Rhizobium capable of promoting hairy vetch growth and application thereof
A technology of rhizobia of vetch and pea, which is applied in the field of agricultural microorganisms, can solve the problems of large differences in inoculation effects, achieve high practicability and scalability, broad application prospects, and significant yield-increasing effects
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Embodiment 1
[0036] Example 1, Isolation and Identification of Pea Rhizobium (Rhizobium leguminosarum) m1-10-3CGMCC11877
[0037] 1. Isolation of strains
[0038] Collect wild vetch root nodules from Ping'an District, Haidong City, Qinghai Province, use a flat plate (get mannitol 10g, yeast extract powder 1g, dipotassium hydrogen phosphate 0.5g, calcium sulfate 0.2g, magnesium sulfate 0.2g, sodium chloride 0.1g, 1ml of ammonium molybdate aqueous solution with a mass content of 1% and 1ml of boric acid aqueous solution with a mass content of 1%, 1ml of 0.5% Congo red, 20g of agar, dilute to 1L with water, and the pH value is 6.8-7.0) to obtain strain m1 by streaking -10-3.
[0039] 2. Identification of strains
[0040] 2.1. Morphological identification
[0041] The strain m1-10-3, which is in the logarithmic growth phase and the colony size is stable, and isolated and purified in the above step 1, is described as a single colony state, mainly including the size, color, transparency, humi...
Embodiment 2
[0048] Embodiment 2, the preparation of pea rhizobium m1-10-3 microbial agent
[0049] 1. Slope culture of pea rhizobium m1-10-3
[0050] Rhizobium pea m1-10-3 of Example 1 was picked and inoculated on a solid medium for slant culture, and cultured at 28° C. for 56 hours to obtain Rhizobium pea m1-10-3 cultured on a slant.
[0051] 2. Activation of bacteria
[0052] Pick the Rhizobium pea m1-10-3 cultured on the slant in step 1, inoculate it in 500 mL of liquid medium, and culture it at 28° C. for 48 hours to obtain the Rhizobium pea m1-10-3 bacterium liquid.
[0053] 3. Seed tank cultivation
[0054] Take 3L of the pea rhizobium m1-10-3 bacteria liquid in step 2, put it into a 100L seed tank (containing 60L liquid medium) for seed culture, and culture it for 56 hours under shaking at 30°C and 120rpm to obtain pea root nodules Bacteria m1-10-3 seed solution.
[0055] 4. Fermentation tank culture
[0056] Put the pea rhizobium m1-10-3 seed solution in step 3 into a 1000L f...
Embodiment 3
[0060] Embodiment 3, the hydroponic test of pea rhizobia m1-10-3 inoculated with V. tomentosa
[0061] The filter paper bridge test tube hydroponics method was used. The filter paper is cut into strips to make an M shape, and the middle recess is made into a V-shaped hole according to the size of the seedlings. The height of the M-type filter paper is the length of the test tube minus 4cm. The test tube used is 2.0cm×20cm.
[0062] Put the prepared nitrogen-free nutrient solution into test tubes with filter paper supports, the height of which is located in the recess of the H-shaped filter paper, repeat 4 test tubes for each treatment, and set 4 control tubes, after filling, use durable After the high-temperature and high-pressure plastic film is sealed, 15 pounds of sterilized at 121°C for 1 hour is ready for use.
[0063] Select the Turkmen vetch seeds with uniform size, soak them in 95% ethanol for 5min, and then wash them with 0.1% HgCl 2 The surface was sterilized for ...
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