Recombinant expression carrier and construction method and application thereof

A technology of recombinant vectors and expression vectors, applied in the field of genetic engineering, can solve the problems of low expression, inability to study functions, low expression of recombinant proteins, etc., and achieve the effect of promoting high expression
CN110343183AActive Publication Date: 2019-10-18点斗基因科技(南京)有限公司
8 Cites 6 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
点斗基因科技(南京)有限公司
Publication Date
2019-10-18

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a high-expression recombination protein carrier pRELPN. The high-expression recombination protein carrier pRELPN is artificially synthesized ELP fusion protein(RELPN) consisting of 9 polyarginine (R9)-elastin polypeptide (ELP) n-10 poly asparagine (N10), when multiple clone sites cloned in pronucleus or eukaryotic expression vectors (p) are not directly expressed as exogenous genes, the effect of a second promoter can be achieved. The pRELPN carrier can promote independent high expression of an exogenous target gene behind start cipher ATG cloned behind ELP fusion protein. The fusion protein is seldom expressed, and self expression of hosts of escherichia coli or eukaryocyte and the like can be slightly restrained, so that recombination protein accumulation and high expression of the exogenous target gene can be promoted. The high expression vector pRELPN containing dual promoters is suitable for independent high expression of exogenous genes of antibodies, antigens, enzymes, recombination protein, polypeptide, ELP fusion protein and the like, so as to contribute to solving of the problem of disindustrialization caused by that a common expression vector islow or non in expression on an exogenous gene an exogenous gene or a fusion gene consisting of the exogenous gene and ELP.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The application belongs to the technical field of genetic engineering, and relates to a high-expression recombinant expression vector pRELPN and its construction method and application. Background technique

[0002] Since the Danish geneticist Johansen (W. Johansen 1859~1927) formally proposed the concept of "gene" in his book "Principles of Precision Genetics" in 1909, after the 1950s, with the development of molecular genetics, especially After Watson and Crick proposed the DNA double helix structure, people used various methods to express various biological or human genes in bacteria (Escherichia coli), yeast, plants, insects and cell lines in order to study The function of the gene and the industrial application of the recombinant protein to promote the gene industry. At present, 75% of human-related proteins can be expressed in Escherichia coli, and many proteins have played a huge role in drug therapy. For example, human insulin for diabetes tre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More