Rhizobium and application thereof
A technology of bradyrhizobium and bacterial agent, applied in the field of microorganisms, can solve problems such as poor growth status, low content of astragalus polysaccharides, and less nodules of astragalus
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Embodiment 1
[0050] Embodiment 1, the isolation and identification of rhizobia
[0051] 1. Separation
[0052] 1. Disinfection
[0053] (1) Take the root nodules of Astragalus membranaceus plants planted in Guaner Township, Hunyuan County, Shanxi Province, wash them with sterile normal saline with a concentration of 0.8g / 100mL, and then wash them with sterile normal saline with a concentration of 0.8g / 100mL Soak until fully expanded, and the soaking temperature is 4°C.
[0054] (2) After step (1), the root nodules are taken, placed in a sterile centrifuge tube, and soaked in an appropriate amount of 95% (v / v) ethanol aqueous solution for 45 seconds.
[0055] (3) After completing step (2), the liquid phase was discarded, and an aqueous sodium hypochlorite solution with a concentration of 2.4 g / 100 mL was added to the sterile centrifuge tube until the root nodules were completely submerged, and then sterilized for 5 minutes.
[0056] (4) After completing step (3), discard the liquid phase...
Embodiment 2
[0065] Embodiment 2, the application of rhizobia
[0066] One, the preparation of rhizobia bacterial agent
[0067] CCBAU 03524 bacterial agent: Activate the strain CCBAU 03524 on a YMA solid plate, then pick a single colony and inoculate it in a 500mL Erlenmeyer flask containing 200mL YMA liquid medium, and culture it with shaking at 28°C and 160rpm / min for 3 days to obtain OD 600nm Value is 0.8~1.0 culture bacteria liquid; In described culture bacteria liquid, add trace element solution (1 *) and carboxymethyl cellulose sodium aqueous solution, obtain CCBAU 03524 bacterial agent; In CCBAU 03524 bacterial agent, trace element solution ( The concentration of 1×) is 1 mL / L, and the concentration of sodium carboxymethyl cellulose is 1 g / 100 mL.
[0068] According to the preparation method of the above-mentioned CCBAU 03524 bacterial agent, the bacterial strain CCBAU 03524 was replaced with the bacterial strain 01583 to obtain the 01583 bacterial agent.
[0069] According to th...
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