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Mesorhizobium KDRM611 and application thereof

A KDRM611, a technology of Brachyrhizobia, applied in the field of applied microbiology, can solve problems such as complex mechanisms, and achieve the effect of increasing biomass, improving nodulation rate and symbiotic nitrogen fixation, and reducing inhibitory effects.

Active Publication Date: 2013-04-03
ZHONGYING CHANGJIANG INTERNATIONAL NEW ENERGY INVESTMENT CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mechanism of rhizobia regulating ACC deaminase is complex

Method used

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  • Mesorhizobium KDRM611 and application thereof
  • Mesorhizobium KDRM611 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] 1. Isolation, Purification and Preservation of Rhizobia

[0023] Select the large and full root nodules on the roots of robust black locust from the artificially planted black locust in Anshan, Jiangxia District, Wuhan City, Hubei Province, carefully cut off the root nodules and some roots with scissors, and put 5-15 root nodules obtained from the same root of black locust into the container. Comes in a vial of dry, color-changing silica gel covered with absorbent cotton. Then the collected nodules were fully soaked in sterile water for imbibition, soaked in 95% alcohol for 30 s, then sterilized with 0.1% mercury chloride for 5 min, and rinsed with sterile water for 6 times. Put them into a sterile 2-ml centrifuge tube after numbering, grind the root nodules with a sterile grinding rod, add 1 ml of sterile water with a pipette and suck and drain the homogenate 3 times to suspend the homogenate, and serially dilute the suspension 10 times, 100 times and 1000 times, and ...

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Abstract

The invention relates to Mesorhizobium KDRM611 and the application of the Mesorhizobium KDRM 565. The Mesorhizobium KDRM611 is separated from Robinia pseudoacacia root nodule, contains ACC deaminase and can effective carry out nodulation and promote the growth of the Robinia pseudoacacia. The Mesorhizobium KDRM 611 strain is preserved in China Center for Type CultureCollection with the preservation number of CCTCC NO:M2012335. The Mesorhizobium KDRM 611 disclosed by the invention contains the ACC deaminase which can decompose ACC into alpha-ketobutyrate and NH3, reduce the level of synthesizing ethylene with plant cells, reduce an inhibition function of ethylene on rhizobium infection, improve the nodulation rate of rhizobium and the Robinia pseudoacacia and the level of symbiotic nitrogen fixation, provide high-level nitrogen for the growth of the Robinia pseudoacacia on non-fertilized barren wasteland, promote the growth of seedlings of the Robinia pseudoacacia and increase the biomass of mature the Robinia pseudoacacia, thereby realizing high yield of the mature Robinia pseudoacacia by using inoculation with low cost, and playing a role in Robinia pseudoacacia culture and forestation.

Description

technical field [0001] The invention belongs to the field of applied microbiology, and in particular relates to a bradyrhizobium KDRM611 isolated from root nodules of Robinia pseudoacacia, having ACC deaminase capable of forming nodules efficiently and promoting the growth of Robinia pseudoacacia, and an application thereof. The strain has been preserved in China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 2012335, and the strain name is Bradyrhizobium KDRM611. Background technique [0002] Some prokaryotic microorganisms in nature can synthesize nitrogenase, which can convert N in the air to 2 reduced to NH 3 . The nitrogen fixed by nitrogen-fixing microorganisms accounts for about 65% - 70% of the combined nitrogen on the surface. Among them, the symbiotic system of rhizobia and leguminous plants has the strongest nitrogen fixation ability, accounting for more than 65% of the biological nitrogen fixation. [0003] It is a classic e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G1/00C12R1/41
Inventor 李万里李江川张继泰柳丽杰
Owner ZHONGYING CHANGJIANG INTERNATIONAL NEW ENERGY INVESTMENT CO LTD
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