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Mesorhizobium KDRM495 and application thereof

A technology of KDRM495 and bradyrhizobium, applied in the field of applied microbiology, can solve problems such as complex mechanisms, achieve the effect of increasing biomass, increasing nodulation rate and symbiotic nitrogen fixation, and promoting growth

Active Publication Date: 2013-03-20
ZHONGYING CHANGJIANG INTERNATIONAL NEW ENERGY INVESTMENT CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mechanism of rhizobia regulating ACC deaminase is complex

Method used

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  • Mesorhizobium KDRM495 and application thereof
  • Mesorhizobium KDRM495 and application thereof

Examples

Experimental program
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Embodiment Construction

[0023] 1. Isolation, Purification and Preservation of Rhizobia

[0024] Select the large and full root nodules on the roots of Robinia pseudoacacia artificially planted in Yunxi County, Hubei Province, carefully cut off the root nodules and some roots with scissors, and put 5-15 nodules obtained from the same root of Robinia pseudoacacia into the dry color-changing silica gel and in a small tube covered with absorbent cotton. Then the collected nodules were fully soaked in sterile water for imbibition, soaked in 95% alcohol for 30 s, then sterilized with 0.1% mercury chloride for 5 min, and rinsed with sterile water for 6 times. Put them into a sterile 2-ml centrifuge tube after numbering, grind the root nodules with a sterile grinding rod, add 1 ml of sterile water with a pipette and suck and drain the homogenate 3 times to suspend the homogenate, and serially dilute the suspension 10 times, 100 times and 1000 times, and then pipette 100 μl of the suspension to spread on YMA...

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Abstract

The invention relates to mesorhizobium KDRM495 and application thereof. The mesorhizobium KDRM495 is separated from acacia root nodule, contains ACC deaminase, and can efficiently nodulate and promotes acacia growth. A mesorhizobium KDRM495 strain is collected in CCTCC (China Center for Type Culture Collection), and the collection number is CCTCC No: M2012333. The mesorhizobium KDRM495 disclosed by the invention contains the ACC deaminase which can decompose ACC into alpha-ketobutyrate and NH3, lowers the level of synthesizing ethylene from plant cells, reduces the inhibiting effect of ethylene on rhizobium infestation, enhances the nodulation rate and symbiotic nitrogen fixation level of rhizobia and acacia, provides the high-level nitrogen for the growth of the acacia on unfertilized sterile wastelands, promotes the growth of acacia seedlings and increases the biomass of acacia lumbers, thereby achieving the high yield of the acacia lumbers through low-cost inoculation investment and playing a role in the aspect of acacia seedling culture and forestation.

Description

technical field [0001] The invention belongs to the field of applied microbiology, and in particular relates to a bradyrhizobium KDRM495 isolated from root nodules of Robinia pseudoacacia, having ACC deaminase capable of forming nodules efficiently and promoting the growth of Robinia pseudoacacia, and an application thereof. The strain has been preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 2012333, and the strain name is Bradyrhizobium KDRM495. Background technique [0002] Some prokaryotic microorganisms in nature can synthesize nitrogenase, which can convert N in the air to 2 reduced to NH 3 . The nitrogen fixed by nitrogen-fixing microorganisms accounts for about 65% - 70% of the combined nitrogen on the surface. Among them, the symbiotic system of rhizobia and leguminous plants has the strongest nitrogen fixation ability, accounting for more than 65% of the biological nitrogen fixation. [0003] It is a class...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P21/00C12R1/01
Inventor 李万里李江川张继泰陈卫文
Owner ZHONGYING CHANGJIANG INTERNATIONAL NEW ENERGY INVESTMENT CO LTD
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