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Plant growth-promoting rhizobacteria and application thereof

A rhizobia and growth-promoting agent technology, applied in the field of agricultural microorganisms, can solve problems affecting various enzyme functions and activities, affecting cell membrane functions, affecting plant photosynthesis, respiration root tip elongation, etc., to prevent and control apple continuous cropping obstacles, The effect of high phlorizin degradability

Active Publication Date: 2018-04-20
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) Affect the function of cell membrane and inhibit the absorption of nutrients by plants. The damage of phenolic allelochemicals to cell membrane may be the starting point of the autotoxic effect produced by allelochemicals; (2) Affect the functions and activities of various enzymes ; (3) Affect photosynthesis, respiration and elongation of root tips of plants

Method used

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  • Plant growth-promoting rhizobacteria and application thereof
  • Plant growth-promoting rhizobacteria and application thereof
  • Plant growth-promoting rhizobacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the acquisition of rhizobia

[0029] From the yellow river delta saline-alkali soil planting area, select the vigorous growth of the above-ground and underground parts, collect the root nodules, smash the root nodules, and spread the leachate on the YMA plate, and culture it upside down at 28°C for 3-5 days, until it grows out For round colonies with neat edges, milky white translucence, and more exopolysaccharides, pick a single colony and streak repeatedly until it is purified (see figure 1 ).

[0030] The formula of YMA solid medium (yeast extract mannitol agar medium) is: mannitol 10g / L, yeast extract 3g / L, K 2 HP0 4 0.25g / L, KH 2 P0 4 0.25g / L, MgSO 4 0.2g / L, CaCO 3 3g / L, NaCl 0.1g / L, dilute to 1000mL with deionized water, and divide them into Erlenmeyer flasks, and add agar powder with a final concentration of 20g / L to each bottle.

[0031] (1) Identification of Rhizobium

[0032] Gram staining and morphological identification: Gram stainin...

Embodiment 2

[0041] The growth-promoting effect of embodiment 2 rhizobia YIC4105 to apple seedling

[0042] (1) Strain activation

[0043] Pick up YIC4105 colony with a sterilized inoculation loop, and streak it on YMA solid medium, and culture it upside down at 28°C-32°C for 2-3 days until the colony grows well.

[0044] (2) Preparation of bacteria agent

[0045] Use a sterilized inoculation loop to pick one or two loops of bacteria, inoculate into 200mL sterilized liquid TY liquid medium, culture at 28°C-32°C, 180-200r / m shaking, and cultivate until OD600 is about 1.5 . Put the bacterial solution in a refrigerator at 4°C as a bacterial agent for use.

[0046] (3) Apple seeds are stored in sand at 4°C at low temperature, keeping the sand moist. After about 30 days, the seeds germinate, transplanted into sterilized soil to settle the seedlings, and placed in an artificial climate room for cultivation. The light cycle is light 12h / dark 12h, and the light intensity is 600μmol m -2 ·s -...

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Abstract

The invention relates to the field of agricultural microorganisms, and in particular relates to a rhizobium pusense capable of degrading apple autotoxin phlorizin and application thereof. A rhizobiumpusense YIC4105 is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 2rd, 2017 with the preservation number of CGMCC No.14852. The bacterial strain is separatedfrom sesbania rhizosphere soil naturally distributed at the Yellow River delta and is obtained through artificial separation and purification; the bacterial strain has relatively strong capability ofdegrading phlorizin and can be stabilized and colonized at rhizosphere of apples; and successive cropping obstacle of an apple seedling caused by accumulation of phlorizin can be effectively preventedby utilizing the bacterial strain, so that the rhizobium provided by the invention has good prospect of being developed into a microbiological inoculants or bacterial fertilizer capable of promotingcrop growth.

Description

technical field [0001] The invention relates to the field of agricultural microorganisms, in particular to a rhizobia for degrading apple autotoxic substance phlorizin and application thereof. Background technique [0002] Apple belongs to the Rosaceae apple genus and is the largest fruit variety in my country, with a cultivated area of ​​about 200km 2 , the cultivated area and output account for 40% of the world's total. Apple continuous cropping obstacles are a major problem faced by the apple industry. Continuous cropping apple seedlings have a low survival rate and poor root development, which seriously restricts the yield and quality of apples. [0003] The currently generally recognized mechanism of continuous cropping obstacles is the result of the combined effects of soil fertility decline, microbial community structure imbalance, and allelopathic effects. The countermeasures for continuous cropping obstacles mainly include crop rotation, increasing organic fertili...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01G7/06A01N63/00A01P1/00A01P21/00C05G3/00C05F11/08C12R1/41
CPCA01G7/06A01N63/00C05F11/08C05G3/00C12N1/205C12R2001/41
Inventor 任承钢李岩解志红刘卫阿孜古力.买买提李项岳孔存翠
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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