Rhizobium strain and application thereof

A rhizobia agent, magnolia rhizobia technology, applied in the direction of bacteria, organic fertilizers, microorganisms, etc., can solve the problems of not necessarily long survival time and reduced infection ability

Inactive Publication Date: 2011-10-12
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, a large number of studies have shown that the inoculation of rhizobia can increase the nodulation number of a single green manure plant and enhance the nitrogen fixation efficiency, but its survival time is not neces...

Method used

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  • Rhizobium strain and application thereof
  • Rhizobium strain and application thereof
  • Rhizobium strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. Isolation and Identification of Magnolia Rhizobium (Rhizobium indigoferae) NM60CGMCC No.4647

[0027] 1. Isolation and purification of strains:

[0028] Collect wild pea root nodules from the Inner Mongolia area, select the pink nodules, wash them, and then disinfect them with 3% sodium hypochlorite for 5 minutes, rinse them with sterile water several times, and transfer them to the rhizobium agar (I) slant with sterile tweezers Gently crush the root nodules and culture them by streaking. Separation and purification of bacterial strains with rhizobia agar (I) plate method, picking neat edges, milky white, translucent colony, Gram staining, microscopic examination, as impure, repeated plate streaking purification, strain number is NM60.

[0029] Medium and composition: Rhizobium agar (I)

[0030]

[0031] 2. Identification of NM60 strains:

[0032] Strain morphology observation:

[0033] Cultured in the above medium for 48 hours, the colony is round, mi...

Embodiment 2

[0047]Embodiment 2, the symbiotic nitrogen fixation performance of Rhizobium indigoferae (Rhizobium indigoferae) NM60CGMCC No.4647 and different sweet potato varieties

[0048] 1. Experimental method

[0049] Culture of bacteria

[0050] At first the bacterial strain is activated, the bacterial classification is streaked on the slope of the above-mentioned medium (rhizobium agar (I)), cultivated for 48 hours at 30°C, and the bacterial classification activated is inoculated into 150ml liquid medium ( Except not containing agar, in the 500ml flask that all the other ingredients are identical with Rhizobium agar (I), carry out shaking table culture, culture temperature is 30 ℃, and shaking table rotating speed is 200rpm, cultivates 48 hours, obtains bacterium liquid.

[0051] The seeds of the plants to be tested are the following varieties: Turkmen vetch, vetch, 79-121 vetch, and 78-195. Among them, Turkmen sp. and Vulgaris were selected from the National Germplasm Resource Ban...

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Abstract

The invention discloses a rhizobium strain and an application thereof. The rhizobium strain is rhizobium indigoferae NM60 of which the CGMCC ((China General Microbiological Culture Collection Center) No is 4647. Rhizobium indigoferae NM60 can promote the growth of different kinds of hairy vetches, the strain has better symbiotic nodulation capability with each kind of hairy vetch, the strain is determined as the broad-spectrum nodulation strain of vetch and can be used to prepare green manure; and the strain has higher practicability and popularization and has a wide application prospect in the planting of vetch.

Description

technical field [0001] The invention relates to a strain of rhizobia and its application. Background technique [0002] It is estimated that biological nitrogen fixation provides about 200 million tons of nitrogen for the world every year, 75% of which are fixed by symbiotic nitrogen-fixing organisms, and the symbiosis of rhizobia and leguminous plants is the most effective system among nitrogen-fixing organisms, and the fixed nitrogen accounts for about 100 million tons of biological nitrogen. 65% of the total nitrogen fixation. Almost all leguminous green manures have suitable rhizobia that live in symbiosis with them, forming a large number of nodules on the root system. Under normal circumstances, after establishing a good symbiotic relationship between rhizobia and leguminous green manure, there will be a strong nitrogen fixation effect, which provides abundant and cheap nitrogen nutrition for crops, and plays an important role in fertilizing the soil and improving cro...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/41
Inventor 张晓霞马晓彤顾金刚曹卫东姜瑞波
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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