Method for breeding tomatoes having reduced water content and product of the method
a technology of tomato and water content, which is applied in the field of method for breeding tomatoes having reduced water content and product of the method, can solve the problems of general degradation of tomatoes, energy consumption, and use of drying ovens, and achieve the effect of reducing water content and water conten
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example 1
[0026] Plants of the L. esculentum breeding line 1630 (a Volcani Institute male sterile breeding line, used to simplify the production of the interspecific hybrid) were pollinated with pollen of the wild species L. hirsutum (LA1777). Hybrid F1 plants were grown and allowed to self-pollinate, generating F2 seed. F2 seed were sown and about 350 plants were grown in a screenhouse and allowed to self-pollinate. Ripe fruit from each individual plant that produced fruit were individually analyzed for soluble solids (refractometrically) to insure the lines also included the characteristic of high soluble solids. Only 25 of the interspecific F2 plants freely produced fruit. Three F2 plants were selected based on their high sugar content (Brix in excess of 10) when ripe. For example, fruit of F2-82 had 71 mg soluble sugar, composed of sucrose, glucose and fructose, per gram fresh weight of fruit, as determined by the method described herein below. F3 seeds were sown and ten plants of each of...
example 2
[0028] Pollen from one plant (F2-82) which was characterized by high soluble sugar level in the mature fruit (71 mg soluble sugar, composed of sucrose, glucose and fructose, per gram fresh weight of fruit) was used to pollinate two standard, industry type tomatoes (breeding lines A701 and 699) for the production of two backcross-F1 (BC-F1) populations. One-hundred BC-F2 plants from each of the two hybrids were grown and the presence of signs of fruit dehydration, evidenced by wrinkling of the fruit skin, were seen in fruit of plants from these F2 populations. This shows that even at early stages of a selection program, the trait can be selected for without large populations of plants.
experiment 3
[0029] Fruit of progeny of advanced lines derived from the lines described in experiment 1, that showed the characteristic to dehydrate on the vine, as evidenced by the wrinkling of the fruit were harvested and the juice pressed and Brix of the expressed juice was measured by a digital refractometer (Atago model X-1). The following table shows characteristic Brix values of some of the partially dehydrated tomato fruit (cherry size, approx. 15 g). Fruit were harvested when partially wrinkled but not fully dehydrated. The results of this experiment indicate that the trait of fruit dehydration and increase in Brix value is a selectable inherited trait. The parental selection (self of 1465-3) was partially dehydrated as was the F1 hybrid between 1465-3 and the cherry cultivar F139. This indicates that the trait is at least partially dominant in its inheritance pattern. Similarly, 3 representative plant selections from the F2 population (1730) derived from the self of the F1 (1465-3×F139...
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