Method for preparing two main anthocyanin standard substances of vitis davidii

Abstract

The invention discloses a method for preparing two main anthocyanin standard substances in cloudy juice of vitis davidii by utilizing high-speed counter-current chromatography. The method comprises the following steps: enriching the cloudy juice of vitis davidii by adopting a macroporous resin, eluting with ethanol, performing freeze drying to obtain a crude sample, performing high-speed counter-current separation on the crude sample with water in a solvent system, and separating to prepare anthocyanin standard substances, wherein the anthocyanin standard substances are respectively malvidin-[3-O-glucose-]-[5-O-glucose] with purity of 95.8% and malvidin-[3-O-beta-glucosyl-6-coumaroyl]-[5-O-beta-D-glucose] with purity of 92.2%. The yield of the two anthocyanin standards prepared from the cloudy juice of vitis davidii is respectively 122.77mg / L and 78.09 mg / L. A method for separating anthocyanin and polyphenol in the cloudy juice of vitis davidii by high-speed counter-current chromatography is established, and the method for preparing high-purity standard substances can meet large-scale preparation of natural anthocyanin of the vitis davidii.

Description

technical field [0001] The invention relates to a preparation method of standard anthocyanins, in particular to a method for preparing two main standard anthocyanins from thorn grape juice. Background technique [0002] The thorny grape (Vitis davidii Foex) belongs to the East Asian species of Vitis genus, and has a wide range of planting areas in Shaanxi, Gansu, Central China, South China and Southwest China. Among them, Hunan is one of the provinces where thorny grapes are concentrated and has a cultivation history of more than 200 years. [J]. Chinese and foreign grapes and wine, 2002 (02): 22-24]. A number of studies have shown that thorn grapes have high yield, strong resistance, good fruit flavor, more juice, high sugar-acid ratio, less tannin, and flavonoids mainly contain proanthocyanidins and resveratrol. Biologically active substances have great development space and greater application prospects. [0003] Anthocyanins are a class of compounds in which hydroxyl a...

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Problems solved by technology

Hu Xiaodan et al. used high-speed countercurrent chromatography to separate perilla leaf anthocyanins and malonyl perilla ning monomers from the crude extract of perilla leaf anthocyanins [Hu Xiaodan, Sun Aidong, Zhang Dequan. Study on the separation of perilla leaf anthocyanins by high-speed countercurrent chromatography ( English) [J]. Chinese Materia Medica, 2010,33(10): 1586-1588.] At present, the extraction of the main anthocyanins of thorny grapes has been reported, but it is suitable for the efficient preparation of standard products for liquid chromatography detection method not published yet

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