Rhizobium and application thereof

A technology of bradyrhizobium and bacterial agent, applied in the field of microorganisms, can solve problems such as poor growth status, low content of astragalus polysaccharides, and less nodules of astragalus

Inactive Publication Date: 2017-04-26
CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Astragalus membranaceus is mostly planted in deserts and barren mountain areas with poor soil, and in these areas, artificial fertilization is required to promote the growth of Astragalus membranaceus, and there are many problems such as lack of fertilizer sources and difficulty in fertilization. At the same time, most of the planting areas are newly reclaimed There is a lack of matching rhizobia in the soil, and even if they exist, they are relatively small in number. Therefore, artificially cultivated Astragalus has problems such as less nodules, poor growth status, and low content of Astragalus polysaccharides.

Method used

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  • Rhizobium and application thereof
  • Rhizobium and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1, the isolation and identification of rhizobia

[0051] 1. Separation

[0052] 1. Disinfection

[0053] (1) Take the root nodules of Astragalus membranaceus plants planted in Guaner Township, Hunyuan County, Shanxi Province, wash them with sterile normal saline with a concentration of 0.8g / 100mL, and then wash them with sterile normal saline with a concentration of 0.8g / 100mL Soak until fully expanded, and the soaking temperature is 4°C.

[0054] (2) After step (1), the root nodules are taken, placed in a sterile centrifuge tube, and soaked in an appropriate amount of 95% (v / v) ethanol aqueous solution for 45 seconds.

[0055] (3) After completing step (2), the liquid phase was discarded, and an aqueous sodium hypochlorite solution with a concentration of 2.4 g / 100 mL was added to the sterile centrifuge tube until the root nodules were completely submerged, and then sterilized for 5 minutes.

[0056] (4) After completing step (3), discard the liquid phase...

Embodiment 2

[0065] Embodiment 2, the application of rhizobia

[0066] One, the preparation of rhizobia bacterial agent

[0067] CCBAU 03524 bacterial agent: Activate the strain CCBAU 03524 on a YMA solid plate, then pick a single colony and inoculate it in a 500mL Erlenmeyer flask containing 200mL YMA liquid medium, and culture it with shaking at 28°C and 160rpm / min for 3 days to obtain OD 600nm Value is 0.8~1.0 culture bacteria liquid; In described culture bacteria liquid, add trace element solution (1 *) and carboxymethyl cellulose sodium aqueous solution, obtain CCBAU 03524 bacterial agent; In CCBAU 03524 bacterial agent, trace element solution ( The concentration of 1×) is 1 mL / L, and the concentration of sodium carboxymethyl cellulose is 1 g / 100 mL.

[0068] According to the preparation method of the above-mentioned CCBAU 03524 bacterial agent, the bacterial strain CCBAU 03524 was replaced with the bacterial strain 01583 to obtain the 01583 bacterial agent.

[0069] According to th...

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Abstract

The invention discloses a rhizobium and application thereof. The rhizobium provided by the invention is northern Mesorhizobium septentrionale CCBAU 03524, of which the preservation number in the China General Microbiological Culture Collection Center is CGMCC No.11015. Experiments show that the northern Mesorhizobium septentrionale CCBAU 03524 CGMCC No.11015 has important application value in promoting growth of astragalus plants and/or improving quality of astragalus and/or raising seedlings of astragalus plants; the promotion of the growth of astragalus plants is reflected as increase of the number of nodules and/or increase of dry weight of underground parts; the improvement of the quality of astragalus is reflected as increase of percentage of astragalus polysaccharide in astragalus root.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of rhizobia and its application. Background technique [0002] Rhizobia are nitrogen-fixing bacteria that can nodulate and symbiose with legumes. When rhizobia live in symbiosis with suitable legumes, the rhizobia fix nitrogen in the air as ammonia, thereby providing nitrogen nutrition to the legumes, and the legumes provide carbohydrates and energy to the rhizobia through photosynthesis, both form a good symbiotic relationship. [0003] Since there is mutual recognition between rhizobia and legumes, and this effect is affected by geographical environment and soil physical and chemical factors, it is necessary to select and breed rhizobia suitable for legumes and adapt to the environment in order to achieve the difference between the two. A symbiotic effect of mutual benefit. Planting legumes in soil that has never been planted with legumes requires inoculation of suitab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00A01G9/10C12R1/01
CPCA01N63/00A01G9/029C12N1/205C12R2001/01
Inventor 陈文峰严慧曹秀娟侯美利
Owner CHINA AGRI UNIV
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