lacking in water resources should be based on soil conditioning, and fertilizers should be used together with the planting of alkali-resistant plants; It is imminent to find a suitable and ecologically beneficial improvement method for my country's ecological environment and food security

Method used

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  • Compound microbial agent suitable for saline-alkali soil in arid and semi-arid regions and application of compound microbial agent
  • Compound microbial agent suitable for saline-alkali soil in arid and semi-arid regions and application of compound microbial agent
  • Compound microbial agent suitable for saline-alkali soil in arid and semi-arid regions and application of compound microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Preparation of functional water-retaining peptides of different molecular weights

[0037] Referring to the disclosed method of CN104694437A, the functional water-retaining peptide γ-PGA of different molecular weights is prepared by fermentation of Bacillus licheniformis CGMCC No.8821, and the functional water-retaining peptide γ-PGA of different molecular weights is obtained by changing the fermentation carbon source; wherein, glucose is used to prepare the molecular weight of 3.0×10 5 Da's functional water-retaining peptide γ-PGA, prepared from glycerol with a molecular weight of 5.0×10 5 Da's functional water retention peptide γ-PGA prepared from molasses with a molecular weight of 7.0×10 5 Da's functional water retention peptide γ-PGA.

[0038] The water absorption multiples of the above three functional water-retaining peptides γ-PGA with different molecular weights are determined, and the method is as follows:

[0039] Weigh the functional water-retai...

Embodiment 2

[0042] Drought-resistant and growth-promoting effect of embodiment 2 functional water-retaining peptides

[0043] The functional water-retaining peptide (molecular weight 5.0×10 5 Da) carry out the anti-drought stress test on Chinese cabbage.

[0044] The test treatments are respectively: A treatment is a blank control, B treatment is a commercially available water-retaining agent, and C treatment is the functional water-retaining peptide of Example 1 (molecular weight 5.0×10 5 Da), the application rate of processing B and processing C is 100g / mu.

[0045] The test method is as follows: first mix a small amount of soil with commercially available water-retaining agents and functional water-retaining peptides, and spread them evenly on the soil surface, then sow pak choi. Irrigate according to the watering amount of 50% of the field water capacity. After the pakchoi emerge and grow to 2 true leaves, each treatment is subjected to drought treatment, and each treatment is water...

Embodiment 3

[0051] Embodiment 3 anti-drought, saline-alkali tolerance alfalfa rhizobia screening

[0052] The commercial purchase obtains 5 strains of Rhizobium alfalfa, numbered successively as No. 1 to No. 5, and the 5 strains of Rhizobium Medicago are screened for drought resistance and salt-alkali tolerance; the method is as follows:

[0053] 5 strains of Rhizobium meliloti were respectively activated to prepare seed solutions of 5 strains of Rhizobium meliloti, and they were inoculated to different pH conditions (pH7.5, pH8.5, pH9.5) and NaCl content (1% , 3%, 5%), PEG6000 (10%, 15%, 20%) in the YMB liquid medium, cultured at 28 ℃ for 3-5 days, and measured its optical density value under different drought, salinity, pH levels (OD600).

[0054] The measurement results are shown in Table 2.

[0055] Table 2 Drought tolerance, saline-alkali test of Rhizobium meliloti

[0056]

[0057] the result shows:

[0058] Among the 5 strains of alfalfa rhizobia, No. 4 rhizobia had the smal...

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Abstract

The invention provides a compound microbial agent suitable for saline-alkali soil in arid and semi-arid regions and application of the compound microbial agent. The compound microbial agent disclosed by the invention comprises alfalfa rhizobium, functional water-retaining peptide and drought-enduring bacteria, wherein the drought-tolerant bacteria are endophytic bacillus flexus CGMCC (China General Microbiological Culture Collection Center) No.21705, and the functional water retention peptide is prepared by fermenting bacillus licheniformis CGMCC No.8821. The compound microbial agent disclosed by the invention can be used for improving the activity of crop root systems and the drought resistance and saline-alkali resistance of crops, is particularly suitable for crop planting in arid and semi-arid climate regions, obviously improves the nitrogen fixation performance of crops under arid conditions, obviously improves the adaptive capacity of crop root systems under water drought and saline-alkali stress, improves the stress resistance of crops, and improves the yield of crops. The purposes of improving the soil performance and increasing the yield are achieved, and the method has important significance on improvement of the ecological planting environment of saline-alkali soil in arid and semi-arid regions and sustainable development of modern agriculture.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a composite microbial bacterial agent suitable for saline-alkali soil in arid and semi-arid areas and its application. Background technique [0002] Saline-alkali land is a type of land rich in salt. The main reason for the formation of saline-alkali land is the accumulation of carbonate in the soil, so the soil has a high degree of alkalinity. In severe saline-alkali land, plants can hardly survive. The area of ​​saline-alkali land in my country is about 99.13 million hectares, mainly concentrated in arid and semi-arid areas. The development of saline-alkali land can not only expand my country's per capita arable land area, increase food production, but also improve the ecological environment, increase the green area, and achieve sustainable development. Therefore, the improvement and prevention of saline-alkali land is an important research content to promote social and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/22A01N63/20A01N37/46A01N25/00A01P21/00A01C1/08
CPCY02A40/10
Inventor 李丽艳徐延平孙杉杉李婧葛振宇徐志文
Owner 领先生物农业股份有限公司
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