74 results about "Vitis davidii" patented technology

The invention discloses a grape cuttage root-forcing growing method, relates to planting of a plant and belongs to the field of agricultural planting. The grape cuttage root-forcing growing method is characterized by comprising the following steps: with an eugenic grape vine as a cutting, stumping and shearing 1cm away from a snip at the upper end of the cutting, shearing 0.5cm close to a knot of the snip at the lower end in an inclined manner, after the cutting is cut, placing the cutting in water formed by diluting fermented glutinous rice for 100-500 times, and soaking for 12-24 hours until the snip is bright green and moisture saturation is achieved; then dipping 1-2cm of the base of the cutting with medicine; and then carrying out cutting seedling outdoors. The grape cuttage root-forcing growing method disclosed by the invention has the beneficial effects that the cutting is soaked by an aqueous solution formed by diluting the fermented glutinous rice for 100-500 times, metabolism of plants can be improved, and synthesis of carbohydrate and growth and development of the plants can be promoted; then 1-2cm of the base of the cutting is dipped with the medicine; and the cutting branch is high in survival rate and takes root quickly. The grape cuttage root-forcing growing method disclosed by the invention is simple in process, low in cost and easy to popularize.

The present invention discloses Vitis davidii Foex juice, Vitis davidii Foex beverage and its preparation process. The preparation process of Vitis davidii Foex beverage includes the following steps: washing and crushing Vitis davidii Foex, color protection, squeezing, filtering, clarifying, blending, packing and sterilizing. The Vitis davidii Foex juice and the Vitis davidii Foex beverage have bright rose color, fruity smell and excellent health function.

The invention discloses a method for preparing two main anthocyanin standard substances in cloudy juice of vitis davidii by utilizing high-speed counter-current chromatography. The method comprises the following steps: enriching the cloudy juice of vitis davidii by adopting a macroporous resin, eluting with ethanol, performing freeze drying to obtain a crude sample, performing high-speed counter-current separation on the crude sample with water in a solvent system, and separating to prepare anthocyanin standard substances, wherein the anthocyanin standard substances are respectively malvidin-[3-O-glucose-]-[5-O-glucose] with purity of 95.8% and malvidin-[3-O-beta-glucosyl-6-coumaroyl]-[5-O-beta-D-glucose] with purity of 92.2%. The yield of the two anthocyanin standards prepared from the cloudy juice of vitis davidii is respectively 122.77mg/L and 78.09 mg/L. A method for separating anthocyanin and polyphenol in the cloudy juice of vitis davidii by high-speed counter-current chromatography is established, and the method for preparing high-purity standard substances can meet large-scale preparation of natural anthocyanin of the vitis davidii.

The invention provides an acquiring and subculture maintaining method for high yield oligomeric proanthocyanidins vitis davidii callus. The method comprises the steps of acquisition of sterile embryo, starting culture, enrichment culture, screening of vitis davidii callus high yield oligomeric proanthocyanidins cell lines, subculture maintaining of the screened high yield oligomeric proanthocyanidins callus in long term and the like. According to the invention, not only can a great amount of vitis davidii callus be obtained quickly, but also the acquired vitis davidii callus has the advantages of high inductive efficiency, exuberant growth and high yield of oligomeric proanthocyanidins; meanwhile, the vitis davidii callus can be conducted by subculture maintaining in long term, that is, exuberant growth capability is provided, so as to lay favorable foundation for study of secondary metabolites of vitis davidii biotechnology and cell culture and production.

The invention relate to a grape planting method. The method for planting ice grapes is: (1) seedlings of grape are planted in a wedge-type groove base, the wedge-type groove base has the size: depth is 140-170cm, the width of upper side is 80-130cm, and the width of the lower side is 40-80cm; (2) organic matter is filled in the wedge-type groove base, wherein, the organic matter consists of plant straws and mull dejecta of animals, and the weight proportion of the organic matter is that: plant straws : mull dejecta of animals is equal to 0.5-2 : 0.5-1; (3) greenhouse raw soil is adopted for fix planting of grapes, and the grapes are coated with paper bags; (4) the grape arbor is provided with double supporting objects which incline to outside by 30-45 degrees; (5) root dripping irrigation is adopted in the growth period; (6) fertilizers are mainly phosphorus fertilizer and kalium fertilizer, and the amount for using phosphorus fertilizer should be increased by 20-30 percent compared with the normal amount; the amount for using kalium fertilizer should be increased by 30-40 percent compared with the normal amount; (7) After being mature, the grapes are left on the bines, after the temperature drops to minus 7-minus 15 DEG C, the fruits are frozen, and the frozen fruits are kept for 10-20 hours, and then picked. In the method of the invention, deep planting is adopted, which can protect the root of grape from being frostbitten. The used fertilizers are mainly phosphorus fertilizer and kalium fertilizer, which can improve the cold-resistance and adherence of grape, thus realizing the volume production for planting ice grapes.

The invention provides a planting method of selenium-rich grapes, and belongs to the technical field of agricultural plantation. The planting method comprises the following steps: pollinating the grapes in an artificial pollination manner, and carrying out primary top-dressing in 5 days after the artificial pollination; integrally sprinkling a primary selenium-rich fungal fertilizer on grape vines on the eighth-twelfth day after the artificial pollination; and sprinkling the primary selenium-rich fungal fertilizer on young fruits with the size of 2-3 mm, wherein the selenium-rich fungal fertilizer is obtained through the following steps: carrying out microbial fermentation on garlic straws, chicken manure, Radix Astragali residues, furfural residues, dried pine needles, eggshells and brown sugar to form a fermented bio-fertilizer and carrying out fungal planting shift on the fermented bio-fertilizer. The applying time of a selenium-rich foliar fertilizer is controlled and the selenium-rich foliar fertilizer is biologically improved to obtain grapes with high selenium content and increase the yield of the grapes.

The invention relates to a vineyard duck goose ecological polyculture plantation method; grapes are planted into a net; cross bars penetrate grapevine gaps, and the grapevines and cross bars can braid a sky net, so the sky net and the ground can form a netted poultry culture loop; the grape fruit bearing height is 1.4-1.6 meters; the netted culture loop is equally divided into culture zones according to the size of the base; each culture zone is provided with channels allowing ducks and gooses to freely pass; the duck and goose stocking density is 20-30/mu. The plantation zones and culture zones are reasonably managed in the grape production process, thus improving grape output and quality; in addition, pesticide sprinkling is not carried out, thus ensuring duck goose safety, fully utilizing waste resources, and promoting ecology circulation.

The invention discloses a grape planting method. The grape planting method comprises following steps: flattening ground and planting by cuttage manner; watering once after grape vines are planted for 6 to 8 hours and keeping ground moisture; pruning vines according to growing states of grape vines in order to keep uniform growth of grape vines and achieve adequate lighting and spraying a nutrient solution to grape vines; and applying fertilizers to grape vines and dressing agricultural organic fertilizers and supplementing topsoil for grape vines. The grape planting method has following beneficial effects: the grape planting method is especially and suitably used in a novel high-quality vineyard so that the daylighting rate of grape vines can be effectively controlled; high efficiency for solar energy utilization is obtained; and obtained grape fruits are affected by a little impact of environmental pollution and are great in market value.

The invention discloses an external applying agent for curing hyperosteogeny, disc herniation, cervical spine inflammation and rheumatoid arthritis, belonging to the geat saamc xiaoy. The invention aims to provide an external applying agent for curing the symptoms such as hyperosteogeny and rheumatoid arthritis. The external applying agent is made by angled bittersweet root, achyranthes, angelica, loosestrife, radix aconiti feri, garden balsam stem and Vitis davidii. The compositions by weight portion are: 30 to 40 portions of angled bittersweet root, 30 to 40 portions of achyranthes, 25 to 30 portions of angelica, 20 to 25 portions of loosestrife, 10 to 15 portions of radix aconiti feri, 15 to 20 portions of garden balsam stem and 30 to 40 portions of Vitis davidii. The method for making the external applying agent is as follows: the raw material medicines are washed off, aired or sun-dried, grinded into powder, and are mixed evenly and then stirred by 100 portions of alcohol with 52 to 56 degrees and are mixed into paste with 40 portions of ripe teal oil. The external applying agent has the advantages of short period of treatment, rapid odynolysis, good healing effect and convenient use, etc., thereby being an ideal medicine for curing symptoms such as hyperosteogeny.

The invention belongs to the technical field of freshness preservation, and especially discloses a freshness preservation method of vitis davidii. The freshness preservation method of the vitis davidii comprises the following four steps of carrying out refrigeration, carrying out spray-coating, carrying out packaging, and carrying out storage. An appropriate preservation agent is adopted so as to produce synergistic freshness preservation effects, and thus, nutritional levels of the vitis davidii are effectively guaranteed, microorganism growth is inhibited, and water loss is reduced; moreover, storage temperature and humidity controls are combined so as to further enhance SOD activity, and thus, senility is delayed and PPO enzyme activity is inhibited so as to prevent occurrence of browning.

The invention discloses a grape grafting seedling cultivation method. The method includes steps of rootstock preparation, cion cutting, cuttage grafting and binding and later-period management. Vitis davidii is treated and grafted according to characteristics of damp resistance, waterlogging resistance and easiness in rooting, rooting speed can be effectively increased while callus tissues can be quickly generated between rootstock and cion, cion buds can acquire sufficient moisture and nutrients after germination, and accordingly the survival rate is effectively increased. By adoption of the method for grape grafting, problems of long production period, low seedling rate and high cost are effectively solved, and the method which is an excellent grape grafting seedling cultivation method has advantages of short seedling cultivation period, low cost, stable effects and the like.

The invention discloses a grape vine planting method which includes the steps of (1) building grape vine supports: sequentially burying bamboo poles 1.5 m-1.6 m in length into the ground, wherein the lengths of the portion, inserted into the ground, of the bamboo poles range from 0.2 m to 0.3 m, the line distances between the bamboo poles range from 2 m to 2.1 m, the row distances between the bamboo poles range from 1 m to 1.5 m, and criss-cross hemp ropes are tied between the bamboo poles in each line; (2) planting seedlings: selecting the grape vine seedlings with two to three buds and the lengths ranging from 6 cm to 10 cm, planting the seedlings at the positions 7 cm-9 cm away from the bamboo poles, thoroughly watering the seedlings, and distributing organic fertilizers after five to six days, wherein the planting depths range from 2 cm to 3 cm; (3) carrying out daily management: when vines are grown out from the seedlings, pulling the vines to the hemp ropes, watering the vines every other day, semimonthly weeding, and timely removing ill and old leaves; (4) carrying out harvesting: removing tender tips and tender leaves after fruits are grown out, sleeving the fruits with paper bags, and taking down the paper bags two weeks before harvesting to carry out harvesting. By means of the grape vine planting method, the hemp ropes are adopted for manufacturing the supports, the planting method is simplified, and the production cost can be saved.

The invention relates to a method for brewing vitis davidii wine. The method comprises the following steps: 1) raw material treatment: selecting full-grained vitis davidii free from plant diseases and insect pests, carrying out soaking with purple perilla water, carrying out washing, then, carrying out draining, and then, removing fruit stems for later use; 2) primary fermentation: placing 200-300 parts of the vitis davidii treated in the step 1), 50-60 parts of crystal sugar and 0.6-0.8 part of yeast in a fermentation cask, carrying out uniform stirring, and carrying out fermentation until the sugar content is 60g/L-70g/L, so as to obtain base wine; and 3) secondary fermentation: adding purple perilla juice into a stirring barrel, then, continuing to carry out fermentation, and carrying out filtering after the fermentation is completed, thereby obtaining the wine. The vitis davidii wine obtained by the method is unique in taste and fragrance and has a certain healthcare action on human bodies, the category of the vitis davidii wine is enriched, the pollution rate of infectious microbes during brewing is lowered obviously, and thus, the vitis davidii wine has a very good market development value.

The invention discloses a grape cultivation process, which comprises the following steps: S1, robust and disease-free grape seedlings with complete main and lateral roots and fine roots are selected,the grape seedlings are planted in a nutrient matrix, the spacing between the grape seedlings is 80 cm, and the thickness of the nutrient matrix is 25 to 29 cm; S2, planting is carried out: fertile sandy loam soil is selected as a planting ground, a support is built on the planting ground, climbing of the grape seedlings is facilitated, a planting hole corresponding to the support is deeply dug beside the support, the root of the grape seedling is treated, and the treated grape seedling is planted in the planting hole; and S3, watering and fertilization are carried out: watering and fertilization are needed in the growing period of grape vines. The cost is saved while the grape output is ensured, manpower is reduced, human resources are saved, the fruit setting rate is improved, the fruitcracking rate is reduced, losses caused by diseases, insect pests and birds are lightened, cleanliness of the fruit surface is ensured, and functions of increasing the yield, improving the quality andincreasing the benefits are realized.

Protoporphyrinogen oxidase enzyme-inhibiting herbicides are useful in a method for controlling unwanted ground shoots of vines and other trunk vegetation. Of particular interest is the use of carfentrazone ethyl and certain metabolites thereof for control of unwanted ground shoots of vines and other trunk vegetation.

The invention discloses a making method of Vitis davidii wine. The making method comprises steps as follows: (1) material selection: 180-200 parts of wild Vitis davidii with bright color and without pest and disease damage are selected, and defected fruits, immature fruits and stems are rejected for later use; (2) pretreatment: the Vitis davidii obtained in step (1) is cleaned with clear water, drained and crushed with clean hands, 36-40 parts of white sugar and 0.54-0.6 parts of yeast are added during crushing, and the mixture is evenly stirred for later use; (3) fermentation: the mixture obtained in step (2) is poured into a fermentation barrel, the barrel opening is sealed with a plastic film, the plastic film sealing the barrel opening is fastened with rubber bands, the fastened fermentation barrel is moved into a fermentation room for fermentation for 11-13 days, and residue stirring is performed from the second day to the fourth day until fermentation is finished; (4) wine filtration: completely fermented wine in step (3) is filtered through a filter, and the Vitis davidii wine is made. The made wine has the strong fruit fragrance and tastes mellow, full and pleasant.

The invention discloses a traditional Chinese medicine composition for treating phlegm-stagnation and heat-arthralgia type rheumatic fever. The traditional Chinese medicine composition is characterized by being prepared from the following medicinal materials by weight: 6-9 parts of herba of zostera marina, 12-15 parts of bulb of picrarrhiza kurroa royle, 10-15 parts of frond of carrageen, 15-20 parts of herba of Abacopteris triphylla, 10-15 parts of luffah sponge, 6-9 parts of Zhejiang fritillary bulbs, 10-15 parts of Indian nightshade roots, 35-40 parts of herba or root of cayratia japonica, 15-20 parts of stem and root of schisandra lancifolia, 20-25 parts of water chestnut roots, 10-15 parts of herba of pilea martinii, 20-25 parts of stem or root of euphorbia humifusa, 12-15 parts of herba of elatostema lineolatum, 6-9 parts of herba of phlegmariurus fordii, 40-45 parts of vitis davidii roots and 3-6 parts of licorice roots. The traditional Chinese medicine composition, disclosed by the invention, is used for treating the phlegm-stagnation and heat-arthralgia type rheumatic fever and is high in effective rate and reliable in treatment effect, and recurrence does not occur any more after cure.

The invention provides a high-yield planting method of Vitis davidii Foex. The method comprises the steps: selecting Vitis davidii Foex seedlings which have a pure variety, robust growth, developed roots and high stress resistance, and performing trimming so as to improve the survival rate of the seedlings and make the variety better; selecting a suitable planting field, performing pre-treatment on soil, performing tillage multiple times, carrying out application of organic fertilizer and adjustment of the pH value of soil, and adding pesticide and fungicide to the soil so as to achieve pest killing and sterilization and remove pests and toxic bacteria, wherein adopted raw materials of the organic fertilizer are common, and a good fertilizer effect, better seedling growth and a higher yield are achieved; performing final planting and cultivation on the seedlings, and performing treatment on pests and diseases so as to reduce the pests and diseases of the seedlings; and performing dripirrigation or micro sprinkling irrigation on the seedlings, and rinsing front and rear surfaces of grape leaves so as to avoid the influence of rainwater on the grape quality, reduce pesticide residues and make the grapes meet safe and pollution-free requirements more. The method has simple operation, simple and easily available required materials and low cost, and is worth promoting.

The invention discloses a method for preparing resveratrol and viniferin from waste grape vine branches and tendrils obtained through pruning in autumn. The method comprises the steps: with fresh grape branches and tendrils obtained through pruning as raw materials, storing in a shady, cool and dark place, detecting contents of resveratrol and viniferin on time, after a given period, performing ultrasonic-assisted extraction, and performing adsorption separation through macroreticular resin to respectively obtain resveratrol and viniferin products. The yield of the obtained resveratrol reaches 1.1 percent, the content of the resveratrol reaches above 22 percent, the yield of the viniferin reaches 1.0 percent, and the content of the viniferin reaches above 20 percent. The method is capable of remarkably increasing the contents of the resveratrol and the viniferin in grape vines, low in energy consumption, high in separation efficiency, free from using an organic solvent, capable of recycling waste materials so that the environment pollution is reduced, simple in process and suitable for industrialized product.

The invention relates to the technical field of plant cultivation, in particular to a breeding method and a culture medium of vitis davidii seedlings. The culture medium is mainly composed of extract of, by weight, 8-12 parts of dried young and tender grape branches and leaves, 15-25 parts of green tea and 0.05-0.15 part of pseudo-ginseng. The extract is prepared by subjecting the grape branches and leaves, the green tea and the pseudo-ginseng to mixing, smashing, sieving and hot water extracting. The breeding method includes: inoculating vitis davidii explants into the vitis davidii seedling culture medium; culturing until seedling height is 5-10cm; hardening seedlings to obtain the vitis davidii seedlings. The breeding method is simple and easy to implement and quite high in qualified seedling percentage.

The invention discloses a preparation method of Vitis davidii Foex wine. The preparation method comprises the following seven steps of preparing materials, cleaning, crushing, fermenting, extracting supernate, aging and packaging. The preparation method effectively overcomes defects of short preservation time, weak original fruit fragrance, turbid and brown fruit wine, loss of ascorbic acid and the like. The finished product of Vitis davidii Foex wine has rose fragrance. The Vitis davidii Foex flesh contains higher sugar, thereby being used as a raw material of dry red wine and high-quality ruby red wine with high healthcare value. The Vitis davidii Foex wine is new in taste, contains no industrial raw materials or chemical raw materials, and is prepared through a physical processing method, thereby being healthy food.

The invention discloses a method for separating and preparing a caftaric acid standard product in a vitis davidii juice by using high-speed countercurrent chromatography. The method comprises the following steps: enriching the vitis davidii juice by using macroporous adsorption resin, eluting the material with ethanol, performing freeze-drying to obtain a crude sample, and performing high-speed countercurrent separation on the crude sample through solvent system water. The invention establishes the method for separating a polyphenol monomer in the vitis davidii juice by the high-speed countercurrent chromatography, and the method identifies the seperated monomer as caftaric acid by the methods such as HPLC-MS and nuclear magnetic resonance. The caftaric acid has an important pharmacological function, and its standard product is expensive, and the caftaric acid prepared by the method of the present invention has a purity of 95.8% and a calculated yield of 61.99 mg/L. The method of the high-purity standard product prepared can satisfy the large-scale preparation of caftaric acid of vitis davidii.

The invention provides a method for increasing the anthocyanin content of vitis davidii cell cultures. The method comprises the steps that a vitis davidii cell line stably showing purple red color in the secondary culture process is selected from cells which are subjected to previous-generation secondary culture for 20 days, cells of which the surface layers slightly have part of the inner layers are picked to serve as an inoculating material, a culture medium in which KT is not added is inoculated with the picked cells, culturing is conducted for 20 days, and the cultured cells are taken as a starting material for high-content anthocyanin cell inoculating; cells of which the surface layers slightly have part of the inner layers are picked from the starting material to serve as an inoculating material, a culture medium induced by high-content anthocyanin is inoculated with the picked cells, culturing is conducted for 3 days in low light or a dark environment at 25+/-1 DEG C and then continues to be conducted for 32 days under the condition that illumination is conducted for 12 h every day, and the vitis davidii cell cultures with the high-content anthocyanin are obtained. According to the method, not only can a large quantity of vitis davidii cell cultures be quickly obtained, but also the obtained vitis davidii cell cultures have the high-content anthocyanin, and then a good foundation is laid for natural anthocyanin production achieved through large-scale vitis davidii cell culture.