Mesorhizobium KDRM 565 and application thereof

A technology of bradyrhizobium and nucleotide sequence, applied in the field of mesorhizobium KDRM565, can solve problems such as complex mechanism, achieve the effects of increasing biomass, promoting growth, increasing nodulation rate and symbiotic nitrogen fixation

Active Publication Date: 2014-06-25
ZHONGYING CHANGJIANG INTERNATIONAL NEW ENERGY INVESTMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mechanism of rhizobia regulating ACC deaminase is complex

Method used

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  • Mesorhizobium KDRM 565 and application thereof
  • Mesorhizobium KDRM 565 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] 1. Isolation, Purification and Preservation of Rhizobia

[0023] Select the large and full root nodules on the roots of robust black locust from the artificially planted black locust in Anshan, Jiangxia District, Wuhan City, Hubei Province, carefully cut off the root nodules and some roots with scissors, and put 5-15 root nodules obtained from the same root of black locust into the container. Comes in a vial of dry, color-changing silica gel covered with absorbent cotton. Then the collected nodules were fully soaked in sterile water for imbibition, soaked in 95% alcohol for 30 s, then sterilized with 0.1% mercury chloride for 5 min, and rinsed with sterile water for 6 times. Put them into a sterile 2-ml centrifuge tube after numbering, grind the root nodules with a sterile grinding rod, add 1 ml of sterile water with a pipette and suck and drain the homogenate 3 times to suspend the homogenate, and serially dilute the suspension 10 times, 100 times and 1000 times, and ...

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Abstract

The invention relates to Mesorhizobium KDRM 565 and the application of the Mesorhizobium KDRM 565, wherein the Mesorhizobium KDRM 565 is separated from Robinia pseudoacacia root nodule, contains ACC deaminase and can be used for effective carrying out nodulation and promote growth of Robinia pseudoacacia. The Mesorhizobium KDRM 565 strain is preserved in China Center for Type CultureCollection with the preservation number of CCTCC NO:M2012334. The Mesorhizobium KDRM 565 disclosed by the invention contains ACC deaminase which can decompose ACC into alpha-ketobutyrate and NH3, reduce the level of synthesizing ethylene with plant cells, reduce an inhibition function of ethylene on rhizobium infection, improve the nodulation rate of rhizobium and Robinia pseudoacacia and the level of symbiotic nitrogen fixation, provide high-level nitrogen for the growth of the Robinia pseudoacacia on non-fertilized barren wasteland, promote the growth of seedlings of Robinia pseudoacacia and increase the biomass of mature Robinia pseudoacacia, thereby realizing the high yield the matureRobinia pseudoacacia by using inoculation with low cost, and playing a role in Robinia pseudoacacia culture and forestation.

Description

technical field [0001] The invention belongs to the field of applied microbiology, and in particular relates to a bradyrhizobium KDRM565 which is isolated from root nodules of Robinia pseudoacacia, has ACC deaminase, can form nodules efficiently and promotes the growth of Robinia pseudoacacia, and an application thereof. The strain has been preserved in China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 2012334, and the strain name is Bradyrhizobium KDRM565. Background technique [0002] Some prokaryotic microorganisms in nature can synthesize nitrogenase, which can convert N in the air to 2 reduced to NH 3 . The nitrogen fixed by nitrogen-fixing microorganisms accounts for about 65% - 70% of the combined nitrogen on the surface. Among them, the symbiotic system of rhizobia and leguminous plants has the strongest nitrogen fixation ability, accounting for more than 65% of the biological nitrogen fixation. [0003] It is a classic exper...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P21/00C12R1/01
CPCY02P60/21
Inventor 李万里李江川张继泰刘震东牛涛
Owner ZHONGYING CHANGJIANG INTERNATIONAL NEW ENERGY INVESTMENT CO LTD
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