The invention relates to a high-expression recombination
protein carrier pRELPN. The high-expression recombination
protein carrier pRELPN is artificially synthesized ELP
fusion protein(RELPN) consisting of 9 polyarginine (R9)-
elastin polypeptide (ELP) n-10 poly
asparagine (N10), when multiple clone sites cloned in
pronucleus or eukaryotic expression vectors (p) are not directly expressed as exogenous genes, the effect of a second
promoter can be achieved. The pRELPN carrier can promote independent high expression of an exogenous
target gene behind start
cipher ATG cloned behind ELP
fusion protein. The
fusion protein is seldom expressed, and self expression of hosts of
escherichia coli or eukaryocyte and the like can be slightly restrained, so that recombination
protein accumulation and high expression of the exogenous
target gene can be promoted. The high
expression vector pRELPN containing dual promoters is suitable for independent high expression of exogenous genes of antibodies, antigens, enzymes, recombination protein, polypeptide, ELP fusion protein and the like, so as to contribute to solving of the problem of disindustrialization caused by that a common
expression vector islow or non in expression on an exogenous
gene an exogenous
gene or a
fusion gene consisting of the exogenous
gene and ELP.