Composition of collagen peptide and elastin peptide, and preparation method and application of composition

A technology of elastin peptide and collagen peptide, which is applied in the direction of chemical instruments and methods, biochemical equipment and methods, applications, etc., can solve the problems of high cost and increase consumer cost, and achieve the reduction of hydroxyproline content, inhibition of The effect of skin inflammatory factors, increased content

Inactive Publication Date: 2019-04-12
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the process preparation of elastin peptide and the factors that are favored at present, its cost is relatively high, and the premis

Method used

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  • Composition of collagen peptide and elastin peptide, and preparation method and application of composition
  • Composition of collagen peptide and elastin peptide, and preparation method and application of composition

Examples

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Embodiment 1

[0037] Embodiment 1: preparation composition of the present invention

[0038]Cut the cod skin into pieces, dry it in an oven at 80°C for 12 hours, then pulverize it into powder with a pulverizer, and store it in a cool and dry place for later use. Weigh cod skin powder, add 20-30 times the amount of water, heat the solution to 60°C±5°C, add 1% pepsin to the solution, enzymatically hydrolyze for 4-8 hours, heat up to 80±5°C, and keep warm for 0.5-1 hours, inactivate the enzyme, then pass the enzymolysis solution through a 1 μm filter membrane, maintain the temperature at 55±5°C, and take the filtrate. Collagen peptides were obtained after the filtrate was spray-dried. The molecular weight of collagen determined by high performance size exclusion chromatography is 2000Da~6000Da;

[0039] Take bovine ligament and wash it, add 0.5% NaOH solution, soak at 40-50°C for 1-2 hours, remove surface fat and impurities, wash to the center, drain and mince to obtain crude elastin extract...

Embodiment 2

[0041] Embodiment 2: animal experiments

[0042] 1. Experimental materials

[0043] D-galactose: purchased from Sigma-Aldrich; Mouse Hyaluronic acid (HA) ELISA kit, Mouse hydroxyproline (Hyp) ELISA kit, Mouse Collagen Type I (ColI) ELISA kit, MMP3 ELISA kit, Mouse Elastin ELISA kit, IL-1α ELISA kit The kit and IL-4 kit were purchased from Wuhan Huamei Bioengineering Co., Ltd.; GSH-PX was purchased from Nanjing Jiancheng Bioengineering Institute.

[0044] 2. Experimental equipment

[0045] Electronic balance: Mettler Toledo, model: PL303; skin moisture meter: Huntkey portable elastic moisture oily skin tester (HUNTKEY JAPAN Co., Ltd.), model: HKJ-SK03P; UV sterilization lamp: Philips black light tube TL-D ultraviolet sterilization Lamp (Philips Lighting Company), model: TL-D / BLB; centrifuge: Kylin-BellLabInstruments, model: LX-200; microplate reader: American BIO-TEK, model: ELX-800; homogenizer: SONICSMATERIALS INC, Model: VCX130PB; EP tube, syringe, etc.

[0046] 3. Exper...

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Abstract

The invention relates to the technical field of food and medicines, and discloses a composition of a collagen peptide and an elastin peptide, and a preparation method and application of the composition. The composition of the present invention is composed of a collagen peptide and an elastin peptide, wherein the collagen peptide is produced by enzymolysis of a collagen material by pepsin or trypsin, and the elastin peptide is prepared by enzymolysis of an elastin material via papain and/or a complex protease Protamex. According to the present invention, the elastin peptide and collagen peptidein specific molecular weight ranges are prepared by specific processes, and form the composition at a suitable ratio. The composition can significantly increase the content of elastin and collagen indamaged skin at the same time in a small amount of use, significantly increase the content of hyaluronic acid and hydroxyproline and decrease the content of MMP3, and also can inhibit skin inflammatory factors.

Description

technical field [0001] The invention relates to the technical fields of food and medicine, in particular to a composition of collagen peptide and elastin peptide, a preparation method and application thereof. Background technique [0002] Elastin is a protein that constitutes elastic fibers. It is widely distributed in various tissues and organs in the body, and mainly exists in elastic tissues such as cervical ligaments, blood vessels, lungs, and skin. Such tissues require not only elasticity but also tensile strength, and elastin plays an important role in the formation and maintenance of shape. Elastin is rich in hydrophobic amino acids such as glycine, alanine, and valine, and in the process of elastin biosynthesis, its precursor substance, tropoelastin, forms amino acids, desmosin, and isodesmosin through lysyl oxidase. Elastin and intervenes in the cross-linked structure composed of four lysine residues, so elastin helps to maintain the stability and elasticity of the...

Claims

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Application Information

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IPC IPC(8): A61K38/39A61P17/00A61P29/00A23L33/18
CPCA23V2002/00A61K38/39A23L33/18A61P17/00A61P29/00A61K2300/00A23V2200/318A61K8/64A61K8/645A61K9/0014A61K2800/10A61Q19/00C07K14/78C12Y304/21014C12Y304/22002A61L27/24A61L27/3604A61L27/3645A61L27/3687C12N9/6427C12N9/6481
Inventor 朱华伟汪晓凌丁刘刚郭晓蕾葛亚中
Owner INFINITUS (CHINA) CO LTD
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