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A kind of rhizobia and its application

A rhizobia, bacterial agent technology, applied in bacteria, microorganisms, microorganism-based methods, etc., can solve problems such as low survival rate

Active Publication Date: 2018-10-12
领先生物农业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Over the years, my country's forestry department has invested a lot of manpower, material resources, and financial resources in the study of "Dalbergia japonica" seedlings. Although it has been successful, the survival rate is low. By using rhizobia agents, it can form symbiosis with Dalbergia japonica The nitrogen fixation system can improve the survival rate of Dalbergia japonica and promote the growth of plants, which is of great significance to the artificial cultivation of Dalbergia japonica in the future

Method used

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  • A kind of rhizobia and its application
  • A kind of rhizobia and its application
  • A kind of rhizobia and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Embodiment 1: preparation of rhizobia LX-JX01 bacterial agent

[0094] The implementation steps of this embodiment are as follows:

[0095] A. Preparation of liquid DM medium

[0096] Dissolve 1 gram of yeast extract, 10 grams of sucrose, 2 grams of potassium nitrate, 0.4 grams of dipotassium hydrogen phosphate, 0.4 grams of potassium dihydrogen phosphate and 0.15 grams of sodium chloride in distilled water, and dilute to 1000ml with distilled water; then use 1.0N Sulfuric acid or 1.0N sodium hydroxide adjusts the pH value of its solution to 6.8 to obtain the described liquid DM medium;

[0097] B. Preparation of solid DM medium

[0098] According to 18 grams of agar per liter of liquid DM medium, add agar to the liquid DM medium prepared in step A, heat to 100°C to dissolve the agar, and then cool at 40°C to make the DM medium solid state, so the solid DM medium is obtained;

[0099] C. Strain activation

[0100] Transfer the strain CGMCC No.11265 stored on the sl...

Embodiment 2

[0104] Embodiment 2: preparation of rhizobia LX-JX01 bacterial agent

[0105] The implementation steps of this embodiment are as follows:

[0106] A. Preparation of liquid DM medium

[0107] Dissolve 3 grams of yeast extract, 8 grams of sucrose, 1 gram of potassium nitrate, 0.6 grams of dipotassium hydrogen phosphate, 0.5 grams of potassium dihydrogen phosphate and 0.1 gram of sodium chloride in distilled water, and dilute to 1000ml with distilled water; then use 0.1N Sulfuric acid or 0.1N sodium hydroxide adjusts the pH value of its solution to 7.0 to obtain the described liquid DM medium;

[0108] B. Preparation of solid DM medium

[0109] According to 20 grams of agar per liter of liquid DM medium, add agar to the liquid DM medium prepared in step A, heat to 100°C to dissolve the agar, and then cool at 40°C to make the DM medium solid state, so the solid DM medium is obtained;

[0110] C. Strain activation

[0111] Transfer the strain CGMCC No.11265 stored on the slant...

Embodiment 3

[0115] Embodiment 3: preparation of rhizobia LX-JX01 bacterial agent

[0116] The implementation steps of this embodiment are as follows:

[0117] A. Preparation of liquid DM medium

[0118] Dissolve 2 grams of yeast extract, 12 grams of sucrose, 3 grams of potassium nitrate, 0.5 grams of dipotassium hydrogen phosphate, 0.6 grams of potassium dihydrogen phosphate and 0.2 grams of sodium chloride in distilled water, and dilute to 1000ml with distilled water; then use 2.0N Sulfuric acid or 2.0N sodium hydroxide adjusts the pH value of its solution to 7.2 to obtain the described liquid DM medium;

[0119] B. Preparation of solid DM medium

[0120] According to 19 grams of agar per liter of liquid DM medium, add agar to the liquid DM medium prepared in step A, heat to 100°C to dissolve the agar, and then cool at 40°C to make the DM medium solid state, so the solid DM medium is obtained;

[0121] C. Strain activation

[0122] Transfer the strain CGMCC No.11265 stored on the sl...

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Abstract

The invention relates to Bradyrhizobium sp. LX‑JX01 and its application. The preparation method of the bradyrhizobium LX-JX01 bacterial agent of the present invention comprises the steps of liquid DM medium preparation, solid DM medium preparation, strain activation and rhizobia culture. The strain can nodulate symbiosis with Dalbergia balata. When Dalbergia japonica LX-JX01 bacterial agent of the present invention was used to plant Dalbergia japonica seedlings, its nodule nodule number increased by 3823% compared with the control, the fresh weight of nodules increased by 1038%, the dry weight of the aboveground part of the plant increased by 47.22%, and the seedling height and plant diameter are increased by 1030% and 42.3% respectively, therefore, the bacterial agent of the present invention can promote the growth of Dalbergia balsamicum. Therefore, the fungal agent of the present invention has broad application prospect in the plantation of Dalbergia balsamanii artificial forest.

Description

【Technical field】 [0001] The invention belongs to the field of biotechnology. More specifically, the present invention relates to a kind of rhizobia, and also relates to the use of said rhizobia. 【Background technique】 [0002] Rhizobia can nodulate and symbiotically fix nitrogen with legumes. Rhizobium fixes nitrogen in the air and converts it into ammonia, thereby providing nitrogen nutrition for legumes, and legumes provide nutrients for rhizobia. The two form a symbiotic system. Due to the specificity and effectiveness of rhizobia in the infection of legumes, it is necessary to select and breed rhizobia suitable for legumes to achieve the symbiotic effect. Inoculation of rhizobia is an internationally recognized biological nitrogen fixation technology. Biological nitrogen fixation will not cause any pollution to the environment, while improving soil and promoting plant growth. It is an important measure for sustainable agricultural development. [0003] Dalbergia Dalbe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/01
Inventor 王琰李春艳孙杉杉苏海榆杜迎辉徐志文
Owner 领先生物农业股份有限公司
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