Fulvic acid and rhizobium biological type leguminous plant seed coating agent and preparation method thereof
A technology of fulvic acid root nodules and leguminous plants, applied in botany equipment and methods, seed coating/seed dressing, plant growth regulators, etc., can solve the problems of difficult survival time of rhizobia and poor competitiveness , to achieve the effect of improving the ability of nodulation and nitrogen fixation, reducing the amount of use, and enhancing the activity
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Embodiment 1
[0041] Using lignite, sawdust, and wheat bran as raw materials, the degraded fulvic acid obtained by fermenting the degrading bacteria Penicillium and Bacillus, extracting with water, and drying at low temperature is used for future use.
[0042] The brady-growing soybean rhizobia was cultured in YMA medium in seed tanks at 28°C for 3 days, then fermented in fermenters under the same conditions for 7 days, and the number of bacteria reached 3.3×10 9 The soybean rhizobia culture fluid of cfu / ml, standby,
[0043]Add 5kg of hydroxypropyl methylcellulose into 86.47kg of water and heat it to 85°C to slowly dissolve it, then add 3kg of sodium dodecylbenzenesulfonate and dissolve it, then add 0.5kg of molybdate and 0.03kg of yellow Humic acid, cooled to 25° C., added 5 kg of the soybean rhizobium culture solution prepared above, and mixed uniformly to obtain the fulvic acid rhizobium biotype legume seed coating agent product.
[0044] 30kg of soybean seeds are mixed with 1kg of the...
Embodiment 2
[0046] The degraded fulvic acid obtained by using weathered coal, sawdust and wheat bran as raw materials, fermented by the degrading bacteria Penicillium and Bacillus, extracted with water, and dried at low temperature, is ready for use
[0047] Mung bean rhizobia were cultured in YMA medium on a shaking table at 28°C for 4 days, then put into a fermenter and fermented under the same conditions for 6 days, and the number of bacteria reached 4×10 9 Mung bean rhizobia culture solution of cfu / ml, spare
[0048] Add 6kg of polyvinyl alcohol into 85.2kg of water and heat it to 83°C to slowly dissolve it, then add 4kg of cellulose derivatives and dissolve it, add 0.7kg of sulfate and 0.1kg of fulvic acid prepared above, cool to 28°C, Add 4kg of the rhizobia culture solution prepared above, and mix evenly to obtain the fulvic acid rhizobia biotype legume seed coating agent product.
[0049] 50kg of mung bean seeds are mixed with 1 kg of the above-mentioned product, and air-dried in...
Embodiment 3
[0051] Coal gangue, sawdust, and wheat bran are used as raw materials, and after being fermented by the degrading bacteria Penicillium and Bacillus, they are extracted with water, and the obtained liquid degrades fulvic acid for future use.
[0052] Use YMA medium for Rhizobium meliloti, culture in the seed tank at 28°C for 5 days, put it into the fermenter and ferment for 5 days under the same conditions, and the number of bacteria reaches 4.5×10 9 Cfu / ml peanut rhizobia culture solution, for later use
[0053] Add 3kg of chitosan and 4kg of cellulose ether into 82.5kg of water and heat it to 80°C to dissolve slowly, then add 5kg of polyacrylamide and dissolve it, then add 0.5kg of sulfate, 0.5kg of mannitol and 0.5kg of the above prepared yellow Humic acid, cooled to 25°C, added 4kg of rhizobia culture solution prepared above, mixed evenly, to obtain fulvic acid rhizobia biotype leguminous plant seed coating agent product.
[0054] 40kg of alfalfa seeds are mixed with 1kg o...
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