2 results about "Pandanus tectorius" patented technology

The invention discloses a Pandanus tectorius cutting propagation method, and relates to the technical field of plant tissue propagation, the Pandanus tectorius cutting propagation method comprises the following steps: mixing river sand and red soil according to a volume ratio of (3-6): 1 to prepare a cutting medium, and disinfecting; a robust mother plant of pandanus tectorius is selected, semi-lignified and well-growing branches are cut to serve as cutting slips of the pandanus tectorius, and the base diameter of the cutting slips of the pandanus tectorius is 2-4 cm; the pandanus tectorius cutting slips are soaked with a naphthylacetic acid nano rooting agent; the pandanus tectorius cutting slips soaked with the rooting agent are subjected to cuttage and disinfection. The matrix formed by mixing the river sand and the red soil according to the proportion is good in drainage performance and air permeability, and the moderate humidity can be kept. The epidermis of the pandanus tectorius cutting slips is perforated, so that organic nutrition conveying from top to bottom can be slowed down, calluses are formed at the perforated positions, and the rooting rate is increased. According to the scheme, the rotting condition of the pandanus tectorius cutting slips can be effectively reduced, and the rooting rate of the pandanus tectorius cutting slips is increased.

This invention provides a tissue culture medium for *Pandanus tectorius* leaves and a method for rapid propagation through tissue culture. Using young lateral buds of *Pandanus tectorius* leaves as explants is preferable due to its low contamination rate, rapid initiation of proliferation, and superior surface sterilization method, resulting in low contamination and browning. Adding 0-0.1 mg TDZ and 1-3 mg 6-BA to MS medium for *Pandanus tectorius* leaf bud induction culture rapidly initiates proliferation. Subculture with NAA and 6-BA in MS medium effectively improves the proliferation rate, producing robust and rapidly growing seedlings that effectively balance callus amplification and bud propagation. Adding NAA to 1 / 2 MS medium for *Pandanus tectorius* leaf rooting culture results in high rooting efficiency and good root growth and development. This allows for rapid and large-scale propagation of *Pandanus tectorius* leaf tissue culture seedlings, enabling large-scale production, promoting the vigorous development of *Pandanus tectorius* leaf cultivation, and stimulating the development of related industries such as pharmaceuticals, health foods, and ornamental flowers based on *Pandanus tectorius* leaf raw materials.