Acquiring and subculture maintaining method for high yield oligomeric proanthocyanidins vitis davidii callus

A technology of callus and proanthocyanidins, applied in the direction of plant cells, etc., to achieve the effect of vigorous growth ability and high induction efficiency

Active Publication Date: 2014-10-15
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, the cell culture of grape thorns is still in the stage of callus induction and culture, and there are few reports of long-term subculture maintenance; the cell cultur

Method used

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  • Acquiring and subculture maintaining method for high yield oligomeric proanthocyanidins vitis davidii callus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0015] 1. Preparation of high-yield proanthocyanidin thorny grape callus

[0016] 1. Selection and pretreatment of experimental materials

[0017] Take the young thorny grapes about 20 days after flowering, cut the whole bunch of thorny grapes, put them in a ziplock bag, bring them back to the laboratory and store them in a refrigerator at 4°C for later use. Take out the young thorny grape fruit from the refrigerator, remove the ears and stalks, soak in tap water with detergent for 30 minutes, rinse with tap water, and dry naturally for later use. Put the young fruit into the ultra-clean workbench, soak it in 75% alcohol (v / v) for 30 seconds, then sterilize it with 0.1% mercury liter (w / v) solution for 15 minutes, and finally rinse it with sterile water for 5 times; The sterilized young thorn grapes are carefully cut open, and the intact young embryos are taken out.

[0018] 2. Initiate culture: take the immature embryos and inoculate them into the induction medium, and plac...

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Abstract

The invention provides an acquiring and subculture maintaining method for high yield oligomeric proanthocyanidins vitis davidii callus. The method comprises the steps of acquisition of sterile embryo, starting culture, enrichment culture, screening of vitis davidii callus high yield oligomeric proanthocyanidins cell lines, subculture maintaining of the screened high yield oligomeric proanthocyanidins callus in long term and the like. According to the invention, not only can a great amount of vitis davidii callus be obtained quickly, but also the acquired vitis davidii callus has the advantages of high inductive efficiency, exuberant growth and high yield of oligomeric proanthocyanidins; meanwhile, the vitis davidii callus can be conducted by subculture maintaining in long term, that is, exuberant growth capability is provided, so as to lay favorable foundation for study of secondary metabolites of vitis davidii biotechnology and cell culture and production.

Description

technical field [0001] The invention specifically relates to a method for obtaining and subculture maintaining high-yielding proanthocyanidin thorny grape callus. Background technique [0002] Thorn grape (Vitis davidii ) is a wild species of the East Asian population of the genus Vitis vinifera. It is a powerful deciduous vine, widely distributed in southern my country, and distributed northward to southern Shaanxi. The fruit of the thorn grape is purple-black, extremely nutritious, and contains flavonoids (mainly anthocyanins and proanthocyanidins), resveratrol, oleanolic acid, squid, superoxide dismutase (SOD) and Natural active ingredients such as vitamin C. The thorn grape has thick skin and many seeds, so it has a good application prospect in processing juice and wine, and extracting natural active substances from the skin and seeds. Studies have shown that the anthocyanins and proanthocyanidins (oligomeric proanthocyanidins, OPCs) rich in thorn grape fruit have a ...

Claims

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Application Information

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IPC IPC(8): C12N5/04
Inventor 赖呈纯范丽华黄贤贵谢鸿根
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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