Facultatively autotrophic sulfur oxidation denitrification rhizobium F43b and application thereof

A rhizobia and sulfide technology, applied in the field of microorganisms, can solve the problems of no sulfur oxidation and denitrification

Active Publication Date: 2016-09-07
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the sediment, a strain was isolated by Shinichi Kaiya in the microenvironment of polychlorinated dioxins, named Rhizobium naphthalenivorans, but it has no sulfur oxidation and denitrification functions

Method used

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  • Facultatively autotrophic sulfur oxidation denitrification rhizobium F43b and application thereof
  • Facultatively autotrophic sulfur oxidation denitrification rhizobium F43b and application thereof
  • Facultatively autotrophic sulfur oxidation denitrification rhizobium F43b and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Rhizobium sp. F43b T separation and identification of

[0018] Take a sample of 10g of river bed sediment in an electronic waste treatment area in Ronggui, Shunde, Guangdong Province, remove residues and large particles, wash with sterile water and centrifuge three times, collect it into a sterilized 120mL headspace bottle, add 100mL enrichment Medium (NH 4 Cl0.5g, MgCl 2 ·6H 2 O 0.5g, KH 2 PO 4 2g, Na 2 S 2 o 3 ·5H 2 O 2g, KNO 3 2g, NaHCO 3 1g, FeSO 4 ·7H 2 O0.1g, 1000mL deionized water, natural pH, autoclave at 121°C for 20min; among them, NaHCO 3 Filter sterilized, MgCl 2 ·6H 2 O. KH 2 PO 4 and FeSO4·7H 2 O Separately configure the mother liquor for sterilization, add it according to the concentration requirements after cooling; add 20g / L agar to the solid medium), press the cap of the headspace bottle, and culture it statically in an anaerobic box at 30°C. One month later, the culture was shaken, and 20 mL of the culture was centrifu...

Embodiment 2

[0024] Example 2: Rhizobium sp. F43b T Sulfur oxidation and denitrification under anaerobic and autotrophic conditions

[0025] Rhizobium sp. F43b T and type strain Rhizobium rosettiformans W3 T and Rhizobium daejeonense L61 T Activate on the rhizobia medium, take a certain amount of bacteria and wash it three times with the sterilized enrichment medium, respectively take 100μL of the bacterial solution with a protein concentration of about 100μg / mL and inoculate it into the sterilized culture medium containing 100mL In the 120mL headspace bottle of base, wherein, the culture medium of this embodiment is the KNO of enrichment medium of embodiment 1 3 The concentration was adjusted to 1g / L, and the other components of the medium remained unchanged. Three replicates were set for each strain, and three blank control groups without inoculation were set. . The culture was shaken once every 12 hours, and samples were taken at 0 days, 2.5 days, 5 days, 7.5 days, 10 days and 12.5...

Embodiment 3

[0027] Example 3: Rhizobium sp. F43b T Sulfur oxidation and denitrification function under organic anaerobic conditions

[0028] Rhizobium sp. F43b T (i.e. strain F43b T ) and type strain Rhizobium rosetiformans W3 T and Rhizobium daejeonense L61 T Activate on the rhizobia culture medium, take a certain amount of bacteria and wash with sterile water three times, take 100 μL of the bacterial solution with a protein concentration of about 100 μg / mL and inoculate it into 120 mL headspace containing 100 mL of medium that has been sterilized In the bottle, design three processing, the medium of processing 1 is to add Na simultaneously in the enrichment medium of embodiment 1 2 S 2 o 3 ·5H 2 O 1g / L and NaAc·3H 2 O 1g / L is used as electron donor, and the culture medium of processing 2 is to add NaAc·3H separately in the enrichment medium of embodiment 1 2 O 1g / L is made electron donor, and the substratum of processing 3 is to add Na simultaneously in the enrichment substratu...

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Abstract

The invention discloses a facultatively autotrophic sulfur oxidation denitrification rhizobium F43b and an application thereof. The Rhizobium sp. F43b<T> is preserved in China Center for Type Culture Collection (CCTCC) Wuhan University in Wuhan in China on March 30, 2016 with the preservation number of CCTCC NO:M 2016158. The Rhizobium sp. F43b<T> is facultatively autotrophic rhizobium with sulfur oxidation and denitrification functions, can remove COD, nitrates and sulfides from polluted water or deposits, realizes simultaneous removal of carbon, nitrogen and sulfur from the polluted water or deposits, and provides bacterial strains and microbial preparations for river restoration and carbon, nitrogen and sulfur wastewater treatment, and researches of the rhizobium provide theoretic guidance for river restoration and removal of carbon, nitrogen and sulfur.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a facultative autotrophic sulfur-oxidizing and denitrifying rhizobia F43b and an application thereof. Background technique: [0002] In an anaerobic environment, due to the lack of sufficient oxygen as electron acceptors, organic matter is often difficult to be oxidized and removed. At the same time, the entry of sulfate provides conditions for sulfate-reducing bacteria (SRB). These SRB can use organic matter as energy and carbon sources, and sulfate as electron acceptors to reproduce in large numbers. Although part of the organic matter can be removed, sulfate reduction also produces a large amount of sulfide. These sulfides are not only an important reason for the black and smelly water body, but also are biologically toxic and corrosive, which will endanger the health of water body organisms, lead to the reduction of biodiversity, and further deteriorate the environm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C02F11/02C12R1/41
CPCB09C1/10C02F3/34C02F11/02C12N1/205C12R2001/41Y02A20/402
Inventor 许玫英邓通初陈杏娟
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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