Rhizobium capable of promoting hairy vetch growth and application thereof

A technology of rhizobia of vetch and pea, which is applied in the field of agricultural microorganisms, can solve the problems of large differences in inoculation effects, achieve high practicability and scalability, broad application prospects, and significant yield-increasing effects

Active Publication Date: 2017-09-15
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Vetch and pea are mutual races in the application of rhizobia. Rhizobium pea can be inoculated with different varieties of vetch and pea, but the inoculation effect is quite different

Method used

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  • Rhizobium capable of promoting hairy vetch growth and application thereof
  • Rhizobium capable of promoting hairy vetch growth and application thereof
  • Rhizobium capable of promoting hairy vetch growth and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1, Isolation and Identification of Pea Rhizobium (Rhizobium leguminosarum) m1-10-3CGMCC11877

[0037] 1. Isolation of strains

[0038] Collect wild vetch root nodules from Ping'an District, Haidong City, Qinghai Province, use a flat plate (get mannitol 10g, yeast extract powder 1g, dipotassium hydrogen phosphate 0.5g, calcium sulfate 0.2g, magnesium sulfate 0.2g, sodium chloride 0.1g, 1ml of ammonium molybdate aqueous solution with a mass content of 1% and 1ml of boric acid aqueous solution with a mass content of 1%, 1ml of 0.5% Congo red, 20g of agar, dilute to 1L with water, and the pH value is 6.8-7.0) to obtain strain m1 by streaking -10-3.

[0039] 2. Identification of strains

[0040] 2.1. Morphological identification

[0041] The strain m1-10-3, which is in the logarithmic growth phase and the colony size is stable, and isolated and purified in the above step 1, is described as a single colony state, mainly including the size, color, transparency, humi...

Embodiment 2

[0048] Embodiment 2, the preparation of pea rhizobium m1-10-3 microbial agent

[0049] 1. Slope culture of pea rhizobium m1-10-3

[0050] Rhizobium pea m1-10-3 of Example 1 was picked and inoculated on a solid medium for slant culture, and cultured at 28° C. for 56 hours to obtain Rhizobium pea m1-10-3 cultured on a slant.

[0051] 2. Activation of bacteria

[0052] Pick the Rhizobium pea m1-10-3 cultured on the slant in step 1, inoculate it in 500 mL of liquid medium, and culture it at 28° C. for 48 hours to obtain the Rhizobium pea m1-10-3 bacterium liquid.

[0053] 3. Seed tank cultivation

[0054] Take 3L of the pea rhizobium m1-10-3 bacteria liquid in step 2, put it into a 100L seed tank (containing 60L liquid medium) for seed culture, and culture it for 56 hours under shaking at 30°C and 120rpm to obtain pea root nodules Bacteria m1-10-3 seed solution.

[0055] 4. Fermentation tank culture

[0056] Put the pea rhizobium m1-10-3 seed solution in step 3 into a 1000L f...

Embodiment 3

[0060] Embodiment 3, the hydroponic test of pea rhizobia m1-10-3 inoculated with V. tomentosa

[0061] The filter paper bridge test tube hydroponics method was used. The filter paper is cut into strips to make an M shape, and the middle recess is made into a V-shaped hole according to the size of the seedlings. The height of the M-type filter paper is the length of the test tube minus 4cm. The test tube used is 2.0cm×20cm.

[0062] Put the prepared nitrogen-free nutrient solution into test tubes with filter paper supports, the height of which is located in the recess of the H-shaped filter paper, repeat 4 test tubes for each treatment, and set 4 control tubes, after filling, use durable After the high-temperature and high-pressure plastic film is sealed, 15 pounds of sterilized at 121°C for 1 hour is ready for use.

[0063] Select the Turkmen vetch seeds with uniform size, soak them in 95% ethanol for 5min, and then wash them with 0.1% HgCl 2 The surface was sterilized for ...

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Abstract

The invention discloses rhizobium leguminosarum capable of promoting hairy vetch growth and an application thereof. The strain number of the rhizobium leguminosarum is m1-10-3, and the preservation number of the rhizobium leguminosarum in the General Microbiology Center of the China Committee for Culture Collection of Microorganisms is CGMCC No.11877. An experiment proves that the plant height and the fresh weight ratio of the hairy vetch inoculated with the rhizobium leguminosarum m1-10-3 are obviously improved in comparison with those of the hairy vetch which is not inoculated with the rhizobium, the hairy vetch which is inoculated with rhizobium leguminosarum H10 and the hairy vetch which is inoculated with rhizobium leguminosarum ACCC16505. The rhizobium leguminosarum has a wide application prospect field in the hairy vetch planting industry.

Description

technical field [0001] The invention relates to a rhizobia strain for promoting the growth of Chinese vetch in the field of agricultural microorganisms and its application. Background technique [0002] Vicia villosa Roth (Vicia villosa Roth, also known as Vicia villosa Roth, referred to as villosa Roth) is a high-quality green manure crop, mainly distributed in the Yellow River, Huaihe River, and Haihe River basins in my country. In recent years, Liao Nian, Inner Mongolia, Xinjiang and other places There are also sowing, and the planting area is larger. The cold-resistant ability of the vetch is strong, and the general varieties can withstand the low temperature of minus 20°C for a short time, and the overwintering rate of the seedlings is very high. At the same time, the vetch is also very resistant to drought and barrenness. It can also receive higher output and has wider adaptability. [0003] In order to fertilize the soil and ensure the sustainable and stable developm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P21/00C05F11/08C12R1/41
CPCA01N63/00A01N63/10C05F11/08C12N1/20C12N1/205C12R2001/41
Inventor 曹卫东马晓彤韩梅张宏亮王雪翠
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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