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Breeding method and culture medium of vitis davidii seedlings

A technology of thorny grape and culture medium, which is applied in the field of thorny grape seedling propagation method and culture medium, can solve the problems of poor rooting rate of thorny grape branch cuttings, lagging seedling raising technology, low seedling rate, etc., and achieve high uniformity, Strong stress resistance and quick return of seedlings

Active Publication Date: 2017-02-22
湖北蕊露源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the breeding of thorny grapes adopts the traditional cutting method, and the seedling rate of the seedlings is relatively low, mostly around 10%; The extremely poor rate and lagging seedling raising technology restrict the development of this industry

Method used

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  • Breeding method and culture medium of vitis davidii seedlings

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] This embodiment provides a method for propagating thorn grape seedlings and the medium used in the method.

[0064] 1. Culture medium preparation: Mix the dried young grape branches and leaves: green tea: Panax notoginseng in a mass ratio of 8:25:0.05, crush them, pass through a 20-mesh sieve, use 30 times of water, and extract at 90°C for 2 hours , filter, add 40g / L konjac fine powder to the filtrate, mix well, put it into a light-transmitting polytetrafluoroethylene culture box with a length × width × height of 20 × 20 × 15, the height of the medium is 2cm, and sterilize at 121°C After 30 minutes, place in a three-dimensional culture rack to cool for later use.

[0065] 2. Preparation of exogenous body: take materials in June. Take plump axillary buds, the axillary buds carry the phloem of the raw branches, the phloem length x width x thickness is 8mm x 4mm x 0.7mm, disinfect with 70% alcohol aqueous solution for 15 seconds, wash with sterile water 3 times for later ...

Embodiment 2

[0070] This embodiment provides a method for propagating thorn grape seedlings and the medium used in the method.

[0071] 1. Culture medium preparation: Mix the dried young grape branches and leaves: green tea: Panax notoginseng in a mass ratio of 12:15:0.15, crush them, pass through a 40-mesh sieve, use 30 times the water, and extract at 90°C for 2 hours , filter, add 60g / L konjac fine powder to the filtrate, mix well, put it into a light-transmitting polytetrafluoroethylene culture box with a length × width × height of 20 × 20 × 15, the height of the medium is 2cm, and sterilize at 121°C After 30 minutes, place in a three-dimensional culture rack to cool down for later use.

[0072] 2. Preparation of exogenous bodies: samples were collected in July. Take plump axillary buds, the axillary buds carry the phloem of the raw branches, the phloem length x width x thickness is 12mm x 2mm x 0.3mm, disinfect with 70% alcohol aqueous solution for 20 seconds, wash with sterile water ...

Embodiment 3

[0077] This embodiment provides a method for propagating thorn grape seedlings and the medium used in the method.

[0078] 1. Culture medium preparation: Mix the dried young grape branches and leaves: green tea: Panax notoginseng in a mass ratio of 10:20:0.1, crush them, pass through a 30-mesh sieve, use 30 times of water, and extract at 90°C for 2 hours , filter, add 50g / L konjac fine powder to the filtrate, mix well, put into a light-transmitting polytetrafluoroethylene culture box with a length × width × height of 20 × 20 × 15, the height of the medium is 2cm, and sterilize at 121°C After 30 minutes, place in a three-dimensional culture rack to cool down for later use.

[0079] 2. Preparation of exogenous cells: samples were collected in July. Take plump axillary buds, the axillary buds carry the phloem of the raw branches, the length of the bast × width × thickness is 10 mm × 3 mm × 0.5 mm, sterilize with 70% alcohol aqueous solution for 15 seconds, and wash with sterile ...

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Abstract

The invention relates to the technical field of plant cultivation, in particular to a breeding method and a culture medium of vitis davidii seedlings. The culture medium is mainly composed of extract of, by weight, 8-12 parts of dried young and tender grape branches and leaves, 15-25 parts of green tea and 0.05-0.15 part of pseudo-ginseng. The extract is prepared by subjecting the grape branches and leaves, the green tea and the pseudo-ginseng to mixing, smashing, sieving and hot water extracting. The breeding method includes: inoculating vitis davidii explants into the vitis davidii seedling culture medium; culturing until seedling height is 5-10cm; hardening seedlings to obtain the vitis davidii seedlings. The breeding method is simple and easy to implement and quite high in qualified seedling percentage.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for propagating thorny grape seedlings and a culture medium. Background technique [0002] Thorn grape (Vitis davidii Foex) is a wild germplasm resource of the East Asian population of the genus Vitis in the family Vitaceae. It is native to Hunan, Yunnan, Guangdong, Jiangxi, Zhejiang and other provinces in my country. It is a perennial deciduous vine. [0003] The fruit of thorn grapes is not only delicious, but also has high nutritional value. Ripe berries contain 15% to 25% glucose, vitamins, proteins, minerals potassium, phosphorus, iron, etc. In addition, it also contains a variety of substances with physiological functions. It has been scientifically proven that thorny grapes can prevent thrombosis better than aspirin, and can lower the serum cholesterol level of the human body, reduce the cohesion of platelets, and have a certain effect on the prevention...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 向极钎黎玥彤黎桓菲
Owner 湖北蕊露源生物科技有限公司
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