New applications of Rhizobium W33 strain

A rhizobia and utilization technology, applied in agricultural technology and rhizobia application fields, can solve the problems of secondary pollution of wrapping materials, slow release, and incompatibility with complex soil ecological environment.

Active Publication Date: 2016-03-23
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are several problems in the above-mentioned technologies, such as slow-acting potassium fertilizers, which often use polymeric materials to wrap potassium sulfate, etc., the release is very slow and difficult to meet the need

Method used

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  • New applications of Rhizobium W33 strain
  • New applications of Rhizobium W33 strain
  • New applications of Rhizobium W33 strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Bacterial strain W33 is to the uptake capacity of K in the solution

[0017] Prepare medium (sucrose 10.0g, (NH 4 ) 2 SO 4 0.5g, Na 2 HPO 4 2.0g, NaCl0.1g, KCl0.038g, 0.115g or 0.191g, MgSO 4 ·7H 2 O0.5g, H 2 (2000ml), wherein the KCl addition is 0.038g, 0.115g and 0.191g respectively, so that the KCl content is set to 20%, 60% and 100% K, adjusts pH7.0-7.5, and sterilizes at 115°C for 30min. W33 (CCTCCNO: M2012357) was inoculated in the above liquid medium, and cultured statically at 30° C. for 3 days. Sampling for determination of W33 (CCTCCNO: M2012357) OD in fermentation broth 600 value, the fermentation broth was centrifuged at 12000rpm for 5min, the supernatant was taken to measure the K element content, and the K uptake rate of the bacteria was calculated. It can be seen from Table 1 that under the conditions of 20%, 60% and 100% K content, the OD in the fermentation broth 600 The value reaches about 0.8, and there is no difference, indicating...

Embodiment 2

[0020] Activation of embodiment 2 bacterial strain W33 and bacterial suspension preparation

[0021] The W33 (CCTCCNO: M2012357) slant strain was inoculated on a solid medium (sucrose 10.0 g, (NH 4 ) 2 SO 4 0.5g, Na 2 HPO 4 2.0g, NaCl0.1g, KCl0.191g, MgSO 4 ·7H 2 O0.5g, H 2 O1000ml, agar 20g), cultured at 30°C for 3d. Then choose plump, viscous W33 colonies and inoculate them in liquid medium (sucrose 10.0g, (NH 4 ) 2 SO 4 0.5g, Na 2 HPO 4 2.0g, NaCl0.1g, KCl0.191g, MgSO 4 ·7H 2 O0.5g, H 2 O1000ml), shake at 150rpm at 30°C for 16‐20h. Transfer the fermentation broth to a sterile centrifuge bottle, centrifuge at 5000 rpm for 5 minutes to collect the bacteria, wash with sterile deionized water, and resuspend to make the number of cells reach more than 500 million CFU / ml.

Embodiment 3

[0022] Example 3 Inoculation of soil with bacterial strain W33 reduces K leaching loss

[0023] A simulated soil column experiment was used. The soil column experiment uses a PVC pipe with a height of 35 cm and an inner wall diameter of 4.5 cm. The lower end of the pipe is wrapped with nylon mesh, filter paper, and nylon mesh in sequence, and 30 g of clean quartz sand is filled; take a soil sample of 0-20 cm of yellow-brown soil vegetable garden soil, Air-dried through a 5mm aperture sieve, and then loaded into PVC pipes, each soil column was filled with a total of 400g of air-dried soil samples. Place a piece of filter paper with a diameter of 42 mm on the surface of the soil column to ensure that the surface is smooth and the water can evenly penetrate into the soil column when water is added. Add 160ml of water to adjust the water content of the soil column to 60% of the saturated water holding capacity in the field. After standing for 24 hours, press 0.241gK 2 Fertilizer...

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Abstract

The present invention belongs to the fields of agriculture and agricultural technology and Rhizobium applications, and discloses new applications of a Rhizobium sp. W33 strain. According to the present invention, the Rhizobium sp. W33 is adopted as a raw material, and bacterial activation is performed to prepare a bacterial suspension, wherein the bacterial suspension is inoculated in a potassium-containing liquid culture medium so as to absorb the potassium in the solution, and the bacterial suspension is inoculated in soil so as to reduce potassium fertilizer leaching loss, significantly promote plant growth and absorption on potassium and phosphorus, and improve potassium fertilizer absorption and utilization rate.

Description

technical field [0001] The invention belongs to the fields of agricultural technology and application of rhizobia, and relates to the new application of Rhizobium sp. W33 strain, in particular to the application of Rhizobium sp. W33 strain in improving the absorption and utilization rate of plant potassium fertilizer. Background technique [0002] Potassium fertilizer resources are lacking in my country, and the import dependence rate is high. Potassium fertilizer input in agricultural production is prone to leaching, and the utilization rate is only 20% to 60%. As a result, my country's agriculture spends a lot of foreign exchange on importing potassium fertilizer. The more it is used in production, the more leaching will be. , The more serious the soil structure damage, the vicious cycle of crop yield and quality decline. Internationally, the comprehensive management technology of nutrient resources has been proposed, emphasizing the strategy of reducing chemical fertilizer...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05G3/04C12N1/20C12R1/41C05G3/80
CPCY02A40/10
Inventor 何琳燕盛下放黄智闫亚南
Owner NANJING AGRICULTURAL UNIVERSITY
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